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The map label for this gene is pncA

Identifier: 157161230

GI number: 157161230

Start: 1869969

End: 1870610

Strand: Direct

Name: pncA

Synonym: EcHS_A1852

Alternate gene names: 157161230

Gene position: 1869969-1870610 (Clockwise)

Preceding gene: 157161229

Following gene: 157161240

Centisome position: 40.27

GC content: 52.96

Gene sequence:

>642_bases
ATGCCCCCTCGCGCCCTGTTACTGGTCGATTTACAAAATGATTTCTGTGCTGGTGGCGCGCTCGCCGTGCCGGAAGGTGA
CAGTACGGTGGATGTCGCTAACCGCCTGATTGACTGGTGCCAGTCGCGCGGTGAAGCGGTTATCGCCAGTCAGGACTGGC
ACCCGGCGAATCACGGCAGTTTTGCCAGTCAGCACGGTGTAGAGCCTTATACGCCAGGCCAACTCGACGGTTTGCCACAA
ACCTTCTGGCCAGATCACTGTGTGCAGAACAGTGAAGGCGCACAATTACATCCGTTACTGCACCAAAAAGCGATCGCAGC
GGTGTTCCATAAAGGCGAAAATCCTTTAGTTGACAGTTACAGTGCCTTTTTTGATAACGGCCGTCGGCAGAAAACCTCTC
TCGATGACTGGTTACGCGATCATGAAATCGATGAATTGATCGTTATGGGCCTGGCTACTGACTATTGCGTGAAGTTTACC
GTGCTGGACGCGTTACAGTTAGGTTATAAGGTAAACGTGATTACCGATGGTTGTCGTGGCGTGAATATCCAGCCACAGGA
CAGTGCGCACGCGTTTATGGAGATGTCAGCTGCTGGGGCAACGCTATATACGCTGGCAGACTGGGAAGAGACACAGGGGT
AA

Upstream 100 bases:

>100_bases
CATTACCTGCTGAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCCAAAACCTGCGCGGCGAACTGACGCC
GGATGATTAAGGAGACTGCA

Downstream 100 bases:

>100_bases
TTTTACGCTGGCCTACAATTCTGTACTGGCATTGTAGGCCAAATAAAACACGTCAGTGGCACATCTGGCAATTGATGCCA
TCAACGAAAGATTTAATTCA

Product: nicotinamidase/pyrazinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRGEAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQ
TFWPDHCVQNSEGAQLHPLLHQKAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG

Sequences:

>Translated_213_residues
MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRGEAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQ
TFWPDHCVQNSEGAQLHPLLHQKAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG
>Mature_212_residues
PPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRGEAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQT
FWPDHCVQNSEGAQLHPLLHQKAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFTV
LDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family

Homologues:

Organism=Escherichia coli, GI87081970, Length=213, Percent_Identity=100, Blast_Score=444, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI193206735, Length=218, Percent_Identity=37.1559633027523, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17543130, Length=218, Percent_Identity=37.1559633027523, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71999339, Length=211, Percent_Identity=30.8056872037915, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321401, Length=196, Percent_Identity=33.1632653061224, Blast_Score=117, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24648247, Length=205, Percent_Identity=36.0975609756098, Blast_Score=117, Evalue=7e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PNCA_ECOLI (P21369)

Other databases:

- EMBL:   M26934
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   H64936
- RefSeq:   AP_002387.1
- RefSeq:   NP_416282.4
- ProteinModelPortal:   P21369
- SMR:   P21369
- DIP:   DIP-10520N
- STRING:   P21369
- EnsemblBacteria:   EBESCT00000003619
- EnsemblBacteria:   EBESCT00000017215
- GeneID:   946276
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1757
- KEGG:   eco:b1768
- EchoBASE:   EB1125
- EcoGene:   EG11135
- eggNOG:   COG1335
- GeneTree:   EBGT00050000011378
- HOGENOM:   HBG752434
- OMA:   HARLVIR
- ProtClustDB:   PRK11609
- BioCyc:   EcoCyc:NICOTINAMID-MONOMER
- BioCyc:   MetaCyc:NICOTINAMID-MONOMER
- Genevestigator:   P21369
- GO:   GO:0005829
- InterPro:   IPR000868
- Gene3D:   G3DSA:3.40.50.850

Pfam domain/function: PF00857 Isochorismatase; SSF52499 Iscrsm_hydrolase

EC number: =3.5.1.19

Molecular weight: Translated: 23362; Mature: 23231

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRGEAVIASQDWHPANHGS
CCCCEEEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCC
FASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLHQKAIAAVFHKGENPLVDSY
CHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCEEHHHHHHHHHHHHHHCCCCCCHHHH
SAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFTVLDALQLGYKVNVITDGCRG
HHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCC
VNIQPQDSAHAFMEMSAAGATLYTLADWEETQG
CCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCC
>Mature Secondary Structure 
PPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRGEAVIASQDWHPANHGS
CCCEEEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCC
FASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLHQKAIAAVFHKGENPLVDSY
CHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCEEHHHHHHHHHHHHHHCCCCCCHHHH
SAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFTVLDALQLGYKVNVITDGCRG
HHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCC
VNIQPQDSAHAFMEMSAAGATLYTLADWEETQG
CCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503