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The map label for this gene is trpE [H]

Identifier: 157160769

GI number: 157160769

Start: 1368441

End: 1370003

Strand: Reverse

Name: trpE [H]

Synonym: EcHS_A1373

Alternate gene names: 157160769

Gene position: 1370003-1368441 (Counterclockwise)

Preceding gene: 157160770

Following gene: 157160768

Centisome position: 29.5

GC content: 54.38

Gene sequence:

>1563_bases
ATGCAAACACAAAAACCGACTCTCGAACTGCTAACCTGCGAAGGCGCTTATCGCGACAATCCCACCGCGCTTTTTCACCA
ATTGTGTGAGGATCGTCCGGCAACGCTGCTGCTGGAATCCGCAGATATCGACAGCAAAGATGATTTAAAAAGCCTGCTGC
TAGTAGACAGTGCGCTACGCATTACAGCTTTAGGTGACACTGTCACAATCCAGTCCCTTTCCGGCAACGGCGAAGCCCTG
CTGACACTACTGGATAACGCCCTGCCTGCGGGTGTGGAAAATGAACAATTACCAAACTGCCGTGTGCTGCGCTTCCCCCC
TGTTAGTCCACTGCTGGATGAAGACGCTCGCTTATGCTCCCTTTCGGTTTTTGACGCTTTCCGCTTATTGCAGAATCTGT
TGAATGTACCGAAGGAAGAACGAGAAGCCATGTTCTTCGGCGGCCTGTTCTCTTATGACCTTGTGGCGGGGTTTGAAGAT
TTACCGCAACTGTCAGCGGAAAATAACTGCCCTGATTTCTGTTTTTATCTCGCTGAAACGCTGATGGTGATTGACCATCA
GAAAAAAAGCACCCGCATTCAGGCCAGCCTGTTTGCTCCGAATGAAGAAGAAAAACAACGTCTCACTGCTCGCCTGAACG
ATCTTCGCCAGCAACTGACCGAAACCGCGCCACCGCTGCCGGTGGTTTCCGTGCCGCATATGCGTTGTGAATGTAACCAG
AGCGATGAAGAGTTCGGTGGCGTAGTGCGTTTGTTGCAAAAAGCGATTCGCGCTGGAGAAATTTTCCAGGTGGTGCCATC
TCGCCGTTTCTCTCTGCCCTGCCCGTCACCGCTGGCGGCCTATTACGTGCTGAAAAAGAGTAATCCCAGCCCGTACATGT
TTTTTATGCAGGATAATGATTTCACCCTGTTTGGCGCGTCGCCGGAAAGTTCGCTCAAATATGACGCCACCAGCCGCCAG
ATTGAGATCTACCCGATTGCCGGGACACGCCCACGCGGTCGTCGTGCCGATGGTTCACTGGACAGAGACCTCGACAGCCG
CATCGAACTGGAAATGCGTACCGATCATAAAGAGCTTTCTGAACATCTGATGCTGGTGGATCTCGCCCGTAATGATCTGG
CACGCATTTGCACCCCCGGCAGCCGCTACGTCGCCGATCTTACCAAAGTTGACCGTTACTCTTACGTGATGCACCTGGTC
TCCCGCGTGGTCGGTGAGCTGCGCCACGATCTCGACGCCCTGCACGCTTACCGCGCCTGTATGAATATGGGAACGTTAAG
CGGTGCGCCGAAAGTACGCGCTATGCAGTTAATTGCCGAGGCTGAAGGTCGTCGCCGCGGCAGCTACGGCGGCGCGGTAG
GTTATTTCACCGCGCATGGCGATCTCGACACCTGCATTGTGATCCGCTCGGCGCTGGTGGAAAACAGTATCGCCACCGTG
CAAGCCGGTGCTGGCGTAGTCCTTGATTCTGTTCCGCAGTCGGAAGCCGACGAAACCCGTAATAAAGCCCGCGCTGTACT
GCGCGCTATTGCCACCGCGCATCATGCACAGGAGACTTTCTGA

Upstream 100 bases:

>100_bases
ACTTCCTGAAACGGGCAGTGTATTCACCATGCGTAAAGCAATCAGATACCCAGCCCGCCTAATGAGCGGGCTTTTTTTTG
AACAAAATTAGAGAATAACA

Downstream 100 bases:

>100_bases
TGGCTGACATTCTGCTGCTCGATAATATCGACTCTTTTACGTACAACCTGGCAGATCAGTTGCGCAGCAATGGGCATAAC
GTGGTGATTTACCGCAACCA

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MQTQKPTLELLTCEGAYRDNPTALFHQLCEDRPATLLLESADIDSKDDLKSLLLVDSALRITALGDTVTIQSLSGNGEAL
LTLLDNALPAGVENEQLPNCRVLRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQLTETAPPLPVVSVPHMRCECNQ
SDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPSPLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQ
IEIYPIAGTRPRGRRADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDRYSYVMHLV
SRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYGGAVGYFTAHGDLDTCIVIRSALVENSIATV
QAGAGVVLDSVPQSEADETRNKARAVLRAIATAHHAQETF

