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The map label for this gene is ydaM [H]

Identifier: 157160853

GI number: 157160853

Start: 1452815

End: 1454107

Strand: Reverse

Name: ydaM [H]

Synonym: EcHS_A1458

Alternate gene names: 157160853

Gene position: 1454107-1452815 (Counterclockwise)

Preceding gene: 157160856

Following gene: 157160850

Centisome position: 31.31

GC content: 47.25

Gene sequence:

>1293_bases
ATGACGTGGCTTTTTTGCGATCGGATAGCAACAAAAATTGATAAAAATAACGGGATCTCAATGATTACGCACAACTTCAA
TACCCTGGACTTACTCACCAGTCCTGTCTGGATCGTTTCGCCCTTTGAGGAACAGTTAATTTATGCCAATAGCGCGGCGA
AACTGTTGATGCAAGACCTCACGTTTAGTCAGCTACGAACCGGACCCTATTCCGTCTCCTCACAAAAAGAACTGCCGAAA
TACCTCTCCGATCTGCAAAACCAACACGATATTATCGAAATCCTCACTGTTCAGCGTAAAGAAGAGGAAACAGCATTGAG
CTGTCGGCTTGTTTTGCGAAAGCTGACAGAAACAGAACCGGTGATTATTTTCGAAGGTATCGAAGCGCCGGCAACGCTGG
GTTTAAAAGCCAGTCGCTCGGCAAATTATCAGCGCAAAAAACAAGGTTTTTATGCGCGCTTTTTTCTGACTAACTCTGCA
CCAATGTTGTTGATTGACCCGTCACGAGATGGACAAATCGTCGATGCTAACCTCGCCGCGCTCAATTTCTATGGTTATAA
CCATGAAACGATGTGCCAGAAACATACCTGGGAAATAAATATGCTCGGGCGTCGCGTCATGCCTATCATGCATGAAATCT
CGCATTTACCCGGTGGTCATAAGCCTTTGAATTTTGTTCATAAACTGGCGGATGGTTCGACTCGTCATGTGCAGACCTAT
GCCGGACCGATTGAAATTTATGGCGACAAGCTCATGTTATGTATTGTGCATGATATTACTGAGCAAAAACGGCTGGAGGA
GCAGCTGGAACATGCTGCTCACCATGACGCGATGACCGGATTACTGAATCGGCGACAGTTTTATCACATTACGGAACCAG
GCCAAATGCAGCATCTCGCCATCGCTCAGGATTACAGCTTGTTGCTCATCGACACCGATCGTTTTAAACACATTAACGAT
CTCTATGGGCATTCTAAAGGTGATGAGGTGTTATGCGCCCTCGCCCGCACCCTCGAAAGTTGCGCTCGCAAAGGCGATTT
GGTGTTTCGTTGGGGAGGCGAAGAGTTTGTCTTATTGCTACCAAGAACCCCACTGGATACCGCGCTTTCGCTGGCTGAAA
CTATCCGCGTAAGCGTGGCAAAAGTGAGTATTTCGGGCTTACCACGCTTTACCGTCAGCATTGGTGTGGCGCATCACGAA
GGAAATGAAAGCATCGATGAACTGTTTAAACGCGTTGATGATGCTTTGTATCGGGCGAAAAATGATGGACGCAACCGCGT
GCTGGCGGCATAA

Upstream 100 bases:

>100_bases
AATTCTGTCTAAATTGAATACAAAAAGCAAAATGCTTTTCCGTATACAAACCGTGTGAAGTGTTAAATAGCGTCTATCAT
TATCAGAATTATCTGATCAT

Downstream 100 bases:

>100_bases
GCCGCGGATGCGTCTCGAGATCAACGACTGCGCTTAGCGTGGCGCTCCCAGTTTTCTTGCTTCGCCTGCGCCGTTTTACG
TAGTGCGACGTAACACGCCC

Product: sensory box-containing diguanylate cyclase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MTWLFCDRIATKIDKNNGISMITHNFNTLDLLTSPVWIVSPFEEQLIYANSAAKLLMQDLTFSQLRTGPYSVSSQKELPK
YLSDLQNQHDIIEILTVQRKEEETALSCRLVLRKLTETEPVIIFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSA
PMLLIDPSRDGQIVDANLAALNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQTY
AGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQMQHLAIAQDYSLLLIDTDRFKHIND
LYGHSKGDEVLCALARTLESCARKGDLVFRWGGEEFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHE
GNESIDELFKRVDDALYRAKNDGRNRVLAA

