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The map label for this gene is fghA1 [H]

Identifier: 157161634

GI number: 157161634

Start: 2286137

End: 2286973

Strand: Direct

Name: fghA1 [H]

Synonym: EcHS_A2288

Alternate gene names: 157161634

Gene position: 2286137-2286973 (Clockwise)

Preceding gene: 157161627

Following gene: 157161640

Centisome position: 49.23

GC content: 54.48

Gene sequence:

>837_bases
ATGGAAATGCTCGAAGAGCACCGCTGTTTTGAAGGCTGGCAGCAACGCTGGCGACACGACTCCAGTACCTTAAACTGCCC
GATGACGTTCAGTATCTTTCTCCCTCCACCTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCT
GCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTACTGGTGATGCCAGAC
ACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCAC
GCAACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTA
ATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGTGCGCTGATTATGGCGCTGAAAAATCCGGGT
AAATACACCAGCGTTTCGGCCTTTGCGCCAATTGTGAATCCGTGCAGCGTCCCGTGGGGAATCAAAGCGTTTAGCAGCTA
TTTAGGTGAGGACAAAAATGCATGGCTGGAATGGGACAGTTGCGCACTGATGTATGCCAGTAACGCGCAGGATGCGATCC
CGACGCTTATCGATCAGGGCGATAATGATCAGTTTCTTGCCGACCAGTTGCAACCTGCGGTACTGGCAGAAGCCGCGCGC
CAGAAAGCGTGGCCGATGACGCTGCGTATTCAGCCGGGATATGATCACAGTTACTACTTCATCGCCTCTTTTATAGAGGA
TCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGA

Upstream 100 bases:

>100_bases
TAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAAC
AACACAACAAGGAGCCACGC

Downstream 100 bases:

>100_bases
AAGTCCGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTA
TAGCTGTAGTCGTCACGACT

Product: S-formylglutathione hydrolase

Products: NA

Alternate protein names: FGH [H]

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPPVLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPD
TSPRGEKVANDDGYDLGQGAGFYLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLIDQGDNDQFLADQLQPAVLAEAAR
QKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYLLK

Sequences:

>Translated_278_residues
MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPPVLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPD
TSPRGEKVANDDGYDLGQGAGFYLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLIDQGDNDQFLADQLQPAVLAEAAR
QKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYLLK
>Mature_278_residues
MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPPVLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPD
TSPRGEKVANDDGYDLGQGAGFYLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLIDQGDNDQFLADQLQPAVLAEAAR
QKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYLLK

Specific function: Serine hydrolase involved in the detoxification of formaldehyde. Hydrolyzes S-formylglutathione to glutathione and formate [H]

COG id: COG0627

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the esterase D family [H]

Homologues:

Organism=Homo sapiens, GI33413400, Length=279, Percent_Identity=52.6881720430108, Blast_Score=304, Evalue=6e-83,
Organism=Escherichia coli, GI1788477, Length=278, Percent_Identity=100, Blast_Score=585, Evalue=1e-168,
Organism=Escherichia coli, GI1786551, Length=276, Percent_Identity=53.6231884057971, Blast_Score=320, Evalue=6e-89,
Organism=Caenorhabditis elegans, GI17510185, Length=280, Percent_Identity=45.7142857142857, Blast_Score=244, Evalue=5e-65,
Organism=Saccharomyces cerevisiae, GI6322393, Length=297, Percent_Identity=44.4444444444444, Blast_Score=238, Evalue=5e-64,
Organism=Drosophila melanogaster, GI45551932, Length=283, Percent_Identity=50.1766784452297, Blast_Score=268, Evalue=3e-72,
Organism=Drosophila melanogaster, GI24648347, Length=283, Percent_Identity=50.1766784452297, Blast_Score=268, Evalue=4e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000801
- InterPro:   IPR014186 [H]

Pfam domain/function: PF00756 Esterase [H]

EC number: =3.1.2.12 [H]

Molecular weight: Translated: 31259; Mature: 31259

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPPVLYWLSGLTCNDENFT
CCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEECCCEECCCCCC
TKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGFYLNATQPPWATHYRMYDY
CHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHH
LRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPGKYTSVSAFAPIVNPCSVPWG
HHHHHHHHHHHHCCCCCCEEECCCCCCCCCEEEEEECCCCCCCCHHHHHCCCCCCCCCCC
IKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLIDQGDNDQFLADQLQPAVLAEAAR
HHHHHHHHCCCCCCEEEECCEEEEEECCCCHHHHHHHCCCCCCCHHHHHCCHHHHHHHHH
QKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYLLK
HCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPPVLYWLSGLTCNDENFT
CCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEECCCEECCCCCC
TKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGFYLNATQPPWATHYRMYDY
CHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHH
LRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPGKYTSVSAFAPIVNPCSVPWG
HHHHHHHHHHHHCCCCCCEEECCCCCCCCCEEEEEECCCCCCCCHHHHHCCCCCCCCCCC
IKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLIDQGDNDQFLADQLQPAVLAEAAR
HHHHHHHHCCCCCCEEEECCEEEEEECCCCHHHHHHHCCCCCCCHHHHHCCHHHHHHHHH
QKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYLLK
HCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA