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The map label for this gene is trpD

Identifier: 157160768

GI number: 157160768

Start: 1366846

End: 1368441

Strand: Reverse

Name: trpD

Synonym: EcHS_A1372

Alternate gene names: 157160768

Gene position: 1368441-1366846 (Counterclockwise)

Preceding gene: 157160769

Following gene: 157160767

Centisome position: 29.47

GC content: 56.39

Gene sequence:

>1596_bases
ATGGCTGACATTCTGCTGCTCGATAATATCGACTCTTTTACGTACAACCTGGCAGATCAGTTGCGCAGCAATGGGCATAA
CGTGGTGATTTACCGCAACCATATTCCGGCGCAAACCTTAATTGAACGCCTGGCGACCATGAGCAATCCGGTGCTGATGC
TTTCTCCTGGCCCCGGTGTGCCGAGCGAAGCCGGTTGTATGCCGGAACTCCTCACCCGCTTGCGTGGCAAGCTGCCCATT
ATTGGCATTTGCCTCGGACATCAGGCGATTGTCGAAGCTTACGGGGGCTATGTCGGTCAGGCGGGCGAAATTCTCCACGG
TAAAGCCTCCAGCATTGAACATGACGGTCAGGCGATGTTTGCCGGATTAACAAACCCGCTGCCGGTGGCGCGTTATCACT
CGCTGGTTGGCAGTAACATTCCGGCCGGTTTAACCATCAACGCCCATTTTAATGGCATGGTGATGGCAGTACGTCACGAT
GCGGATCGCGTTTGTGGATTCCAGTTCCATCCGGAATCCATTCTCACCACCCAGGGCGCTCGCCTGCTGGAACAAACGCT
GGCCTGGGCGCAGCAGAAACTAGAGCCAGCCAACACGCTGCAACCGATTCTGGAAAAACTGTATCAGGCGCAGACGCTTA
GCCAACAAGAAAGCCACCAGCTGTTTTCAGCGGTGGTGCGTGGCGAGCTGAAGCCGGAACAACTGGCGGCGGCGCTGGTG
AGCATGAAAATTCGCGGTGAGCACCCGAACGAGATCGCCGGGGCAGCAACCGCGCTACTGGAAAACGCAGCGCCGTTCCC
GCGCCCGGATTATCTGTTTGCTGATATCGTCGGTACTGGCGGTGACGGCAGCAACAGTATCAATATTTCTACCGCCAGTG
CGTTTGTCGCCGCGGCCTGTGGGCTGAAAGTGGCGAAACACGGCAACCGTAGCGTCTCCAGTAAATCTGGTTCGTCCGAT
CTGCTGGCGGCGTTCGGTATTAATCTTGATATGAACGCCGATAAATCGCGCCAGGCGCTGGATGAGTTAGGTGTATGTTT
CCTCTTTGCGCCGAAGTATCACACCGGATTCCGCCACGCGATGCCGGTTCGCCAGCAACTGAAAACCCGCACCCTGTTCA
ATGTGCTGGGGCCATTGATTAACCCGGCGCATCCGCCGCTGGCGTTAATTGGTGTTTATAGTCCGGAACTGGTGCTGCCG
ATTGCCGAAACCTTGCGCGTGCTGGGGTATCAACGCGCGGCGGTGGTGCACAGCGGCGGGATGGATGAAGTTTCATTACA
CGCGCCGACAATCGTTGCCGAACTGCATGACGGCGAAATTAAAAGCTATCAGCTCACCGCAGAAGACTTTGGCCTGACAC
CCTACCACCAGGAGCAACTGGCAGGCGGAACACCGGAAGAAAACCGTGACATTTTAACACGTTTGTTACAAGGTAAAGGC
GACGCCGCCCATGAAGCAGCCGTCGCTGCGAACGTCGCCATGTTAATGCGCCTGCATGGCCATGAAGATCTGCAAGCCAA
TGCGCAAACCGTTCTTGAGGTACTGCGCAGTGGTTCCGCTTACGACAGAGTCACCGCACTGGCGGCACGAGGGTAA

Upstream 100 bases:

>100_bases
TTGATTCTGTTCCGCAGTCGGAAGCCGACGAAACCCGTAATAAAGCCCGCGCTGTACTGCGCGCTATTGCCACCGCGCAT
CATGCACAGGAGACTTTCTG

Downstream 100 bases:

>100_bases
ATGATGCAAACCGTTTTAGCGAAAATCGTCGCAGACAAGGCGATTTGGGTAGAAGCCCGCAAACAGCAGCAACCGCTGGC
CAGTTTTCAGAATGAGGTTC

Product: bifunctional glutamine amidotransferase/anthranilate phosphoribosyltransferase

Products: NA

Alternate protein names: Glutamine amidotransferase; Anthranilate phosphoribosyltransferase

Number of amino acids: Translated: 531; Mature: 530

Protein sequence:

>531_residues
MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERLATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPI
IGICLGHQAIVEAYGGYVGQAGEILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQESHQLFSAVVRGELKPEQLAAALV
SMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADIVGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSD
LLAAFGINLDMNADKSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGVYSPELVLP
IAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDFGLTPYHQEQLAGGTPEENRDILTRLLQGKG
DAAHEAAVAANVAMLMRLHGHEDLQANAQTVLEVLRSGSAYDRVTALAARG

Sequences:

