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The map label for this gene is ansA [H]

Identifier: 157161229

GI number: 157161229

Start: 1868942

End: 1869958

Strand: Direct

Name: ansA [H]

Synonym: EcHS_A1851

Alternate gene names: 157161229

Gene position: 1868942-1869958 (Clockwise)

Preceding gene: 157161228

Following gene: 157161230

Centisome position: 40.25

GC content: 50.44

Gene sequence:

>1017_bases
ATGCAAAAGAAATCAATTTACGTCGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATTCCGGT
GTCAGGTCATCTACAACGCCAACTGGCGCTGATGCCGGAATTCCATCGCCCGGAGATGCCAGATTTCACCATTCATGAAT
ATACGCCGTTGATGGATTCTTCTGATATGACTCCAGAAGACTGGCAGCATATTGCTGAAGATATTAAAGCGCACTATGAC
GACTATGATGGTTTTGTCATTCTGCACGGCACCGATACGATGGCATATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAA
TCTCGGTAAACCAGTCATTGTGACAGGGTCACAAATCCCGCTAGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGA
ATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGC
ACTACCAAAGCCCATGCCGATGGTTTTGATGCGTTTGCCTCTCCAAACCTTCCTCCGTTACTGGAAGCAGGTATCCATAT
TCGTCGTTTAAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTGGCG
TAGTGACGATTTATCCAGGGATTTCTGCTGACGTCGTGCGCAATTTTCTGCGCCAACCGGTGAAAGCATTGATTTTGCGC
TCCTATGGCGTGGGTAATGCGCCACAAAACAAAGCCTTCCTGCAGGAATTACATGAAGCCAGCGATCGCGGTATTGTGGT
GGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCG
TTATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTGAGCCAGGAACTGGATACTGAA
ACCATTCGCAAGGCCATGAGCCAAAACCTGCGCGGCGAACTGACGCCGGATGATTAA

Upstream 100 bases:

>100_bases
GCTGTCTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCTCCTGCCTCACGTATATACTTTTGCTCTTTCG
ATATCATTCATATCAATATC

Downstream 100 bases:

>100_bases
GGAGACTGCAATGCCCCCTCGCGCCCTGTTACTGGTCGATTTACAAAATGATTTCTGTGCTGGTGGCGCGCTCGCCGTGC
CGGAAGGTGACAGTACGGTG

Product: cytoplasmic asparaginase I

Products: NA

Alternate protein names: L-asparaginase I; L-ASNase I; L-asparagine amidohydrolase I [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRPEMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYD
DYDGFVILHGTDTMAYTASALSFMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIYPGISADVVRNFLRQPVKALILR
SYGVGNAPQNKAFLQELHEASDRGIVVVNLTQCMSGKVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTE
TIRKAMSQNLRGELTPDD

Sequences:

>Translated_338_residues
MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRPEMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYD
DYDGFVILHGTDTMAYTASALSFMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIYPGISADVVRNFLRQPVKALILR
SYGVGNAPQNKAFLQELHEASDRGIVVVNLTQCMSGKVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTE
TIRKAMSQNLRGELTPDD
>Mature_338_residues
MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRPEMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYD
DYDGFVILHGTDTMAYTASALSFMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIYPGISADVVRNFLRQPVKALILR
SYGVGNAPQNKAFLQELHEASDRGIVVVNLTQCMSGKVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTE
TIRKAMSQNLRGELTPDD

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=363, Percent_Identity=45.1790633608815, Blast_Score=279, Evalue=3e-75,
Organism=Escherichia coli, GI1788065, Length=338, Percent_Identity=99.7041420118343, Blast_Score=696, Evalue=0.0,
Organism=Escherichia coli, GI1789327, Length=282, Percent_Identity=28.0141843971631, Blast_Score=82, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI193207372, Length=361, Percent_Identity=44.8753462603878, Blast_Score=292, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI17558280, Length=358, Percent_Identity=45.2513966480447, Blast_Score=292, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI17558278, Length=358, Percent_Identity=45.2513966480447, Blast_Score=292, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI193207374, Length=358, Percent_Identity=45.2513966480447, Blast_Score=292, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24639609, Length=366, Percent_Identity=44.2622950819672, Blast_Score=287, Evalue=7e-78,
Organism=Drosophila melanogaster, GI24645530, Length=362, Percent_Identity=43.646408839779, Blast_Score=278, Evalue=3e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 37137; Mature: 37137

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRPEMPDFTIHEYTPLMDS
CCCCEEEEEEECCEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCC
SDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALSFMLENLGKPVIVTGSQIP
CCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCCC
LAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNRTTKAHADGFDAFASPNLPPL
HHHHCCCCCEEEEEEEEEEECCCCCEEEEEECCEEEECCCCCCCCCCCCCCCCCCCCCHH
LEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIYPGISADVVRNFLRQPVKALILR
HHCCEEEEECCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
SYGVGNAPQNKAFLQELHEASDRGIVVVNLTQCMSGKVNMGGYATGNALAHAGVIGGADM
HCCCCCCCCHHHHHHHHHHCCCCCEEEEEEHHHHCCCCCCCCEECCCHHHHCCEECCCCE
TVEATLTKLHYLLSQELDTETIRKAMSQNLRGELTPDD
EHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRPEMPDFTIHEYTPLMDS
CCCCEEEEEEECCEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCC
SDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALSFMLENLGKPVIVTGSQIP
CCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCCC
LAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNRTTKAHADGFDAFASPNLPPL
HHHHCCCCCEEEEEEEEEEECCCCCEEEEEECCEEEECCCCCCCCCCCCCCCCCCCCCHH
LEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIYPGISADVVRNFLRQPVKALILR
HHCCEEEEECCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
SYGVGNAPQNKAFLQELHEASDRGIVVVNLTQCMSGKVNMGGYATGNALAHAGVIGGADM
HCCCCCCCCHHHHHHHHHHCCCCCEEEEEEHHHHCCCCCCCCEECCCHHHHCCEECCCCE
TVEATLTKLHYLLSQELDTETIRKAMSQNLRGELTPDD
EHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]