BacMap

Definition	Escherichia coli HS, complete genome.
Accession	NC_009800
Length	4,643,538

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The map label for this gene is paaB

Identifier: 157160876

GI number: 157160876

Start: 1481457

End: 1482245

Strand: Direct

Name: paaB

Synonym: EcHS_A1481

Alternate gene names: 157160876

Gene position: 1481457-1482245 (Clockwise)

Preceding gene: 157160875

Following gene: 157160877

Centisome position: 31.9

GC content: 54.5

Gene sequence:

>789_bases
ATGATGGAATTCATCCTCAGTCATGTAGAAAAGGGCGTGATGACACTAACGCTCAACCGCCCGGAACGCCTGAACAGTTT
TAATGATGAGATGCACGCACAACTGGCAGAGTGCCTGAAACAGGTCGAGCGCGACGACACTATCCGTTGCCTGTTACTTA
CAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTA
GGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAA
TGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCACTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCA
TGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGCC
ATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGATCTGGCAGGTTGTTGATGA
TGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGTCTTGGACTTATCAAGC
AAGCGATAAATAGCGCTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGG
AGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAG

Upstream 100 bases:

>100_bases
AGGAGCGACAGTTATTCACCTTGCTGGCGGCAACAGAAGATCGTCATGAAGGCATCTCCGCTTTCTTACAAAAACGCACG
CCCGACTTTAAAGGACGCTA

Downstream 100 bases:

>100_bases
CATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCCGCCAGTC
ATGGACATCAGGTTTTACTG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVERDDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDL
GMSVERFYNPLVRRLAKLPKPVICAVNGVAAGAGATLALGGDIVIAARSAKFVMAFSKLGLIPDCGGTWLLPRVAGRARA
MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINSAETNTLDTQLDLERDYQRLAGR
SADYREGVSAFLAKRSPQFTGK

Sequences:

>Translated_262_residues
MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVERDDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDL
GMSVERFYNPLVRRLAKLPKPVICAVNGVAAGAGATLALGGDIVIAARSAKFVMAFSKLGLIPDCGGTWLLPRVAGRARA
MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINSAETNTLDTQLDLERDYQRLAGR
SADYREGVSAFLAKRSPQFTGK
>Mature_262_residues
MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVERDDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDL
GMSVERFYNPLVRRLAKLPKPVICAVNGVAAGAGATLALGGDIVIAARSAKFVMAFSKLGLIPDCGGTWLLPRVAGRARA
MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINSAETNTLDTQLDLERDYQRLAGR
SADYREGVSAFLAKRSPQFTGK

Specific function: Could possibly oxidize fatty acids using specific components

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family

Homologues:

Organism=Homo sapiens, GI194097323, Length=248, Percent_Identity=31.8548387096774, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI45643119, Length=257, Percent_Identity=28.4046692607004, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI260275230, Length=257, Percent_Identity=28.4046692607004, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI260274832, Length=257, Percent_Identity=28.4046692607004, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI4502327, Length=268, Percent_Identity=30.9701492537313, Blast_Score=95, Evalue=5e-20,
Organism=Homo sapiens, GI70995211, Length=208, Percent_Identity=29.8076923076923, Blast_Score=91, Evalue=9e-19,
Organism=Homo sapiens, GI213417737, Length=231, Percent_Identity=29.4372294372294, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI157694516, Length=231, Percent_Identity=29.4372294372294, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI37594469, Length=179, Percent_Identity=31.2849162011173, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI37594471, Length=181, Percent_Identity=31.4917127071823, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI150378541, Length=262, Percent_Identity=28.6259541984733, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI20127408, Length=189, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI4757968, Length=189, Percent_Identity=27.5132275132275, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI49355787, Length=189, Percent_Identity=27.5132275132275, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI31542718, Length=258, Percent_Identity=25.968992248062, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI221307494, Length=168, Percent_Identity=30.3571428571429, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI221136756, Length=187, Percent_Identity=28.8770053475936, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI221136753, Length=187, Percent_Identity=28.8770053475936, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI68989263, Length=218, Percent_Identity=27.9816513761468, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI25453481, Length=189, Percent_Identity=26.984126984127, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI51538573, Length=189, Percent_Identity=26.984126984127, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4757966, Length=189, Percent_Identity=26.984126984127, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI51538581, Length=189, Percent_Identity=26.984126984127, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI157694520, Length=206, Percent_Identity=28.1553398058252, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI116235487, Length=194, Percent_Identity=26.2886597938144, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787660, Length=262, Percent_Identity=100, Blast_Score=537, Evalue=1e-154,
Organism=Escherichia coli, GI1787659, Length=262, Percent_Identity=35.8778625954198, Blast_Score=144, Evalue=4e-36,
Organism=Escherichia coli, GI221142681, Length=261, Percent_Identity=29.8850574712644, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI87082183, Length=257, Percent_Identity=28.7937743190661, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788597, Length=257, Percent_Identity=29.5719844357977, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790281, Length=195, Percent_Identity=29.2307692307692, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788682, Length=200, Percent_Identity=29.5, Blast_Score=76, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25145438, Length=250, Percent_Identity=34.4, Blast_Score=137, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI17554946, Length=253, Percent_Identity=30.4347826086957, Blast_Score=130, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17560910, Length=203, Percent_Identity=28.5714285714286, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17549921, Length=232, Percent_Identity=26.7241379310345, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17540714, Length=231, Percent_Identity=29.8701298701299, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17535521, Length=248, Percent_Identity=25.8064516129032, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17534483, Length=257, Percent_Identity=24.9027237354086, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17536985, Length=260, Percent_Identity=24.6153846153846, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17540306, Length=256, Percent_Identity=26.5625, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25144157, Length=196, Percent_Identity=29.0816326530612, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI25144160, Length=196, Percent_Identity=29.0816326530612, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI25141363, Length=190, Percent_Identity=27.3684210526316, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6320241, Length=227, Percent_Identity=25.9911894273128, Blast_Score=86, Evalue=6e-18,
Organism=Drosophila melanogaster, GI20129971, Length=261, Percent_Identity=36.0153256704981, Blast_Score=145, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24653477, Length=261, Percent_Identity=36.0153256704981, Blast_Score=145, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24653139, Length=262, Percent_Identity=31.2977099236641, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI19920382, Length=252, Percent_Identity=28.5714285714286, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI19922422, Length=263, Percent_Identity=25.0950570342205, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21357171, Length=223, Percent_Identity=29.5964125560538, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24654903, Length=196, Percent_Identity=26.530612244898, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI28571729, Length=178, Percent_Identity=28.6516853932584, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI28571730, Length=178, Percent_Identity=28.6516853932584, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24650670, Length=212, Percent_Identity=27.3584905660377, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PAAG_ECOLI (P77467)

Other databases:

- EMBL:   X97452
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   E64890
- RefSeq:   AP_002021.1
- RefSeq:   NP_415912.1
- ProteinModelPortal:   P77467
- SMR:   P77467
- IntAct:   P77467
- STRING:   P77467
- PRIDE:   P77467
- EnsemblBacteria:   EBESCT00000002468
- EnsemblBacteria:   EBESCT00000017727
- GeneID:   946263
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1389
- KEGG:   eco:b1394
- EchoBASE:   EB3504
- EcoGene:   EG13741
- eggNOG:   COG1024
- GeneTree:   EBGT00050000009097
- HOGENOM:   HBG748731
- OMA:   RSDDYRE
- ProtClustDB:   PRK08140
- BioCyc:   EcoCyc:G6715-MONOMER
- BioCyc:   MetaCyc:G6715-MONOMER
- Genevestigator:   P77467
- InterPro:   IPR001753
- InterPro:   IPR011968
- TIGRFAMs:   TIGR02280

Pfam domain/function: PF00378 ECH

EC number: =4.2.1.17

Molecular weight: Translated: 28405; Mature: 28405

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVERDDTIRCLLLTGAGRGF
CHHHHHHHHHCCEEEEEECCCHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
CAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPVICAVNGVAAGAGATLALG
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEC
GDIVIAARSAKFVMAFSKLGLIPDCGGTWLLPRVAGRARAMGLALLGNQLSAEQAHEWGM
CCEEEEECCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHCHHHHHHCCC
IWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINSAETNTLDTQLDLERDYQRLAGR
EEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCC
SADYREGVSAFLAKRSPQFTGK
CCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVERDDTIRCLLLTGAGRGF
CHHHHHHHHHCCEEEEEECCCHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
CAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPVICAVNGVAAGAGATLALG
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEC
GDIVIAARSAKFVMAFSKLGLIPDCGGTWLLPRVAGRARAMGLALLGNQLSAEQAHEWGM
CCEEEEECCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHCHHHHHHCCC
IWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINSAETNTLDTQLDLERDYQRLAGR
EEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCC
SADYREGVSAFLAKRSPQFTGK
CCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858