| Definition | Escherichia coli HS, complete genome. |
|---|---|
| Accession | NC_009800 |
| Length | 4,643,538 |
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The map label for this gene is menD [H]
Identifier: 157161751
GI number: 157161751
Start: 2418376
End: 2420046
Strand: Reverse
Name: menD [H]
Synonym: EcHS_A2410
Alternate gene names: 157161751
Gene position: 2420046-2418376 (Counterclockwise)
Preceding gene: 157161752
Following gene: 157161750
Centisome position: 52.12
GC content: 57.93
Gene sequence:
>1671_bases ATGTCAGTAAGCGCATTTAACCGACGCTGGGCGGCGGTCATTCTGGAAGCATTAACGCGTCACGGCGTCAGACACATCTG TATCGCCCCAGGCTCGCGTTCTACACCGTTAACGTTAGCGGCGGCGGAGAATTCCGCATTCATTCACCACACCCATTTCG ATGAGCGTGGGTTGGGGCATCTGGCGCTGGGGCTGGCGAAAGTCAGCAAGCAGCCGGTGGCGGTGATTGTGACCTCCGGC ACGGCGGTGGCAAATCTCTATCCGGCACTGATTGAAGCCGGGTTAACCGGAGAAAAACTGATTCTCTTAACCGCCGATCG CCCGCCGGAGCTAATTGACTGCGGCGCGAATCAGGCAATTCGCCAGCCGGGAATGTTCGCCTCTCACCCCACGCACAGTA TTTCATTGCCGCGCCCGACCCAGGATATCCCCGCACGTTGGCTGGTTTCTACCATCGACCACGCTCTCGGTACGCTTCAT GCGGGGGGAGTCCATATCAACTGCCCGTTTGCTGAACCGCTGTATGGCGAAATGGACGATACCGGGCTTAGCTGGCAACA GCGTCTGGGTGACTGGTGGCAGGACGACAAACCGTGGCTGCGTGAAGCGCCTCGTCTGGAAAGTGAAAAACAGCGCGACT GGTTCTTCTGGCGACAAAAGCGCGGCGTGGTGGTTGCCGGGCGCATGAGTGCGGAAGAGGGCAAAAAAGTTGCCCTGTGG GCGCAAACTCTTGGCTGGCCGCTGATTGGCGATGTGCTGTCACAAACCGGGCAGCCGCTGCCGTGTGCCGATCTTTGGTT AGGCAATGCCAAAGCGACCAGCGAGCTGCAGCAGGCGCAAATTGTGGTGCAACTGGGAAGCAGCCTGACGGGGAAACGGC TCCTGCAATGGCAGGCAAGCTGTGAACCAGAAGAGTACTGGATTGTTGATGACATTGAAGGGCGACTTGATCCGGCACAC CATCGCGGACGTCGCTTAATTGCCAATATTGCCGACTGGCTGGAGCTGCATCCGGCAGAAAAACGCCAGCCCTGGTGCGT TGAAATCCCGCGCCTAGCGGAACAGGCAATGCAGGCGGTTATTGCCCGCCGTGATGCGTTTGGCGAAGCGCAACTGGCGC ATCGCATCTGCGACTATCTGCCTGAACAGGGGCAATTGTTTGTTGGTAACAGCCTGGTGGTACGTCTGATTGATGCGCTT TCGCAACTTCCGGCAGGTTACCCGGTGTACAGCAACCGAGGGGCCAGCGGTATCGACGGACTGCTTTCGACCGCCGCAGG CGTTCAGCGGGCAAGCGGCAAACCGACGCTGGCGATTGTGGGCGATCTCTCCGCACTTTACGATCTCAACGCGCTGGCGT TATTGCGTCAGGTTTCTGCGCCGCTGGTATTAATTGTGGTGAACAACAACGGCGGGCAAATTTTCTCGCTGTTGCCAACG CCGCAAAGCGAGCGTGAGCGTTTCTATCTGATGCCGCAAAACGTCCATTTTGAGCACGCCGCCGCGATGTTCGAGCTGAA ATATCATCGTCCGCAAAACTGGCAGGAACTTGAAACGGCATTTGCCGACGCCTGGCGCACGCCAACCACCACGGTGATTG AAATGGTGGTTAACGACACCGATGGTGCGCAAACGCTCCAGCAACTTCTGGCGCAGGTAAGCCATTTATGA
Upstream 100 bases:
>100_bases CAAATGGAATAGTAATGAGTCGCATCATTACCGATTCATATCAATAATCTATTTTTGTAGCTCCTTATACTTAGCCGCAA TATTGATACCGGACAAACTC
Downstream 100 bases:
>100_bases TCCTGCACGCGCAGGCAAAACACGGAAAACCAGGTTTACCCTGGCTGGTGTTTTTGCACGGTTTTTCCGGCGATTGCCAC GAATGGCAAGAAGTGGGCGA
Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase
Products: NA
Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD [H]
Number of amino acids: Translated: 556; Mature: 555
Protein sequence:
>556_residues MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAENSAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSG TAVANLYPALIEAGLTGEKLILLTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWRQKRGVVVAGRMSAEEGKKVALW AQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSELQQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAH HRGRRLIANIADWLELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRICDYLPEQGQLFVGNSLVVRLIDAL SQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLNALALLRQVSAPLVLIVVNNNGGQIFSLLPT PQSERERFYLMPQNVHFEHAAAMFELKYHRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL
Sequences:
>Translated_556_residues MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAENSAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSG TAVANLYPALIEAGLTGEKLILLTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWRQKRGVVVAGRMSAEEGKKVALW AQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSELQQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAH HRGRRLIANIADWLELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRICDYLPEQGQLFVGNSLVVRLIDAL SQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLNALALLRQVSAPLVLIVVNNNGGQIFSLLPT PQSERERFYLMPQNVHFEHAAAMFELKYHRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL >Mature_555_residues SVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAENSAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGT AVANLYPALIEAGLTGEKLILLTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLHA GGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWRQKRGVVVAGRMSAEEGKKVALWA QTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSELQQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHH RGRRLIANIADWLELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRICDYLPEQGQLFVGNSLVVRLIDALS QLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLNALALLRQVSAPLVLIVVNNNGGQIFSLLPTP QSERERFYLMPQNVHFEHAAAMFELKYHRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL
Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca
COG id: COG1165
COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the TPP enzyme family. MenD subfamily [H]
Homologues:
Organism=Escherichia coli, GI1788599, Length=556, Percent_Identity=100, Blast_Score=1135, Evalue=0.0,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR004433 - InterPro: IPR012001 - InterPro: IPR011766 [H]
Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N [H]
EC number: =2.2.1.9 [H]
Molecular weight: Translated: 61368; Mature: 61237
Theoretical pI: Translated: 6.55; Mature: 6.55
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.3 %Cys (Translated Protein) 1.4 %Met (Translated Protein) 2.7 %Cys+Met (Translated Protein) 1.3 %Cys (Mature Protein) 1.3 %Met (Mature Protein) 2.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAENSAFIHHTHFDERGLGH CCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCCEEEEECCCCCCCCHH LALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLILLTADRPPELIDCGANQAI HHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHEECCCCHHH RQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLHAGGVHINCPFAEPLYGEMDD HCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCEECCCEEEECCCCCCCCCCCCC TGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWRQKRGVVVAGRMSAEEGKKVALW CCCHHHHHCCCCCCCCCCHHHCCCCCCCCHHHHHHHEECCCCEEEEECCCCCCCCEEEEE AQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSELQQAQIVVQLGSSLTGKRLLQWQAS HHHCCCHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC CEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWLELHPAEKRQPWCVEIPRLAEQAMQAV CCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHH IARRDAFGEAQLAHRICDYLPEQGQLFVGNSLVVRLIDALSQLPAGYPVYSNRGASGIDG HHHHHCCCHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH LLSTAAGVQRASGKPTLAIVGDLSALYDLNALALLRQVSAPLVLIVVNNNGGQIFSLLPT HHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCC PQSERERFYLMPQNVHFEHAAAMFELKYHRPQNWQELETAFADAWRTPTTTVIEMVVNDT CCCCCCEEEECCCCCCHHHHHHHHEEECCCCCCHHHHHHHHHHHCCCCHHHEEEEECCCC DGAQTLQQLLAQVSHL CHHHHHHHHHHHHHCC >Mature Secondary Structure SVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAENSAFIHHTHFDERGLGH CCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCCEEEEECCCCCCCCHH LALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLILLTADRPPELIDCGANQAI HHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHEECCCCHHH RQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLHAGGVHINCPFAEPLYGEMDD HCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCEECCCEEEECCCCCCCCCCCCC TGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWRQKRGVVVAGRMSAEEGKKVALW CCCHHHHHCCCCCCCCCCHHHCCCCCCCCHHHHHHHEECCCCEEEEECCCCCCCCEEEEE AQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSELQQAQIVVQLGSSLTGKRLLQWQAS HHHCCCHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC CEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWLELHPAEKRQPWCVEIPRLAEQAMQAV CCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHH IARRDAFGEAQLAHRICDYLPEQGQLFVGNSLVVRLIDALSQLPAGYPVYSNRGASGIDG HHHHHCCCHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH LLSTAAGVQRASGKPTLAIVGDLSALYDLNALALLRQVSAPLVLIVVNNNGGQIFSLLPT HHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCC PQSERERFYLMPQNVHFEHAAAMFELKYHRPQNWQELETAFADAWRTPTTTVIEMVVNDT CCCCCCEEEECCCCCCHHHHHHHHEEECCCCCCHHHHHHHHHHHCCCCHHHEEEEECCCC DGAQTLQQLLAQVSHL CHHHHHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA