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The map label for this gene is dsbB

Identifier: 157160688

GI number: 157160688

Start: 1280939

End: 1281469

Strand: Reverse

Name: dsbB

Synonym: EcHS_A1288

Alternate gene names: 157160688

Gene position: 1281469-1280939 (Counterclockwise)

Preceding gene: 157160689

Following gene: 157160685

Centisome position: 27.6

GC content: 52.73

Gene sequence:

>531_bases
ATGTTGCGATTTTTGAACCAATGTTCACAAGGCCGGGGCGCGTGGCTGTTGATGGCGTTTACTGCTCTGGCACTGGAACT
GACGGCGCTGTGGTTCCAGCATGTGATGTTACTGAAACCTTGCGTGCTCTGTATTTATGAACGCTGCGCGTTATTCGGCG
TTCTGGGTGCTGCGCTGATTGGCGCGATCGCCCCGAAAACTCCGCTGCGTTATGTAGCGATGGTTATCTGGTTGTATAGT
GCGTTCCGCGGTGTGCAGTTAACTTACGAGCACACCATGCTTCAGCTCTATCCTTCGCCGTTTGCCACCTGTGATTTTAT
GGTTCGTTTCCCGGAATGGCTGCCGCTGGATAAGTGGGTGCCGCAAGTGTTTGTCGCCTCTGGCGATTGCGCCGAGCGTC
AGTGGGATTTTTTAGGTCTGGAAATGCCGCAGTGGCTGCTCGGTATTTTTATCGCTTACCTGATTGTCGCAGTGCTGGTG
GTGATTTCCCAGCCGTTTAAAGCGAAAAAACGTGATCTGTTCGGTCGCTAA

Upstream 100 bases:

>100_bases
GATAATTGTCCAATTGCGTCCTATTTTTGCTGCCTCCTGGTGGCGCAGCGAATGAATTGGTTTAAACTGCGCACTCTATG
CATATTGCAGGGAAATGATT

Downstream 100 bases:

>100_bases
TCCATTCGGCGCTCCTGCGGGAGCGCTTTTTTCCTGCCGCTATATTTATTTGACCCTCAGTAAATCAGAACTTCGCGTTG
TATAACGTGGTGAAAGCATG

Product: disulfide bond formation protein B

Products: NA

Alternate protein names: Disulfide oxidoreductase

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLCIYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYS
AFRGVQLTYEHTMLQLYPSPFATCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
VISQPFKAKKRDLFGR

Sequences:

>Translated_176_residues
MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLCIYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYS
AFRGVQLTYEHTMLQLYPSPFATCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
VISQPFKAKKRDLFGR
>Mature_176_residues
MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLCIYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYS
AFRGVQLTYEHTMLQLYPSPFATCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
VISQPFKAKKRDLFGR

Specific function: Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by oxidizing the DsbA protein

COG id: COG1495

COG function: function code O; Disulfide bond formation protein DsbB

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DsbB family

Homologues:

Organism=Escherichia coli, GI1787433, Length=176, Percent_Identity=100, Blast_Score=357, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DSBB_ECO57 (P0A6M3)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   H90838
- RefSeq:   NP_287424.1
- RefSeq:   NP_309707.1
- ProteinModelPortal:   P0A6M3
- SMR:   P0A6M3
- EnsemblBacteria:   EBESCT00000028567
- EnsemblBacteria:   EBESCT00000059109
- GeneID:   913192
- GeneID:   959898
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z1948
- KEGG:   ecs:ECs1680
- GeneTree:   EBGT00050000011288
- HOGENOM:   HBG637909
- OMA:   ALYFQHV
- ProtClustDB:   PRK01749
- BioCyc:   ECOL83334:ECS1680-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_00286
- InterPro:   IPR003752
- InterPro:   IPR022920

Pfam domain/function: PF02600 DsbB

EC number: NA

Molecular weight: Translated: 20142; Mature: 20142

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x13c2d468)-; HASH(0x13cf8d18)-; HASH(0x13851378)-; HASH(0x13ca8200)-;

Cys/Met content:

3.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLCIYERCALFGVLGAALI
CCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFATCDFMVRFPEWLPLDKWV
HHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHH
PQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLVVISQPFKAKKRDLFGR
HHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCH
>Mature Secondary Structure
MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLCIYERCALFGVLGAALI
CCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFATCDFMVRFPEWLPLDKWV
HHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHH
PQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLVVISQPFKAKKRDLFGR
HHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796