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The map label for this gene is rstB [H]

Identifier: 157161070

GI number: 157161070

Start: 1705016

End: 1706317

Strand: Direct

Name: rstB [H]

Synonym: EcHS_A1684

Alternate gene names: 157161070

Gene position: 1705016-1706317 (Clockwise)

Preceding gene: 157161069

Following gene: 157161071

Centisome position: 36.72

GC content: 50.46

Gene sequence:

>1302_bases
ATGAAAAAATTGTTTATCCAGTTTTACCTGTTATTGTTTGTCTGCTTCCTTGTGATGTCTCTGCTGGTTGGGCTGGTGTA
CAAATTTACCGCCGAACGCGCGGGCAAACAGTCGCTGGATGATTTGATGAACAGTTCGCTGTATCTGATGCGCAGCGAAT
TGCGTGAGATCCCCCCACACGACTGGGGTAAGACGCTGAAAGAGATGGATTTAAATCTCTCTTTCGATCTGCGTGTCGAG
CCACTGAGTAAATACCATCTTGATGATATTTCCATGCACCGACTGCGTGGCGGCGAAATTGTCGCCCTGGACGATCAGTA
CACGTTTTTGCAGCGTATCCCGCGCAGCCACTACGTGCTGGCAGTTGGTCCTGTTCCTTATCTTTATTACCTCCATCAAA
TGCGATTGCTGGATATCGCCCTGATCGCTTTTATTGCTATTTCCCTCGCCTTTCCGGTGTTTATCTGGATGCGTCCGCAC
TGGCAGGATATGTTAAAACTGGAAGCAGCGGCGCAACGATTTGGCGATGGGCATCTCAATGAACGTATCCACTTTGATGA
GGGTTCGAGCTTTGAACGACTTGGCGTCGCATTTAACCAGATGGCGGACAATATCAACGCCTTAATTGCCAGCAAAAAAC
AGCTTATTGACGGTATCGCTCACGAACTGCGAACACCGTTAGTGCGCCTGCGTTATCGACTGGAGATGAGCGATAACCTG
AGCGCCGCCGAATCCCAGGCGTTGAATCGTGATATCAGTCAACTTGAAGCTTTAATTGAAGAGCTGCTGACTTATGCCCG
ACTCGATCGCCCACAAAACGAGCTTCATCTTAGCGAACCAGACCTGCCGTTGTGGCTGTCAACGCATCTGGCAGATATTC
AGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCATGCAAGGCCATTATGCGGCGTTGGATATGCGCTTAATG
GAGCGCGTGCTGGATAATTTGCTCAATAACGCCCTGCGCTACTGCCATTCAACGGTTGAAACCAGCCTGCTACTGTCGGG
GAATAAAGCGACATTAATTGTTGAGGATGATGGCTCAGGGATTGCCCCAGAAAACCGTGAACATATCTTTGAACCTTTTG
TTCGCCTCGATCCCAGCCGGGGTCGCTCAACCGGCGGCTGCGGGCTGGGACTGGCAATTGTCCACTCTATAGCACTGGCA
ATGGGCGGTACGGTTAATTGTGGCACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACAT
CCCGCAATTTACCTCTGCTTGA

Upstream 100 bases:

>100_bases
GTTAAGAAAAAAACTACTCGATAACGCCGCAGAACCTTATCGCATTAAAACCGTGCGTAACAAAGGCTATCTTTTTGCGC
CTCATGCATGGGAATAAGCG

Downstream 100 bases:

>100_bases
CACTACGCGCACGGTGGTCAAGTCACCACCACTGTGCTATAAATTAAGGATGTTGTAACTAAAGTGGTTAATATTATGGC
GCGTTACGATCTCGTAGACC

Product: sensor protein RstB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMNSSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVE
PLSKYHLDDISMHRLRGGEIVALDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDGIAHELRTPLVRLRYRLEMSDNL
SAAESQALNRDISQLEALIEELLTYARLDRPQNELHLSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLM
ERVLDNLLNNALRYCHSTVETSLLLSGNKATLIVEDDGSGIAPENREHIFEPFVRLDPSRGRSTGGCGLGLAIVHSIALA
MGGTVNCGTSELGGARFSFSWPLWHNIPQFTSA

