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The map label for this gene is rstA

Identifier: 157161069

GI number: 157161069

Start: 1704293

End: 1705012

Strand: Direct

Name: rstA

Synonym: EcHS_A1683

Alternate gene names: 157161069

Gene position: 1704293-1705012 (Clockwise)

Preceding gene: 157161067

Following gene: 157161070

Centisome position: 36.7

GC content: 49.17

Gene sequence:

>720_bases
ATGAACACTATCGTATTTGTGGAAGATGATGCGGAAGTCGGTTCACTGATTGCCGCGTACCTGGCAAAACATGATATGCA
GGTTACCGTAGAGCCGCGCGGCGACCAGGCCGAAGAAACCATTTTGCGAGAAAATCCGGATTTGGTGTTACTCGACATCA
TGCTACCAGGCAAAGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCT
CTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAAACGACGCCCCCTGCTGT
TTTGCTAGCGCGTTTACGTTTGCATTTGCGTCAGAATGAGCAAGCCACACTGACCAAAGGTCTTCAGGAAACGTCTCTGA
CTCCCTACAAAGCCCTGCATTTCGGCACGTTGACCATCGATCCCATCAACCGCGTAGTCACCCTGGCTAACACTGAAATC
TCGCTCTCGACAGCTGATTTCGAATTATTGTGGGAATTAGCTACCCATGCCGGGCAAATCATGGACCGCGATGCATTGCT
GAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACTAC
TCGATAACGCCGCAGAACCTTATCGCATTAAAACCGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAA

Upstream 100 bases:

>100_bases
TCATCAGTAGAATACCTGATGAAAACTTGTTTAGAAACGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGACGTTT
TTATATCTACCGTGAATGTT

Downstream 100 bases:

>100_bases
GCGATGAAAAAATTGTTTATCCAGTTTTACCTGTTATTGTTTGTCTGCTTCCTTGTGATGTCTCTGCTGGTTGGGCTGGT
GTACAAATTTACCGCCGAAC

Product: DNA-binding transcriptional regulator RstA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRENPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTS
LDSDMNHILALEMGACDYILKTTPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAAEPYRIKTVRNKGYLFAPHAWE

Sequences:

>Translated_239_residues
MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRENPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTS
LDSDMNHILALEMGACDYILKTTPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAAEPYRIKTVRNKGYLFAPHAWE
>Mature_239_residues
MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRENPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTS
LDSDMNHILALEMGACDYILKTTPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAAEPYRIKTVRNKGYLFAPHAWE

Specific function: Member of the two-component regulatory system rstB/rstA

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI145693140, Length=239, Percent_Identity=100, Blast_Score=488, Evalue=1e-139,
Organism=Escherichia coli, GI1789809, Length=233, Percent_Identity=34.3347639484979, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI87082012, Length=230, Percent_Identity=32.6086956521739, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1786784, Length=231, Percent_Identity=32.4675324675325, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1788394, Length=228, Percent_Identity=33.3333333333333, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI2367329, Length=233, Percent_Identity=33.0472103004292, Blast_Score=108, Evalue=2e-25,
Organism=Escherichia coli, GI1786599, Length=234, Percent_Identity=29.0598290598291, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI1790863, Length=232, Percent_Identity=28.448275862069, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786911, Length=234, Percent_Identity=28.6324786324786, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1789402, Length=230, Percent_Identity=30.8695652173913, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1790860, Length=231, Percent_Identity=27.7056277056277, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1787229, Length=232, Percent_Identity=29.3103448275862, Blast_Score=88, Evalue=4e-19,
Organism=Escherichia coli, GI1787375, Length=243, Percent_Identity=27.9835390946502, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790552, Length=236, Percent_Identity=28.3898305084746, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSTA_ECOLI (P52108)

Other databases:

- EMBL:   U41101
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   B64917
- RefSeq:   AP_002229.1
- RefSeq:   NP_416125.2
- ProteinModelPortal:   P52108
- SMR:   P52108
- DIP:   DIP-10807N
- MINT:   MINT-1274913
- STRING:   P52108
- EnsemblBacteria:   EBESCT00000001139
- EnsemblBacteria:   EBESCT00000016856
- GeneID:   946199
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1600
- KEGG:   eco:b1608
- EchoBASE:   EB2982
- EcoGene:   EG13190
- eggNOG:   COG0745
- GeneTree:   EBGT00070000031686
- HOGENOM:   HBG753323
- OMA:   GVSYDGM
- ProtClustDB:   PRK10701
- BioCyc:   EcoCyc:RSTA-MONOMER
- Genevestigator:   P52108
- GO:   GO:0005737
- GO:   GO:0006350
- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- SMART:   SM00448
- SMART:   SM00862

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C; SSF46894 Bipartite_resp_reg_C-effector; SSF52172 CheY_like

EC number: NA

Molecular weight: Translated: 26704; Mature: 26704

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRENPDLVLLDIMLPGKDG
CCEEEEEECCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHCCCCEEEEEEEECCCCC
MTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKTTPPAVLLARLRLHLRQNE
HHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCCCCEEECCCCHHHHHHHHHHHHCCCC
QATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEISLSTADFELLWELATHAGQI
HHHHHHCHHHCCCCCCHHEEEEEEEECCCCEEEEEECCEEEEEHHHHHHHHHHHHHHHHH
MDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAAEPYRIKTVRNKGYLFAPHAWE
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEECCCCCC
>Mature Secondary Structure
MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRENPDLVLLDIMLPGKDG
CCEEEEEECCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHCCCCEEEEEEEECCCCC
MTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKTTPPAVLLARLRLHLRQNE
HHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCCCCEEECCCCHHHHHHHHHHHHCCCC
QATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEISLSTADFELLWELATHAGQI
HHHHHHCHHHCCCCCCHHEEEEEEEECCCCEEEEEECCEEEEEHHHHHHHHHHHHHHHHH
MDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAAEPYRIKTVRNKGYLFAPHAWE
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1495392