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The map label for this gene is menF

Identifier: 157161752

GI number: 157161752

Start: 2420135

End: 2421430

Strand: Reverse

Name: menF

Synonym: EcHS_A2411

Alternate gene names: 157161752

Gene position: 2421430-2420135 (Counterclockwise)

Preceding gene: 157161753

Following gene: 157161751

Centisome position: 52.15

GC content: 53.47

Gene sequence:

>1296_bases
GTGCAATCACTTACTACGGCGCTGGAAAATCTACTGCGCCATTTGTCGCAAGAGATTCCGGCGACACCCGGCATTCGGGT
TATCGATATTCCTTTCCCTCTCAAAGACGCTTTTGATGCCTTGAGCTGGCTGGCCAGTCAGCAAACATACCCGCAATTCT
ACTGGCAACAACGTAATGGTGATGAAGAAGCTGTCGTCCTGGGCGCGATTACCCGTTTTACGTCGTTGGACCAGGCACAA
CGTTTTCTTCGCCAGCACCCGGAACACGCCGACTTACGCATTTGGGGGCTGAATGCTTTTGACCCGTCGCAGGGCAATTT
ACTTTTACCCCGCCTGGAATGGCGACGCTGTGGCGGTAAAGCCACGCTGCGGCTGACGCTATTCAGCGAAAGCTCCCTTC
AGCACGATGCGATTCAGGCAAAAGAATTTATCGCCACACTGGTGAGTATCAAGCCCTTGCCTGGGTTACATTTAACCACC
ACGCGAGAACAACACTGGCCGGACAAAACGGGCTGGACGCAATTAATCGAACTGGCAACGAAAACCATCGCCGAAGGTGA
GCTCGACAAAGTGGTGCTCGCTCGGGCAACTGACCTGCATTTCGCAAGTCCGGTCAACGCGGCGGCGATGATGGCTGCCA
GTCGTCGACTGAATCTGAATTGCTACCATTTTTACATGGCCTTTGATGGCGAAAATGCTTTTCTTGGCTCTTCACCGGAA
CGGTTATGGCGGCGGCGTGACAAAGCGCTGCGTACTGAAGCGCTGGCGGGAACAGTAGCAAATAATCCTGATGATAAGCA
GGCGCAGCAGTTAGGAGAGTGGCTGATGGCGGATGATAAAAACCAGCGCGAGAACATGCTGGTGGTGGAAGATATCTGCC
AACGATTACAGGCCGATACCCAGACGCTGGATGTTTTACCGCCGCAGGTACTGCGTCTGCGTAAAGTGCAGCATCTTCGC
CGCTGTATCTGGACTTCACTCAACAAAGCGGATGATGTGATCTGTTTACATCAGTTGCAGCCAACGGCAGCAGTTGCTGG
CTTACCGCGCGATCTGGCGCGACAGTTTATCGCCCGTCACGAACCGTTCACCCGAGAATGGTACGCCGGTTCTGCGGGCT
ATCTCTCATTACAACAAAGCGAATTCTGCGTTTCCCTGCGCTCAGCAAAAATTAGCGGCAATGTCGTGCGATTATATGCT
GGCGCGGGCATTGTCCGTGGTTCCGACCCCGAGCAAGAGTGGCAGGAAATCGACAACAAAGCGGCAGGGCTGCGTACTTT
ATTACAAATGGAATAG

Upstream 100 bases:

>100_bases
ACTGTTGCTGGCACGCCGTTAAAACCTCCCTACACCGGGGTACTGCATTTTTTTTGTAGTACCCCGTATAATGTGAGGCT
TTTAACAGGGAGAGGTCCGC

Downstream 100 bases:

>100_bases
TAATGAGTCGCATCATTACCGATTCATATCAATAATCTATTTTTGTAGCTCCTTATACTTAGCCGCAATATTGATACCGG
ACAAACTCATGTCAGTAAGC

Product: menaquinone-specific isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWLASQQTYPQFYWQQRNGDEEAVVLGAITRFTSLDQAQ
RFLRQHPEHADLRIWGLNAFDPSQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLNLNCYHFYMAFDGENAFLGSSPE
RLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLMADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLR
RCIWTSLNKADDVICLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKISGNVVRLYA
GAGIVRGSDPEQEWQEIDNKAAGLRTLLQME

Sequences:

