Click here to switch to the map view.

The map label for this gene is pspA

Identifier: 157160815

GI number: 157160815

Start: 1415530

End: 1416198

Strand: Direct

Name: pspA

Synonym: EcHS_A1419

Alternate gene names: 157160815

Gene position: 1415530-1416198 (Clockwise)

Preceding gene: 157160813

Following gene: 157160816

Centisome position: 30.48

GC content: 49.03

Gene sequence:

>669_bases
ATGGGTATTTTTTCTCGCTTTGCCGACATCGTGAATGCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAA
ACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACATTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCAGAAA
AGAAACAGCTGACTCGCCGTATTGAACAAGCGTCGGCGCGTGAAGTTGAATGGCAGGAAAAAGCCGAACTGGCGCTGCTG
AAAGAGAGAGAGGATCTGGCGCGTGCGGCGTTAATTGAAAAACAGAAACTGACCGATCTGATTAAGAGCCTGGAACATGA
AGTGACGCTGGTGGACGATACGCTGGCACGCATGAAGAAAGAGATTGGCGAGCTGGAAAACAAATTGAGCGAAACACGCG
CTCGCCAGCAGGCATTGATGTTACGCCATCAGGCGGCAAATTCGTCGCGCGATGTGCGTCGTCAGTTGGACAGTGGCAAA
CTGGATGAAGCAATGGCTCGTTTCGAATCTTTCGAACGTCGTATTGACCAGATGGAAGCAGAAGCGGAAAGCCACAGCTT
CGGTAAACAAAAATCGCTGGACGATCAATTTGCCGAACTGAAAGCCGATGATGCAATAAGCGAGCAACTGGCACAATTAA
AAGCCAAAATGAAGCAAGACAATCAATAA

Upstream 100 bases:

>100_bases
ATCAGATCTTTATAAATCAAAAAGATAAAAAATTGGCACGCAAATTGTATTAACTGTTCAGCAGGACAATCCTGAACGCA
GAAATCAAGAGGACAACATT

Downstream 100 bases:

>100_bases
TAACATCCAGGCGGCGTCCGAAAGCGCCGCCGCTCATCGTCTAAGGAGTACTTATGAGCGCGCTATTTCTGGCTATTCCA
TTAACCATTTTTGTGCTGTT

Product: phage shock protein PspA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVRSTSARALAEKKQLTRRIEQASAREVEWQEKAELALL
KEREDLARAALIEKQKLTDLIKSLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMKQDNQ

Sequences:

>Translated_222_residues
MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVRSTSARALAEKKQLTRRIEQASAREVEWQEKAELALL
KEREDLARAALIEKQKLTDLIKSLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMKQDNQ
>Mature_221_residues
GIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVRSTSARALAEKKQLTRRIEQASAREVEWQEKAELALLK
EREDLARAALIEKQKLTDLIKSLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGKL
DEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMKQDNQ

Specific function: The phage shock protein (psp) operon (pspABCDE) may play a significant role in the competition for survival under nutrient- or energy-limited conditions. PspA negatively regulates expression of the pspABCDE promoter and of pspG through negative regulation

COG id: COG1842

COG function: function code KT; Phage shock protein A (IM30), suppresses sigma54-dependent transcription

Gene ontology:

Cell location: Cytoplasm. Cell inner membrane; Peripheral membrane protein; Cytoplasmic side (By similarity)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PspA/IM30 family

Homologues:

Organism=Escherichia coli, GI2367118, Length=222, Percent_Identity=100, Blast_Score=433, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PSPA_ECO57 (P0AFM7)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A99864
- PIR:   H85754
- RefSeq:   NP_287884.1
- RefSeq:   NP_309908.1
- ProteinModelPortal:   P0AFM7
- EnsemblBacteria:   EBESCT00000026316
- EnsemblBacteria:   EBESCT00000057771
- GeneID:   912684
- GeneID:   961336
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z2482
- KEGG:   ecs:ECs1881
- GeneTree:   EBGT00050000011178
- HOGENOM:   HBG512970
- OMA:   KILDQSV
- ProtClustDB:   PRK10698
- BioCyc:   ECOL83334:ECS1881-MONOMER
- GO:   GO:0005737
- InterPro:   IPR014319
- InterPro:   IPR007157
- TIGRFAMs:   TIGR02977

Pfam domain/function: PF04012 PspA_IM30

EC number: NA

Molecular weight: Translated: 25493; Mature: 25362

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVRSTSARALAEKKQLTRR
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIKSLEHEVTLVDDTLARMKK
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGKLDEAMARFESFERRIDQMEA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
EAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMKQDNQ
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVRSTSARALAEKKQLTRR
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIKSLEHEVTLVDDTLARMKK
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGKLDEAMARFESFERRIDQMEA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
EAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMKQDNQ
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796