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The map label for this gene is ycjT [H]

Identifier: 157160827

GI number: 157160827

Start: 1425334

End: 1427601

Strand: Direct

Name: ycjT [H]

Synonym: EcHS_A1431

Alternate gene names: 157160827

Gene position: 1425334-1427601 (Clockwise)

Preceding gene: 157160826

Following gene: 157160828

Centisome position: 30.7

GC content: 53.75

Gene sequence:

>2268_bases
ATGACCAGGCCAGTAACGTTATCAGAACCCCATTTCAGCCAGCATACCCTGAACAAGTATGCATCGCTGATGGCGCAGGG
GAACGGCTATCTTGGGCTTCGCGCCAGCCATGAAGAAGATTACACGCGCCAGACGCGAGGGATGTATCTGGCGGGGCTGT
ATCATCGGGCGGGAAAAGGTGAAATCAACGAACTGGTGAACCTGCCTGATGTCGTGGGGATGGAGATTGCCATAAATGGT
GAGGTTTTCTCGTTATCCCACGAAGCCTGGCAGCGTGAGCTTGACTTTGCCAGTGGCGAATTACGCCGCAATGTTGTCTG
GCGTACCAGCAACGGCTCAGGTTACACCATCGCCAGCCGTCGCTTTGTTTCGGCAGACCAACTACCGCTCATTGCGCTGG
AAATCACTATTACGCCACTGGACGCCGACGCGTCAGTGCTGATTTCAACAGGCATTGACGCCACGCAAACCAATCACGGT
CGCCAACATCTCGACGAAACCCAGGTGCGGGTGTTTGGTCAGCATCTGATGCAGGGGAGCTACACCACCCAGGATGGACG
CAGTGATGTGGCCATCAGCTGTTGCTGTAAGGTGAGCGGTGATGTGCAGCAATGCTATACCGCCAAAGAGCGCCGTTTAC
TGCAACATACCAGTGCGCAGCTTCATGCAGGCGAGACAATGACGTTGCAAAAACTGGTGTGGATCGACTGGCGGGATGAC
AGGCAAGCTGCTTTAGACGAGTGGGGCAGCGCGTCGCTTCGCCAGCTTGAAATGTGCGCGCAGCAGAGTTACGACCAACT
TCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGCGGCGAAGCGCACGATC
AGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAGCATTGCGGCAAAA
GGCTTAACCGGCGAAGGCTACAAAGGCCACGTTTTCTGGGATACAGAAGTATTTTTGTTACCGTTTCATCTGTTTAGCGA
TCCGACGGTTGCCCGAAGTTTACTGCGTTATCGCTGGCACAACTTGCCAGGCGCGCAGGAGAAAGCGCGACGCAACGGCT
GGCAGGGCGCGCTATTTCCGTGGGAAAGCGCGCGCAGCGGCGAAGAAGAGACGCCGGAATTTGCCGCCATTAACATTCGC
ACCGGGCTGCGGCAAAAAGTGGCCTCGGCGCAGGCGGAACATCATCTGGTGGCCGATATCGCCTGGGCGGTTATTCAATA
CTGGCAGACCACGGGGGATGAAAGTTTCATTGCGCATGAAGGCATGGCGCTACTTCTGGAGACGGCAAAGTTCTGGATTA
GCCGCGCGGTGAGAGTTAACGATCGTCTGGAAATTCATGATGTTATTGGGCCAGACGAATATACCGAACATGTCAATAAT
AATGCATACACCAGCTATATGGCCCGCTACAACGTTCAACAGGCGCTGAATATTGCCCGCCAGTTCGGCTGTAGCGACGA
TGCGTTTATCCATCGCGCCGAAATGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGG
ATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACTGCTGGAT
TATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCA
GTTCTCAGCGGCATCGTGTCTTGCCAATCTGCAATTTTATGAACCGCGCACTATTCACGACTCGTCATTAAGTAAAGCAA
TCCACGGCATTGTTGCCGCACGCTGTGGCCTGCTGACCCAAAGTTATCAGTTCTGGCGCGAGGGGACTGAAATCGATCTT
GGTGCTGATCCGCATAGTTGTGATGATGGTATCCATGCTGCCGCAACTGGCGCTATCTGGCTGGGGGCGATTCAGGGTTT
TGCCGGGGTGAGCGTGCGTGACGGTGAATTGCATCTCAATCCGGCGTTACCTGAGCAGTGGCAACAGTTGTCTTTCCCTC
TGTTCTGGCAGGGCTGCGAATTACAGGTCACTCTTGACGCGCAGCGTATTGCGATTCGAACTTCTGCGCCCGTTTCACTG
CGTTTGAACGGTCAGCTTATAACCGTGGCTGAAGAATCTGTTTTCTGTTTGGGTGATTTTATTTTGCCCTTCAATGGGAC
CGCTACCAAACATCAGGGGGATGAATGA

Upstream 100 bases:

>100_bases
GCAAGCCCGTGATGATAGCCGACGCCGAGCAGGGGTACATCATCCAGCAACTGGTGGCGGCGTTGTATCAATCCGCAGAA
ACAGGGACGCGTGTGGAATT

Downstream 100 bases:

>100_bases
AACTGCAAGGGGTAATTTTCGATCTGGATGGTGTAATCACCGATACCGCGCATCTGCATTTCCAGGCGTGGCAGCAGATT
GCCGCTGAAATTGGCATCAG

Product: glycosyl hydrolase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 755; Mature: 754

Protein sequence:

>755_residues
MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQTRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAING
EVFSLSHEAWQRELDFASGELRRNVVWRTSNGSGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
RQHLDETQVRVFGQHLMQGSYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTSAQLHAGETMTLQKLVWIDWRDD
RQAALDEWGSASLRQLEMCAQQSYDQLLAASTENWRQWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAK
GLTGEGYKGHVFWDTEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETPEFAAINIR
TGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAKFWISRAVRVNDRLEIHDVIGPDEYTEHVNN
NAYTSYMARYNVQQALNIARQFGCSDDAFIHRAEMFLKELWMPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLD
YSRAEVNEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGLLTQSYQFWREGTEIDL
GADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPALPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSL
RLNGQLITVAEESVFCLGDFILPFNGTATKHQGDE

Sequences:

>Translated_755_residues
MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQTRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAING
EVFSLSHEAWQRELDFASGELRRNVVWRTSNGSGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
RQHLDETQVRVFGQHLMQGSYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTSAQLHAGETMTLQKLVWIDWRDD
RQAALDEWGSASLRQLEMCAQQSYDQLLAASTENWRQWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAK
GLTGEGYKGHVFWDTEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETPEFAAINIR
TGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAKFWISRAVRVNDRLEIHDVIGPDEYTEHVNN
NAYTSYMARYNVQQALNIARQFGCSDDAFIHRAEMFLKELWMPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLD
YSRAEVNEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGLLTQSYQFWREGTEIDL
GADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPALPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSL
RLNGQLITVAEESVFCLGDFILPFNGTATKHQGDE
>Mature_754_residues
TRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQTRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGE
VFSLSHEAWQRELDFASGELRRNVVWRTSNGSGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHGR
QHLDETQVRVFGQHLMQGSYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTSAQLHAGETMTLQKLVWIDWRDDR
QAALDEWGSASLRQLEMCAQQSYDQLLAASTENWRQWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKG
LTGEGYKGHVFWDTEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETPEFAAINIRT
GLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAKFWISRAVRVNDRLEIHDVIGPDEYTEHVNNN
AYTSYMARYNVQQALNIARQFGCSDDAFIHRAEMFLKELWMPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDY
SRAEVNEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGLLTQSYQFWREGTEIDLG
ADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPALPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLR
LNGQLITVAEESVFCLGDFILPFNGTATKHQGDE

Specific function: Unknown

COG id: COG1554

COG function: function code G; Trehalose and maltose hydrolases (possible phosphorylases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 65 family [H]

Homologues:

Organism=Homo sapiens, GI187829418, Length=354, Percent_Identity=28.5310734463277, Blast_Score=109, Evalue=8e-24,
Organism=Escherichia coli, GI1787575, Length=755, Percent_Identity=99.8675496688742, Blast_Score=1569, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6325283, Length=396, Percent_Identity=22.979797979798, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24583760, Length=393, Percent_Identity=26.972010178117, Blast_Score=110, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR012341
- InterPro:   IPR011013
- InterPro:   IPR005194
- InterPro:   IPR005195
- InterPro:   IPR005196
- InterPro:   IPR017045 [H]