>Translated_531_residues
MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERLATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPI
IGICLGHQAIVEAYGGYVGQAGEILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQESHQLFSAVVRGELKPEQLAAALV
SMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADIVGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSD
LLAAFGINLDMNADKSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGVYSPELVLP
IAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDFGLTPYHQEQLAGGTPEENRDILTRLLQGKG
DAAHEAAVAANVAMLMRLHGHEDLQANAQTVLEVLRSGSAYDRVTALAARG
>Mature_530_residues
ADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERLATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPII
GICLGHQAIVEAYGGYVGQAGEILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHDA
DRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQESHQLFSAVVRGELKPEQLAAALVS
MKIRGEHPNEIAGAATALLENAAPFPRPDYLFADIVGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDL
LAAFGINLDMNADKSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGVYSPELVLPI
AETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDFGLTPYHQEQLAGGTPEENRDILTRLLQGKGD
AAHEAAVAANVAMLMRLHGHEDLQANAQTVLEVLRSGSAYDRVTALAARG

Specific function: Tryptophan biosynthesis; first step. Tryptophan biosynthesis; second step. [C]

COG id: COG0547

COG function: function code E; Anthranilate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1787517, Length=531, Percent_Identity=100, Blast_Score=1083, Evalue=0.0,
Organism=Escherichia coli, GI1789760, Length=187, Percent_Identity=43.8502673796791, Blast_Score=151, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6322638, Length=227, Percent_Identity=37.0044052863436, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6320561, Length=323, Percent_Identity=33.1269349845201, Blast_Score=130, Evalue=6e-31,

Paralogues:

None

Copy number: 945 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPG_ECOLI (P00904)

Other databases:

- EMBL:   V00367
- EMBL:   V00372
- EMBL:   J01714
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   B64874
- RefSeq:   AP_001889.1
- RefSeq:   NP_415779.1
- ProteinModelPortal:   P00904
- STRING:   P00904
- SWISS-2DPAGE:   P00904
- EnsemblBacteria:   EBESCT00000002039
- EnsemblBacteria:   EBESCT00000016289
- GeneID:   945109
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1255
- KEGG:   eco:b1263
- EchoBASE:   EB1020
- EcoGene:   EG11027
- eggNOG:   COG0547
- GeneTree:   EBGT00050000009095
- HOGENOM:   HBG559996
- OMA:   SPGPKHP
- ProtClustDB:   PRK09522
- BioCyc:   EcoCyc:ANTHRANSYNCOMPII-MONOMER
- BioCyc:   MetaCyc:ANTHRANSYNCOMPII-MONOMER
- Genevestigator:   P00904
- HAMAP:   MF_00211
- InterPro:   IPR005940
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR000312
- InterPro:   IPR017459
- InterPro:   IPR020072
- InterPro:   IPR006221
- Gene3D:   G3DSA:1.20.970.10
- Gene3D:   G3DSA:3.40.1030.10
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR01245
- TIGRFAMs:   TIGR00566

Pfam domain/function: PF00117 GATase; PF02885 Glycos_trans_3N; PF00591 Glycos_transf_3; SSF47648 Glyco_trans_3; SSF52418 Glyco_trans_3

EC number: =4.1.3.27; =2.4.2.18

Molecular weight: Translated: 56871; Mature: 56740

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 84-84 ACT_SITE 170-170 ACT_SITE 172-172

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERLATMSNPVLMLSPGPGV
CCCEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCC
PSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAGEILHGKASSIEHDGQAMF
CCCCCCHHHHHHHHCCCCCEEEEECCHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCEEE
AGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHDADRVCGFQFHPESILTTQGA
ECCCCCCCHHHHHHHHCCCCCCCEEEEECCCCEEEEEECCCCHHCCCEECCHHHHHHHHH
RLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQESHQLFSAVVRGELKPEQLAAALV
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
SMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADIVGTGGDGSNSINISTASAFVAAAC
HHHCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCEEEEEHHHHHHHHHH
GLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNADKSRQALDELGVCFLFAPKYHTGFRHA
HHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHCCEEEEECCCCCCCCHHH
MPVRQQLKTRTLFNVLGPLINPAHPPLALIGVYSPELVLPIAETLRVLGYQRAAVVHSGG
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCHHHEEEECCC
MDEVSLHAPTIVAELHDGEIKSYQLTAEDFGLTPYHQEQLAGGTPEENRDILTRLLQGKG
CCCEEECCCEEEEEECCCCCCEEEEEHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHCCC
DAAHEAAVAANVAMLMRLHGHEDLQANAQTVLEVLRSGSAYDRVTALAARG
CHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure 
ADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERLATMSNPVLMLSPGPGV
CCEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCC
PSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAGEILHGKASSIEHDGQAMF
CCCCCCHHHHHHHHCCCCCEEEEECCHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCEEE
AGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHDADRVCGFQFHPESILTTQGA
ECCCCCCCHHHHHHHHCCCCCCCEEEEECCCCEEEEEECCCCHHCCCEECCHHHHHHHHH
RLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQESHQLFSAVVRGELKPEQLAAALV
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
SMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADIVGTGGDGSNSINISTASAFVAAAC
HHHCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCEEEEEHHHHHHHHHH
GLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNADKSRQALDELGVCFLFAPKYHTGFRHA
HHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHCCEEEEECCCCCCCCHHH
MPVRQQLKTRTLFNVLGPLINPAHPPLALIGVYSPELVLPIAETLRVLGYQRAAVVHSGG
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCHHHEEEECCC
MDEVSLHAPTIVAELHDGEIKSYQLTAEDFGLTPYHQEQLAGGTPEENRDILTRLLQGKG
CCCEEECCCEEEEEECCCCCCEEEEEHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHCCC
DAAHEAAVAANVAMLMRLHGHEDLQANAQTVLEVLRSGSAYDRVTALAARG
CHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7038627; 6283099; 9097039; 9278503; 4594441