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The map label for this gene is pgmB

Identifier: 157160828

GI number: 157160828

Start: 1427598

End: 1428257

Strand: Direct

Name: pgmB

Synonym: EcHS_A1432

Alternate gene names: 157160828

Gene position: 1427598-1428257 (Clockwise)

Preceding gene: 157160827

Following gene: 157160829

Centisome position: 30.74

GC content: 56.52

Gene sequence:

>660_bases
ATGAAACTGCAAGGGGTAATTTTCGATCTGGATGGTGTAATCACCGATACCGCGCATCTGCATTTCCAGGCGTGGCAGCA
GATTGCCGCTGAAATTGGCATCAGCATTGATGCGCAGTTTAACGAATCCCTAAAAGGGATCAGCCGCGATGAGTCTCTGC
GGCGCATTCTGCAACACGGGGGCAAAGAGGGCGACTTTAACTCGCAGGAGAGGGCGCAACTGGCGTATCGCAAAAATCTG
CTCTATGTCCACTCACTACGCGAGTTGACGGTCAACGCTGTTCTACCCGGCATTCGCTCTTTGCTGGCAGATCTCCGTGC
ACAGCAGATCTCGGTTGGGCTGGCTTCTGTCTCCCTGAATGCGCCGACGATTTTAGCGGCGCTGGAGCTGCGCGAGTTTT
TCACCTTCTGCGCGGATGCTTCCCAACTTAAAAACTCGAAACCGGACCCGGAAATCTTTCTCGCCGCCTGTGCAGGGCTG
GGCGTGCCGCCGCAGGCATGTATCGGCATTGAAGATGCGCAGGCGGGCATTGACGCCATTAACGCCAGCGGTATGCGCTC
GGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGG
CCTTCTGGCAAAACGTATAG

Upstream 100 bases:

>100_bases
TGAACGGTCAGCTTATAACCGTGGCTGAAGAATCTGTTTTCTGTTTGGGTGATTTTATTTTGCCCTTCAATGGGACCGCT
ACCAAACATCAGGGGGATGA

Downstream 100 bases:

>100_bases
CAAAGGAATCAACATGGCTCAGTTTTCGTTACAACATATTCAAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACT
TCAACCTGGAAATTGCCGAT

Product: putative beta-phosphoglucomutase

Products: NA

Alternate protein names: Beta-PGM

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESLKGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNL
LYVHSLRELTVNAVLPGIRSLLADLRAQQISVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTESLTWPRLSAFWQNV

Sequences:

>Translated_219_residues
MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESLKGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNL
LYVHSLRELTVNAVLPGIRSLLADLRAQQISVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTESLTWPRLSAFWQNV
>Mature_219_residues
MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESLKGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNL
LYVHSLRELTVNAVLPGIRSLLADLRAQQISVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTESLTWPRLSAFWQNV

Specific function: Reversible transformation of glucose 6-phosphate and beta-glucose 1-phosphate

COG id: COG0637

COG function: function code R; Predicted phosphatase/phosphohexomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family

Homologues:

Organism=Homo sapiens, GI197382691, Length=202, Percent_Identity=28.7128712871287, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787576, Length=219, Percent_Identity=100, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI1789046, Length=191, Percent_Identity=29.3193717277487, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1788021, Length=189, Percent_Identity=31.2169312169312, Blast_Score=77, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17137324, Length=197, Percent_Identity=27.9187817258883, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI116008157, Length=200, Percent_Identity=27, Blast_Score=67, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PGMB_ECOLI (P77366)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- PIR:   H64880
- RefSeq:   AP_001943.1
- RefSeq:   NP_415833.1
- ProteinModelPortal:   P77366
- SMR:   P77366
- STRING:   P77366
- EnsemblBacteria:   EBESCT00000000872
- EnsemblBacteria:   EBESCT00000015701
- GeneID:   945891
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1310
- KEGG:   eco:b1317
- EchoBASE:   EB3677
- EcoGene:   EG13918
- eggNOG:   COG0637
- GeneTree:   EBGT00050000009691
- HOGENOM:   HBG742904
- OMA:   YIVDANE
- ProtClustDB:   CLSK880111
- BioCyc:   EcoCyc:G6655-MONOMER
- BioCyc:   MetaCyc:G6655-MONOMER
- Genevestigator:   P77366
- InterPro:   IPR010976
- InterPro:   IPR010972
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006402
- InterPro:   IPR005833
- Gene3D:   G3DSA:3.40.50.1000
- PRINTS:   PR00413
- TIGRFAMs:   TIGR01990
- TIGRFAMs:   TIGR01509
- TIGRFAMs:   TIGR02009

Pfam domain/function: PF00702 Hydrolase; SSF56784 SSF56784

EC number: =5.4.2.6

Molecular weight: Translated: 23565; Mature: 23565

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESLKGISRDESLRRILQHG
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHHCC
GKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLLADLRAQQISVGLASVSLN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECC
APTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGLGVPPQACIGIEDAQAGIDAI
CCHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHCCCCCHHHHCCCCHHHHCHHHH
NASGMRSVGIGAGLTGAQLLLPSTESLTWPRLSAFWQNV
CCCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHCCC
>Mature Secondary Structure
MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESLKGISRDESLRRILQHG
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHHCC
GKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLLADLRAQQISVGLASVSLN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECC
APTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGLGVPPQACIGIEDAQAGIDAI
CCHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHCCCCCHHHHCCCCHHHHCHHHH
NASGMRSVGIGAGLTGAQLLLPSTESLTWPRLSAFWQNV
CCCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503