Sequences:

>Translated_430_residues
MTWLFCDRIATKIDKNNGISMITHNFNTLDLLTSPVWIVSPFEEQLIYANSAAKLLMQDLTFSQLRTGPYSVSSQKELPK
YLSDLQNQHDIIEILTVQRKEEETALSCRLVLRKLTETEPVIIFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSA
PMLLIDPSRDGQIVDANLAALNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQTY
AGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQMQHLAIAQDYSLLLIDTDRFKHIND
LYGHSKGDEVLCALARTLESCARKGDLVFRWGGEEFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHE
GNESIDELFKRVDDALYRAKNDGRNRVLAA
>Mature_429_residues
TWLFCDRIATKIDKNNGISMITHNFNTLDLLTSPVWIVSPFEEQLIYANSAAKLLMQDLTFSQLRTGPYSVSSQKELPKY
LSDLQNQHDIIEILTVQRKEEETALSCRLVLRKLTETEPVIIFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAP
MLLIDPSRDGQIVDANLAALNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQTYA
GPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQMQHLAIAQDYSLLLIDTDRFKHINDL
YGHSKGDEVLCALARTLESCARKGDLVFRWGGEEFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEG
NESIDELFKRVDDALYRAKNDGRNRVLAA

Specific function: Unknown

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=410, Percent_Identity=100, Blast_Score=854, Evalue=0.0,
Organism=Escherichia coli, GI145693134, Length=167, Percent_Identity=36.5269461077844, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786584, Length=171, Percent_Identity=35.0877192982456, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI87082007, Length=172, Percent_Identity=35.4651162790698, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788381, Length=179, Percent_Identity=34.0782122905028, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787262, Length=166, Percent_Identity=34.3373493975904, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787802, Length=174, Percent_Identity=36.2068965517241, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788085, Length=182, Percent_Identity=29.1208791208791, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787816, Length=160, Percent_Identity=35.625, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787541, Length=200, Percent_Identity=29, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787056, Length=163, Percent_Identity=32.5153374233129, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87081974, Length=163, Percent_Identity=28.8343558282209, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1788956, Length=162, Percent_Identity=32.0987654320988, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013656 [H]

Pfam domain/function: PF00990 GGDEF; PF08448 PAS_4 [H]

EC number: NA

Molecular weight: Translated: 48762; Mature: 48630

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS50113 PAC ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTWLFCDRIATKIDKNNGISMITHNFNTLDLLTSPVWIVSPFEEQLIYANSAAKLLMQDL
CCEEEHHHHHHHHCCCCCEEEEEECCCCHHHHCCCEEEECCHHHHEEECCHHHHHHHHHH
TFSQLRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRKLTETEP
HHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCC
VIIFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
EEEEECCCCCCCCCEECCCCCCHHHHHCCEEEEEEEECCCCEEEECCCCCCEEEEEEEEE
LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQTY
EEEECCCCHHHHHHCCEEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEEEE
AGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQMQHLA
CCCEEEECCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEE
IAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGEEFVLLL
EECCCEEEEEECHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEE
PRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRVDDALYRAK
CCCCHHHHHHHHHHHHHEEEEEEECCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHC
NDGRNRVLAA
CCCCCCCCCC
>Mature Secondary Structure 
TWLFCDRIATKIDKNNGISMITHNFNTLDLLTSPVWIVSPFEEQLIYANSAAKLLMQDL
CEEEHHHHHHHHCCCCCEEEEEECCCCHHHHCCCEEEECCHHHHEEECCHHHHHHHHHH
TFSQLRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRKLTETEP
HHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCC
VIIFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
EEEEECCCCCCCCCEECCCCCCHHHHHCCEEEEEEEECCCCEEEECCCCCCEEEEEEEEE
LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQTY
EEEECCCCHHHHHHCCEEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEEEE
AGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQMQHLA
CCCEEEECCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEE
IAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGEEFVLLL
EECCCEEEEEECHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEE
PRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRVDDALYRAK
CCCCHHHHHHHHHHHHHEEEEEEECCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHC
NDGRNRVLAA
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]