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The map label for this gene is uspF [H]

Identifier: 157160857

GI number: 157160857

Start: 1458377

End: 1458811

Strand: Reverse

Name: uspF [H]

Synonym: EcHS_A1462

Alternate gene names: 157160857

Gene position: 1458811-1458377 (Counterclockwise)

Preceding gene: 157160858

Following gene: 157160856

Centisome position: 31.42

GC content: 48.51

Gene sequence:

>435_bases
ATGAACAGAACGATTCTTGTCCCTATCGATATTTCCGATTCAGAATTAACTCAACGCGTGATTAGCCACGTTGAGGAAGA
GGCAAAGATTGATGATGCAGAGGTTCATTTCCTGACGGTAATACCTTCACTGCCCTACTATGCCTCTCTGGGTTTAGCGT
ATTCCGCAGAATTACCGGCAATGGATGACCTGAAAGCGGAAGCCAAATCGCAACTGGAAGAGATCATTAAAAAATTTAAA
CTGCCAACCGACAGAGTGCATGTCCATGTTGAGGAAGGCTCGCCCAAAGACCGCATTCTGGAATTGGCGAAGAAGATCCC
CGCTCATATGATCATCATTGCTTCCCATCGACCGGATATCACCACTTATCTGCTCGGTTCCAACGCCGCAGCTGTAGTGC
GTCACGCAGAGTGCTCCGTGCTGGTTGTGCGCTGA

Upstream 100 bases:

>100_bases
TCTTCCTCCTTTGATCTAAATTAAAAATGTGAACTCCGTCATTACACAAAAAGTGTCATCTGGCGTTACACTTTATGCGG
ATACTAAAACAGGAGGTTTT

Downstream 100 bases:

>100_bases
CACTAACGCCCGCACATTGCTGCGGGCTTTTTGATTCATTTCGCAAATGTGCTGACATTTTCCCCTCTAATCCGTACCAT
ACACGCCACAGTTTTTTATA

Product: universal stress protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 144; Mature: 144

Protein sequence:

>144_residues
MNRTILVPIDISDSELTQRVISHVEEEAKIDDAEVHFLTVIPSLPYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFK
LPTDRVHVHVEEGSPKDRILELAKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR

Sequences:

>Translated_144_residues
MNRTILVPIDISDSELTQRVISHVEEEAKIDDAEVHFLTVIPSLPYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFK
LPTDRVHVHVEEGSPKDRILELAKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR
>Mature_144_residues
MNRTILVPIDISDSELTQRVISHVEEEAKIDDAEVHFLTVIPSLPYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFK
LPTDRVHVHVEEGSPKDRILELAKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR

Specific function: Unknown

COG id: COG0589

COG function: function code T; Universal stress protein UspA and related nucleotide-binding proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the universal stress protein A family [H]

Homologues:

Organism=Escherichia coli, GI87081893, Length=144, Percent_Identity=100, Blast_Score=288, Evalue=1e-79,
Organism=Escherichia coli, GI1786824, Length=146, Percent_Identity=39.041095890411, Blast_Score=100, Evalue=6e-23,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014729
- InterPro:   IPR006015
- InterPro:   IPR006016 [H]

Pfam domain/function: PF00582 Usp [H]

EC number: NA

Molecular weight: Translated: 16017; Mature: 16017

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRTILVPIDISDSELTQRVISHVEEEAKIDDAEVHFLTVIPSLPYYASLGLAYSAELPA
CCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHCCEEEECCCCC
MDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILELAKKIPAHMIIIASHRPDI
CHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCE
TTYLLGSNAAAVVRHAECSVLVVR
EEEEECCCCEEEEEECCEEEEEEC
>Mature Secondary Structure
MNRTILVPIDISDSELTQRVISHVEEEAKIDDAEVHFLTVIPSLPYYASLGLAYSAELPA
CCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHCCEEEECCCCC
MDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILELAKKIPAHMIIIASHRPDI
CHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCE
TTYLLGSNAAAVVRHAECSVLVVR
EEEEECCCCEEEEEECCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]