Pfam domain/function: PF03633 Glyco_hydro_65C; PF03632 Glyco_hydro_65m; PF03636 Glyco_hydro_65N [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 84796; Mature: 84665

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQTRGMYLAGLYHRAGKG
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHCCEEEEEHHHCCCCC
EINELVNLPDVVGMEIAINGEVFSLSHEAWQRELDFASGELRRNVVWRTSNGSGYTIASR
CHHHHCCCCCCCCEEEEECCEEEEECHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEECC
RFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHGRQHLDETQVRVFGQHLMQGS
EEECCCCCCEEEEEEEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
YTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTSAQLHAGETMTLQKLVWIDWRDD
CCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCC
RQAALDEWGSASLRQLEMCAQQSYDQLLAASTENWRQWWQKRRITVNGGEAHDQQALDYA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEECCCCCCHHHHHHHH
LYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWDTEVFLLPFHLFSDPTVARSLLRYRWH
HHEEEEECCCCCCCHHHHCCCCCCCCEEEEEEECCEEEEEEEHHCCCCHHHHHHHHHHHC
NLPGAQEKARRNGWQGALFPWESARSGEEETPEFAAINIRTGLRQKVASAQAEHHLVADI
CCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHH
AWAVIQYWQTTGDESFIAHEGMALLLETAKFWISRAVRVNDRLEIHDVIGPDEYTEHVNN
HHHHHHHHHCCCCCCEEHHCCHHHHHHHHHHHHHHHHEECCCEEEEECCCCHHHHHHCCC
NAYTSYMARYNVQQALNIARQFGCSDDAFIHRAEMFLKELWMPEIQPDGVLPQDDSFMAK
CHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
PAINLAKYKAAAGKQTILLDYSRAEVNEMQILKQADVVMLNYMLPEQFSAASCLANLQFY
CCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHCCEEC
EPRTIHDSSLSKAIHGIVAARCGLLTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIW
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHHHHHHHH
LGAIQGFAGVSVRDGELHLNPALPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSL
HHHHHCCCCCEEECCEEEECCCCCHHHHHCCCCEEECCCEEEEEECCEEEEEEECCCEEE
RLNGQLITVAEESVFCLGDFILPFNGTATKHQGDE
EECCEEEEEECCCEEEEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
TRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQTRGMYLAGLYHRAGKG
CCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHCCEEEEEHHHCCCCC
EINELVNLPDVVGMEIAINGEVFSLSHEAWQRELDFASGELRRNVVWRTSNGSGYTIASR
CHHHHCCCCCCCCEEEEECCEEEEECHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEECC
RFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHGRQHLDETQVRVFGQHLMQGS
EEECCCCCCEEEEEEEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
YTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTSAQLHAGETMTLQKLVWIDWRDD
CCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCC
RQAALDEWGSASLRQLEMCAQQSYDQLLAASTENWRQWWQKRRITVNGGEAHDQQALDYA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEECCCCCCHHHHHHHH
LYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWDTEVFLLPFHLFSDPTVARSLLRYRWH
HHEEEEECCCCCCCHHHHCCCCCCCCEEEEEEECCEEEEEEEHHCCCCHHHHHHHHHHHC
NLPGAQEKARRNGWQGALFPWESARSGEEETPEFAAINIRTGLRQKVASAQAEHHLVADI
CCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHH
AWAVIQYWQTTGDESFIAHEGMALLLETAKFWISRAVRVNDRLEIHDVIGPDEYTEHVNN
HHHHHHHHHCCCCCCEEHHCCHHHHHHHHHHHHHHHHEECCCEEEEECCCCHHHHHHCCC
NAYTSYMARYNVQQALNIARQFGCSDDAFIHRAEMFLKELWMPEIQPDGVLPQDDSFMAK
CHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
PAINLAKYKAAAGKQTILLDYSRAEVNEMQILKQADVVMLNYMLPEQFSAASCLANLQFY
CCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHCCEEC
EPRTIHDSSLSKAIHGIVAARCGLLTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIW
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHHHHHHHH
LGAIQGFAGVSVRDGELHLNPALPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSL
HHHHHCCCCCEEECCEEEECCCCCHHHHHCCCCEEECCCEEEEEECCEEEEEEECCCEEE
RLNGQLITVAEESVFCLGDFILPFNGTATKHQGDE
EECCEEEEEECCCEEEEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]