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The map label for this gene is apbE [H]

Identifier: 157161696

GI number: 157161696

Start: 2351460

End: 2352515

Strand: Reverse

Name: apbE [H]

Synonym: EcHS_A2353

Alternate gene names: 157161696

Gene position: 2352515-2351460 (Counterclockwise)

Preceding gene: 157161697

Following gene: 157161695

Centisome position: 50.66

GC content: 53.03

Gene sequence:

>1056_bases
ATGGAAATAAGCTTTACCCGCGTGGCACTGCTGGCTGCCGCGCTCTTTTTTGTTGGTTGCGATCAAAAACCACAACCCGC
CAAAACCCACGCTACTGAAGTTACCGTTCTTGAAGGCAAAACCATGGGTACCTTCTGGCGTGCCAGCATCCCGGGCATTG
ATGCCAAACGCAGTGCCGAACTTAAAGAAAAGATTCAGACCCAGCTGGACGCCGACGATCAGCTGCTTTCGACCTATAAA
AAAGATTCCGCGCTGATGTGCTTTAACGATTCGCAAAGTTTATCGCCGTGGCCGGTAAGTGAAGCGATGGCCGATATCGT
CACCACCTCGCTGCGCATTGGCGCGAAGACCGATGGCGCGATGGATATAACCGTCGGGCCGCTGGTGAATCTGTGGGGCT
TTGGCCCGGAACAACAGCCGGTTCAAATTCCGAGCCAGGAACAGATCGATGCGATGAAAGCCAAAACCGGCTTACAGCAC
CTGACGGTCATCAATCAGTCGCATCAGCAATATCTGCAAAAAGACCTGCCGGATTTATATGTCGATCTCTCCACCGTCGG
TGAAGGTTATGCGGCGGATCACCTGGCACGCTTGATGGAGCAGGAAGGGATTTCCCGCTATCTGGTGTCGGTGGGCGGCG
CGCTGAACAGCCGTGGTATGAACGGTGAAGGCCAGCCGTGGCGGGTAGCGATTCAAAAACCAACCGATAAAGAAAACGCG
GTTCAAGCTGTGGTGGATATCAACGGTCATGGGATTAGCACCTCTGGCAGCTACCGTAACTATTACGAACTGGACGGCAA
ACGTCTTTCCCATGTTATCGATCCGCAAACCGGGCGTCCCATCGAACACAATCTGGTATCCGTGACGGTGATTGCTCCGA
CGGCGCTGGAAGCCGATGCCTGGGATACTGGCTTAATGGTACTCGGGCCGGAGAAAGCCAAAGAGGTTGTTCGCCGGGAA
GGGCTGGCGGTTTATATGATCACCAAAGAAGGCGATAGCTTTAAAACCTGGATGTCACCACAGTTTAAAAGCTTCCTTGT
CAGCGAAAAAAATTAA

Upstream 100 bases:

>100_bases
ATCGAACAAAGGGCCTGCGGGCCCTTTTTTCATTGTTTTCAGCGTACAAACACAGTTTTTTGGTGTACTCTTGCGACCGT
TCGCATGAGGATAATCACGT

Downstream 100 bases:

>100_bases
AGCGCAAGATTGTTGGTTTTTGCGTGATGGTGACCGGGCAGACTAAAGGCTATCCTTAACCAGGGAGCTGATTATGAAAA
ACGCCACATGCTTAACTGAC

Product: thiamine biosynthesis lipoprotein ApbE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGTFWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYK
KDSALMCFNDSQSLSPWPVSEAMADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSRGMNGEGQPWRVAIQKPTDKENA
VQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQTGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRRE
GLAVYMITKEGDSFKTWMSPQFKSFLVSEKN

Sequences:

>Translated_351_residues
MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGTFWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYK
KDSALMCFNDSQSLSPWPVSEAMADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSRGMNGEGQPWRVAIQKPTDKENA
VQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQTGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRRE
GLAVYMITKEGDSFKTWMSPQFKSFLVSEKN
>Mature_351_residues
MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGTFWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYK
KDSALMCFNDSQSLSPWPVSEAMADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSRGMNGEGQPWRVAIQKPTDKENA
VQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQTGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRRE
GLAVYMITKEGDSFKTWMSPQFKSFLVSEKN

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=351, Percent_Identity=99.4301994301994, Blast_Score=720, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 38511; Mature: 38511

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGTFWRASIPGIDAKRSAE
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCEEECCCCCCCCHHHHH
LKEKIQTQLDADDQLLSTYKKDSALMCFNDSQSLSPWPVSEAMADIVTTSLRIGAKTDGA
HHHHHHHHCCCCHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHEECCCCCCE
MDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQHLTVINQSHQQYLQKDLPDLY
EEEEHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHE
VDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSRGMNGEGQPWRVAIQKPTDKENA
EEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHCC
VQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQTGRPIEHNLVSVTVIAPTALEADA
EEEEEEECCCCCCCCCCCCCEEECCCHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCC
WDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSFKTWMSPQFKSFLVSEKN
CCCCEEEECCHHHHHHHHHCCCEEEEEECCCCCCHHHCCHHHHHHHCCCCC
>Mature Secondary Structure
MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGTFWRASIPGIDAKRSAE
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCEEECCCCCCCCHHHHH
LKEKIQTQLDADDQLLSTYKKDSALMCFNDSQSLSPWPVSEAMADIVTTSLRIGAKTDGA
HHHHHHHHCCCCHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHEECCCCCCE
MDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQHLTVINQSHQQYLQKDLPDLY
EEEEHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHE
VDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSRGMNGEGQPWRVAIQKPTDKENA
EEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHCC
VQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQTGRPIEHNLVSVTVIAPTALEADA
EEEEEEECCCCCCCCCCCCCEEECCCHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCC
WDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSFKTWMSPQFKSFLVSEKN
CCCCEEEECCHHHHHHHHHCCCEEEEEECCCCCCHHHCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]