>Translated_431_residues
MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWLASQQTYPQFYWQQRNGDEEAVVLGAITRFTSLDQAQ
RFLRQHPEHADLRIWGLNAFDPSQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLNLNCYHFYMAFDGENAFLGSSPE
RLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLMADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLR
RCIWTSLNKADDVICLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKISGNVVRLYA
GAGIVRGSDPEQEWQEIDNKAAGLRTLLQME
>Mature_431_residues
MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWLASQQTYPQFYWQQRNGDEEAVVLGAITRFTSLDQAQ
RFLRQHPEHADLRIWGLNAFDPSQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLNLNCYHFYMAFDGENAFLGSSPE
RLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLMADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLR
RCIWTSLNKADDVICLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKISGNVVRLYA
GAGIVRGSDPEQEWQEIDNKAAGLRTLLQME

Specific function: Menaquinone biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family

Homologues:

Organism=Escherichia coli, GI87082077, Length=431, Percent_Identity=100, Blast_Score=887, Evalue=0.0,
Organism=Escherichia coli, GI1786809, Length=276, Percent_Identity=31.5217391304348, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1787518, Length=299, Percent_Identity=27.7591973244147, Blast_Score=72, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6320935, Length=327, Percent_Identity=27.5229357798165, Blast_Score=77, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MENF_ECOLI (P38051)

Other databases:

- EMBL:   Z50849
- EMBL:   U54790
- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   U58768
- EMBL:   M21787
- PIR:   G64997
- RefSeq:   AP_002863.1
- RefSeq:   NP_416768.4
- PDB:   2EUA
- PDB:   3BZM
- PDB:   3BZN
- PDBsum:   2EUA
- PDBsum:   3BZM
- PDBsum:   3BZN
- ProteinModelPortal:   P38051
- SMR:   P38051
- DIP:   DIP-10187N
- MINT:   MINT-1275996
- STRING:   P38051
- PhosSite:   P38051
- EnsemblBacteria:   EBESCT00000004604
- EnsemblBacteria:   EBESCT00000018312
- GeneID:   946712
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2260
- KEGG:   eco:b2265
- EchoBASE:   EB2265
- EcoGene:   EG12362
- eggNOG:   COG1169
- GeneTree:   EBGT00050000009519
- HOGENOM:   HBG405459
- OMA:   WYAGTLG
- ProtClustDB:   PRK15012
- BioCyc:   EcoCyc:MENF-MONOMER
- BioCyc:   MetaCyc:MENF-MONOMER
- Genevestigator:   P38051
- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561
- Gene3D:   G3DSA:3.60.120.10
- PANTHER:   PTHR11236:SF3
- PANTHER:   PTHR11236
- TIGRFAMs:   TIGR00543

Pfam domain/function: PF00425 Chorismate_bind; SSF56322 TRPE_1_chor_bd

EC number: =5.4.4.2

Molecular weight: Translated: 48765; Mature: 48765

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWLASQQTYPQFYWQQRNG
CCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHCCCC
DEEAVVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDPSQGNLLLPRLEWRRCGGK
CCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEECCCHHHHHCCCC
ATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTTTREQHWPDKTGWTQLIELAT
EEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHCCCCCCHHHHHHHHHH
KTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLNLNCYHFYMAFDGENAFLGSSPE
HHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCHH
RLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLMADDKNQRENMLVVEDICQRLQADT
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHCCC
QTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVICLHQLQPTAAVAGLPRDLARQFIARH
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHCCCHHHHHHHHHHC
EPFTREWYAGSAGYLSLQQSEFCVSLRSAKISGNVVRLYAGAGIVRGSDPEQEWQEIDNK
CCCCHHHCCCCCCEEEECHHHHHHHHHHCCCCCCEEEEEECCCEECCCCCHHHHHHHHHH
AAGLRTLLQME
HHHHHHHHHCC
>Mature Secondary Structure
MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWLASQQTYPQFYWQQRNG
CCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHCCCC
DEEAVVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDPSQGNLLLPRLEWRRCGGK
CCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEECCCHHHHHCCCC
ATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTTTREQHWPDKTGWTQLIELAT
EEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHCCCCCCHHHHHHHHHH
KTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLNLNCYHFYMAFDGENAFLGSSPE
HHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCHH
RLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLMADDKNQRENMLVVEDICQRLQADT
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHCCC
QTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVICLHQLQPTAAVAGLPRDLARQFIARH
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHCCCHHHHHHHHHHC
EPFTREWYAGSAGYLSLQQSEFCVSLRSAKISGNVVRLYAGAGIVRGSDPEQEWQEIDNK
CCCCHHHCCCCCCEEEECHHHHHHHHHHCCCCCCEEEEEECCCEECCCCCHHHHHHHHHH
AAGLRTLLQME
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8549818; 8764478; 9205837; 9278503; 2666397; 7984428