Sequences:

>Translated_520_residues
MQTQKPTLELLTCEGAYRDNPTALFHQLCEDRPATLLLESADIDSKDDLKSLLLVDSALRITALGDTVTIQSLSGNGEAL
LTLLDNALPAGVENEQLPNCRVLRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQLTETAPPLPVVSVPHMRCECNQ
SDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPSPLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQ
IEIYPIAGTRPRGRRADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDRYSYVMHLV
SRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYGGAVGYFTAHGDLDTCIVIRSALVENSIATV
QAGAGVVLDSVPQSEADETRNKARAVLRAIATAHHAQETF
>Mature_520_residues
MQTQKPTLELLTCEGAYRDNPTALFHQLCEDRPATLLLESADIDSKDDLKSLLLVDSALRITALGDTVTIQSLSGNGEAL
LTLLDNALPAGVENEQLPNCRVLRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQLTETAPPLPVVSVPHMRCECNQ
SDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPSPLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQ
IEIYPIAGTRPRGRRADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDRYSYVMHLV
SRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYGGAVGYFTAHGDLDTCIVIRSALVENSIATV
QAGAGVVLDSVPQSEADETRNKARAVLRAIATAHHAQETF

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=520, Percent_Identity=98.4615384615385, Blast_Score=1048, Evalue=0.0,
Organism=Escherichia coli, GI1788114, Length=422, Percent_Identity=29.3838862559242, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI87082077, Length=299, Percent_Identity=27.7591973244147, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1786809, Length=227, Percent_Identity=29.0748898678414, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320935, Length=399, Percent_Identity=36.8421052631579, Blast_Score=228, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6324361, Length=274, Percent_Identity=27.7372262773723, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005257
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 57712; Mature: 57712

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTQKPTLELLTCEGAYRDNPTALFHQLCEDRPATLLLESADIDSKDDLKSLLLVDSALR
CCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCEE
ITALGDTVTIQSLSGNGEALLTLLDNALPAGVENEQLPNCRVLRFPPVSPLLDEDARLCS
EEECCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHH
LSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFEDLPQLSAENNCPDFCFYLAET
HHHHHHHHHHHHHHCCCHHHHCEEEECCHHHHHHHHCHHHHHHCCCCCCCHHHHHHHHHH
LMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQLTETAPPLPVVSVPHMRCECNQ
HHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCEECCCC
SDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPSPLAAYYVLKKSNPSPYMFFMQDND
CHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHEEEEEECCCCCCEEEEEECCC
FTLFGASPESSLKYDATSRQIEIYPIAGTRPRGRRADGSLDRDLDSRIELEMRTDHKELS
EEEEECCCCCCCEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHH
EHLMLVDLARNDLARICTPGSRYVADLTKVDRYSYVMHLVSRVVGELRHDLDALHAYRAC
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNMGTLSGAPKVRAMQLIAEAEGRRRGSYGGAVGYFTAHGDLDTCIVIRSALVENSIATV
HHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCHHEE
QAGAGVVLDSVPQSEADETRNKARAVLRAIATAHHAQETF
ECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQTQKPTLELLTCEGAYRDNPTALFHQLCEDRPATLLLESADIDSKDDLKSLLLVDSALR
CCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCEE
ITALGDTVTIQSLSGNGEALLTLLDNALPAGVENEQLPNCRVLRFPPVSPLLDEDARLCS
EEECCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHH
LSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFEDLPQLSAENNCPDFCFYLAET
HHHHHHHHHHHHHHCCCHHHHCEEEECCHHHHHHHHCHHHHHHCCCCCCCHHHHHHHHHH
LMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQLTETAPPLPVVSVPHMRCECNQ
HHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCEECCCC
SDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPSPLAAYYVLKKSNPSPYMFFMQDND
CHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHEEEEEECCCCCCEEEEEECCC
FTLFGASPESSLKYDATSRQIEIYPIAGTRPRGRRADGSLDRDLDSRIELEMRTDHKELS
EEEEECCCCCCCEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHH
EHLMLVDLARNDLARICTPGSRYVADLTKVDRYSYVMHLVSRVVGELRHDLDALHAYRAC
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNMGTLSGAPKVRAMQLIAEAEGRRRGSYGGAVGYFTAHGDLDTCIVIRSALVENSIATV
HHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCHHEE
QAGAGVVLDSVPQSEADETRNKARAVLRAIATAHHAQETF
ECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7038627; 7021857; 9097039; 9278503; 351195; 4598537 [H]