Click here to switch to the map view.

The map label for this gene is yeiA

Identifier: 157161627

GI number: 157161627

Start: 2277664

End: 2278899

Strand: Direct

Name: yeiA

Synonym: EcHS_A2281

Alternate gene names: 157161627

Gene position: 2277664-2278899 (Clockwise)

Preceding gene: 157161626

Following gene: 157161634

Centisome position: 49.05

GC content: 51.29

Gene sequence:

>1236_bases
ATGTTAACGAAAGATCTTTCGATTACCTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGG
CAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGCGTGGTGTTTAAAACGATCGGCTTTTTTATCG
CCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATT
GCCGAGCATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTC
GATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTA
ACTTCTCCTGTCCGCAAATGACGTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGT
CGGGCAGTTAAACGGGGTTCCACGCTGCCGATGCTGGCGAAGATGACACCGAATATCGGTGATATGTGCGAAGTGGCGCT
GGCGGCGAAGCGCGGCGGCGCAGATGGCATTGCGGCAATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGA
AAATCGGTATGCCGATCGTGAACGGTAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGC
TTTATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCGATCAGCGGTATCGGCGGCATTGAAACCTGGGAAGA
TGCGGCGGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTCACCACCGGCATTATGCAGTACGGCTATCGGATAGTGG
AAGATATGGCGAGCGGGTTGTCGCATTATCTCGCCGATCAAGGGTTTGATTCGTTGCAGGAGATGGTAGGTCTGGCGAAT
AACAATATTGTCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGTGTTGGCTG
TGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTA
ATACCGAGAAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCGGTGGGTTGTATTGAGCTCGGGGAAGTGAAGTTT
AAGAAAGGCGAGAAAGAACACCCGGTAACGTTGTAA

Upstream 100 bases:

>100_bases
CTGGCGATATTGTTGAGGGTGACAAAACCGTGGTCTATGCCGTGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCAC
TATTTAGAGGGAGCTTGCTC

Downstream 100 bases:

>100_bases
AACCAGAGACGCATCCGGCATTTGGCACCGATTGCCGGATGCAGCGCATCCGGCAATATCCACAATCACATCACTTCTTA
CGCGCGTATTTCAGTGAATC

Product: dihydropyrimidine dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGVVFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQI
AEHPLEENLAALRRLKEDYPDKVLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMPIVNGKSSISGYSGKAVKPIALR
FIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGAATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLAN
NNIVPAEDLDRSYIVYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGCIELGEVKF
KKGEKEHPVTL

Sequences:

>Translated_411_residues
MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGVVFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQI
AEHPLEENLAALRRLKEDYPDKVLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMPIVNGKSSISGYSGKAVKPIALR
FIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGAATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLAN
NNIVPAEDLDRSYIVYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGCIELGEVKF
KKGEKEHPVTL
>Mature_411_residues
MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGVVFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQI
AEHPLEENLAALRRLKEDYPDKVLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMPIVNGKSSISGYSGKAVKPIALR
FIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGAATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLAN
NNIVPAEDLDRSYIVYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGCIELGEVKF
KKGEKEHPVTL

Specific function: Unknown

COG id: COG0167

COG function: function code F; Dihydroorotate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Homo sapiens, GI119943098, Length=328, Percent_Identity=35.9756097560976, Blast_Score=196, Evalue=3e-50,
Organism=Escherichia coli, GI87082059, Length=411, Percent_Identity=100, Blast_Score=857, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71984108, Length=324, Percent_Identity=39.8148148148148, Blast_Score=214, Evalue=6e-56,
Organism=Saccharomyces cerevisiae, GI6322633, Length=241, Percent_Identity=27.8008298755187, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24640763, Length=315, Percent_Identity=38.7301587301587, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI18858217, Length=315, Percent_Identity=38.7301587301587, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI281361352, Length=337, Percent_Identity=24.3323442136499, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17137316, Length=337, Percent_Identity=24.3323442136499, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YEIA_ECOLI (P25889)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   M59444
- RefSeq:   AP_002744.1
- RefSeq:   NP_416652.4
- ProteinModelPortal:   P25889
- SMR:   P25889
- DIP:   DIP-11915N
- IntAct:   P25889
- STRING:   P25889
- EnsemblBacteria:   EBESCT00000001392
- EnsemblBacteria:   EBESCT00000016623
- GeneID:   949037
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2134
- KEGG:   eco:b2147
- EchoBASE:   EB1266
- EcoGene:   EG11289
- eggNOG:   COG0167
- GeneTree:   EBGT00050000010648
- HOGENOM:   HBG472415
- OMA:   MVTRWCK
- ProtClustDB:   PRK08318
- BioCyc:   EcoCyc:EG11289-MONOMER
- BioCyc:   MetaCyc:EG11289-MONOMER
- Genevestigator:   P25889
- GO:   GO:0005737
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013785
- InterPro:   IPR005720
- InterPro:   IPR012135
- InterPro:   IPR012285
- Gene3D:   G3DSA:3.20.20.70
- Gene3D:   G3DSA:1.10.1060.10
- PIRSF:   PIRSF000164
- TIGRFAMs:   TIGR01037

Pfam domain/function: PF01180 DHO_dh

EC number: NA

Molecular weight: Translated: 45069; Mature: 45069

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
8.3 %Cys+Met (Translated Protein)
4.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
8.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGVVFKTIGFFIANEVSPR
CCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHH
FDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDKVLIASIMGENEQQWEELA
HHHHHHHCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHCCCCEEHHHHHCCCHHHHHHHH
RLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYCRAVKRGSTLPMLAKMTPNIG
HHHHHCCCCEEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHCCCCHH
DMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMPIVNGKSSISGYSGKAVKPIALR
HHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHH
FIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGAATLQVTTGIMQYGYRIVEDMASGL
HHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYIVYPRINLDKCVGCGRCYISCYDGGHQ
HHHHHHCCHHHHHHHHHHCCCCCCCHHHCCCCEEEEECCCHHHHCCCCEEEEEEECCCCH
AMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGCIELGEVKFKKGEKEHPVTL
HHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGVVFKTIGFFIANEVSPR
CCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHH
FDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDKVLIASIMGENEQQWEELA
HHHHHHHCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHCCCCEEHHHHHCCCHHHHHHHH
RLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYCRAVKRGSTLPMLAKMTPNIG
HHHHHCCCCEEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHCCCCHH
DMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMPIVNGKSSISGYSGKAVKPIALR
HHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHH
FIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGAATLQVTTGIMQYGYRIVEDMASGL
HHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYIVYPRINLDKCVGCGRCYISCYDGGHQ
HHHHHHCCHHHHHHHHHHCCCCCCCHHHCCCCEEEEECCCHHHHCCCCEEEEEEECCCCH
AMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGCIELGEVKFKKGEKEHPVTL
HHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 1719366