Sequences:

>Translated_433_residues
MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMNSSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVE
PLSKYHLDDISMHRLRGGEIVALDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDGIAHELRTPLVRLRYRLEMSDNL
SAAESQALNRDISQLEALIEELLTYARLDRPQNELHLSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLM
ERVLDNLLNNALRYCHSTVETSLLLSGNKATLIVEDDGSGIAPENREHIFEPFVRLDPSRGRSTGGCGLGLAIVHSIALA
MGGTVNCGTSELGGARFSFSWPLWHNIPQFTSA
>Mature_433_residues
MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMNSSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVE
PLSKYHLDDISMHRLRGGEIVALDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDGIAHELRTPLVRLRYRLEMSDNL
SAAESQALNRDISQLEALIEELLTYARLDRPQNELHLSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLM
ERVLDNLLNNALRYCHSTVETSLLLSGNKATLIVEDDGSGIAPENREHIFEPFVRLDPSRGRSTGGCGLGLAIVHSIALA
MGGTVNCGTSELGGARFSFSWPLWHNIPQFTSA

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=433, Percent_Identity=98.8452655889146, Blast_Score=879, Evalue=0.0,
Organism=Escherichia coli, GI1789808, Length=265, Percent_Identity=32.0754716981132, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1790346, Length=272, Percent_Identity=33.8235294117647, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1786783, Length=311, Percent_Identity=29.903536977492, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1786600, Length=228, Percent_Identity=30.7017543859649, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1789403, Length=457, Percent_Identity=26.4770240700219, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788393, Length=269, Percent_Identity=23.7918215613383, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1787374, Length=283, Percent_Identity=26.8551236749117, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI87081816, Length=227, Percent_Identity=28.1938325991189, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI145693157, Length=254, Percent_Identity=23.2283464566929, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786912, Length=254, Percent_Identity=27.9527559055118, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI87082128, Length=248, Percent_Identity=25.8064516129032, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1788713, Length=234, Percent_Identity=25.6410256410256, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1788279, Length=278, Percent_Identity=28.4172661870504, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1789149, Length=227, Percent_Identity=27.3127753303965, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790436, Length=219, Percent_Identity=26.4840182648402, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 49162; Mature: 49162

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMNSSLYLMRSELREIPPH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHCCCC
DWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVALDDQYTFLQRIPRSHYVL
HHHHHHHHHCCCCEEEEEECCCHHHCCCCHHHHHCCCCCEEEECCCHHHHHHCCCCCEEE
AVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPHWQDMLKLEAAAQRFGDGHLN
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCC
ERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDGIAHELRTPLVRLRYRLEMSDNL
CCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SAAESQALNRDISQLEALIEELLTYARLDRPQNELHLSEPDLPLWLSTHLADIQAVTPDK
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHHEEECCCC
TVRIKTLMQGHYAALDMRLMERVLDNLLNNALRYCHSTVETSLLLSGNKATLIVEDDGSG
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCEEEEEEECCCCC
IAPENREHIFEPFVRLDPSRGRSTGGCGLGLAIVHSIALAMGGTVNCGTSELGGARFSFS
CCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEC
WPLWHNIPQFTSA
CCHHCCCCCCCCC
>Mature Secondary Structure
MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMNSSLYLMRSELREIPPH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHCCCC
DWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVALDDQYTFLQRIPRSHYVL
HHHHHHHHHCCCCEEEEEECCCHHHCCCCHHHHHCCCCCEEEECCCHHHHHHCCCCCEEE
AVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPHWQDMLKLEAAAQRFGDGHLN
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCC
ERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDGIAHELRTPLVRLRYRLEMSDNL
CCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SAAESQALNRDISQLEALIEELLTYARLDRPQNELHLSEPDLPLWLSTHLADIQAVTPDK
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHHEEECCCC
TVRIKTLMQGHYAALDMRLMERVLDNLLNNALRYCHSTVETSLLLSGNKATLIVEDDGSG
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCEEEEEEECCCCC
IAPENREHIFEPFVRLDPSRGRSTGGCGLGLAIVHSIALAMGGTVNCGTSELGGARFSFS
CCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEC
WPLWHNIPQFTSA
CCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]