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The map label for this gene is ttcA [H]

Identifier: 157160856

GI number: 157160856

Start: 1457265

End: 1458200

Strand: Reverse

Name: ttcA [H]

Synonym: EcHS_A1461

Alternate gene names: 157160856

Gene position: 1458200-1457265 (Counterclockwise)

Preceding gene: 157160857

Following gene: 157160853

Centisome position: 31.4

GC content: 48.82

Gene sequence:

>936_bases
ATGTCGCAAAATCAAGAAATTAGTAAGAAAGAACAATACAACCTGAACAAATTACAAAAACGTCTGCGTCGTAACGTGGG
CGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGTAAAGACAGCTATA
CCATGCTGGAGATTCTGCGCAATTTGCAGCAAAGCGCGCCAATCAATTTTTCGCTGGTGGCTGTTAACCTCGATCAAAAG
CAACCGGGCTTCCCGGAACACGTTCTGCCCGAGTATCTTGAAAAGCTGGGCGTTGAGTACAAGATTGTTGAAGAGAATAC
TTACGGTATCGTGAAAGAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGCCTTCGTCGCGGTATCCTTT
ATCGTACCGCAACGGAACTGGGGGCGACGAAGATCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTA
AATATGTTCTACGGCGGTAAGATGAAAGGTATGCCTCCGAAACTGATGAGCGATGATGGCAAACATATCGTTATTCGTCC
GCTGGCCTACTGCCGCGAGAAAGATATTCAGCGATTTGCCGATGCAAAAGCGTTCCCGATTATTCCGTGCAACCTGTGCG
GTTCACAGCCTAACCTGCAACGTCAGGTGATTGCTGACATGTTGCGTGACTGGGATAAACGTTATCCAGGGCGTATCGAG
ACGATGTTCAGCGCGATGCAGAATGTGGTGCCGTCGCATCTGTGCGATACCAACCTGTTCGATTTCAAAGGCATCACCCA
CGGTTCTGAAGTGGTTAACGGGGGTGATCTGGCGTTTGATCGCGAAGAGATCCCACTACAACCGGCGGGCTGGCAGCCAG
AAGAAGATGAAAATCAGTTGGATGAGTTACGGCTGAATGTGGTTGAAGTGAAATAA

Upstream 100 bases:

>100_bases
CCATACACGCCACAGTTTTTTATATCAGATTTCTTATGCTGGGCGTTCCGGCATGACGCATACTCTTCTGATGCCATATA
ACGAATTGAGTCGCTTTTAA

Downstream 100 bases:

>100_bases
CCAGGATAGCGCCCGATGCGCAAGCGTATCGGGCTACTCTTATGGAGGCCGGATAAGACGCGGCCAGCGTCGCATCCGGC
AATCCCGAATAAGATGTTTA

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMVCLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQK
QPGFPEHVLPEYLEKLGVEYKIVEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPNLQRQVIADMLRDWDKRYPGRIE
TMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGDLAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK

Sequences:

>Translated_311_residues
MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMVCLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQK
QPGFPEHVLPEYLEKLGVEYKIVEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPNLQRQVIADMLRDWDKRYPGRIE
TMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGDLAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK
>Mature_310_residues
SQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMVCLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQ
PGFPEHVLPEYLEKLGVEYKIVEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFLN
MFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPNLQRQVIADMLRDWDKRYPGRIET
MFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGDLAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32) [H]

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family [H]

Homologues:

Organism=Escherichia coli, GI1787606, Length=311, Percent_Identity=98.3922829581994, Blast_Score=639, Evalue=0.0,
Organism=Caenorhabditis elegans, GI193211090, Length=260, Percent_Identity=30.7692307692308, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17540090, Length=235, Percent_Identity=27.2340425531915, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24651972, Length=234, Percent_Identity=26.9230769230769, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 35460; Mature: 35329

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMVCLSGGKDSYTMLEILR
CCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHH
NLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKIVEENTYGIVKEKIPEGKT
HHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHCCEEEEEECCCCHHHHHHCCCCCH
TCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFLNMFYGGKMKGMPPKLMSDDG
HHHHHHHHHHHHHHHHHHHHCCHHEECCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCC
KHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPNLQRQVIADMLRDWDKRYPGRIE
CEEEECCHHHHHHHHHHHHHCCCCCCEEEHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHH
TMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGDLAFDREEIPLQPAGWQPEEDENQL
HHHHHHHHHHHHHHCCCCCHHCCCCCCCHHHCCCCCCEECCCCCCCCCCCCCCCCHHHHH
DELRLNVVEVK
HHHHHEEEECC
>Mature Secondary Structure 
SQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMVCLSGGKDSYTMLEILR
CCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHH
NLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKIVEENTYGIVKEKIPEGKT
HHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHCCEEEEEECCCCHHHHHHCCCCCH
TCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFLNMFYGGKMKGMPPKLMSDDG
HHHHHHHHHHHHHHHHHHHHCCHHEECCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCC
KHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPNLQRQVIADMLRDWDKRYPGRIE
CEEEECCHHHHHHHHHHHHHCCCCCCEEEHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHH
TMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGDLAFDREEIPLQPAGWQPEEDENQL
HHHHHHHHHHHHHHCCCCCHHCCCCCCCHHHCCCCCCEECCCCCCCCCCCCCCCCHHHHH
DELRLNVVEVK
HHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA