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The map label for this gene is nhaB [H]

Identifier: 157160689

GI number: 157160689

Start: 1281615

End: 1283156

Strand: Reverse

Name: nhaB [H]

Synonym: EcHS_A1289

Alternate gene names: 157160689

Gene position: 1283156-1281615 (Counterclockwise)

Preceding gene: 157160691

Following gene: 157160688

Centisome position: 27.63

GC content: 52.85

Gene sequence:

>1542_bases
ATGGAGATCTCCTGGGGCCGCGCGCTATGGCGCAATTTTTTGGGCCAGTCCCCCGACTGGTACAAACTCGCCCTCATCAT
TTTCTTAATCGTAAACCCGTTAATTTTCCTCATCAGCCCTTTCGTCGCGGGCTGGTTGCTGGTAGCGGAATTTATTTTCA
CTCTGGCGATGGCCCTGAAATGCTACCCGCTGCTCCCCGGTGGTCTGTTGGCTATCGAAGCGGTATTCATCGGCATGACC
AGCGCGGAACACGTCCGTGAAGAGGTGGCGGCAAATCTTGAAGTCTTGCTGTTACTGATGTTTATGGTGGCGGGTATCTA
TTTTATGAAACAGCTGTTGCTGTTCATATTTACCCGTTTGCTGTTAAGCATTCGCTCCAAAATGCTGCTGTCGCTCTCTT
TTTGCGTGGCGGCTGCGTTCCTCTCCGCGTTCCTCGATGCCTTAACCGTCGTGGCGGTGGTGATCAGCGTTGCAGTCGGT
TTTTATGGTATTTATCACCGCGTAGCCTCTTCCCGTACCGAAGACACCGACCTGCAAGACGATAGTCATATCGACAAGCA
TTACAAAGTGGTTCTGGAACAGTTCCGTGGCTTTCTGCGTAGCCTGATGATGCATGCCGGTGTCGGCACCGCATTAGGCG
GCGTAATGACCATGGTGGGCGAACCACAGAACCTGATCATCGCTAAAGCGGCTGGCTGGCATTTTGGCGATTTCTTCCTG
CGCATGTCGCCGGTGACCGTTCCGGTTCTGATTTGTGGCCTGTTAACCTGCCTGCTGGTAGAGAAGCTGCGTTGGTTTGG
CTACGGTGAAACGCTGCCGGAGAAAGTCCGCGAAGTGTTGCAACAGTTTGACGATCAAAGCCGCCACCAGCGTACCCGTC
AGGATAAAATCCGTCTGATTGTCCAGGCGATTATTGGCGTCTGGCTGGTGACTGCGCTGGCGTTGCATCTGGCGGAAGTT
GGCTTGATTGGTTTGTCAGTCATTATTCTGGCAACATCATTGACCGGTGTCACCGATGAGCATGCTATCGGTAAAGCCTT
CACCGAATCTCTGCCATTCACCGCACTGTTGACGGTGTTTTTCTCGGTAGTCGCGGTGATTATCGACCAACAACTGTTTT
CGCCAATTATTCAGTTTGTGTTGCAGGCATCGGAACATGCTCAGCTGTCGCTGTTCTATATTTTCAACGGTCTGCTGTCA
TCCATTTCGGATAACGTCTTCGTGGGGACGATTTATATCAACGAAGCGAAAGCGGCAATGGAAAGTGGCGCTATCACGTT
GAAGCAATACGAGCTGCTGGCGGTCGCCATTAATACCGGTACCAATCTGCCCTCCGTCGCTACGCCGAACGGTCAGGCTG
CGTTCCTGTTCCTGCTGACCTCTGCACTCGCGCCATTGATTCGCCTCTCTTATGGCCGCATGGTGTGGATGGCCCTGCCT
TACACCCTCGTCCTGACACTCGTCGGCTTGCTCTGCGTCGAGTTTACGCTTGCCCCTGTAACCGAATGGTTTATGCAAAT
GGGCTGGATAGCAACGCTTTGA

Upstream 100 bases:

>100_bases
CCCTTGACTATCAAGACTTGCCTCACTCCTGCCTGACCGTTAAGCTTTGCGTTTAAACATAAAAACATCATTGATTATTT
GTAAGGTAAGGGAAACCATC

Downstream 100 bases:

>100_bases
TAACAACTTACCGGGCATATAAATGCCCGGTTTGCCTTTTCGCCCGATAATTGTCCAATTGCGTCCTATTTTTGCTGCCT
CCTGGTGGCGCAGCGAATGA

Product: sodium/proton antiporter

Products: Na (I) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: Sodium/proton antiporter nhaB [H]

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAGWLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMT
SAEHVREEVAANLEVLLLLMFMVAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTMVGEPQNLIIAKAAGWHFGDFFL
RMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKVREVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEV
GLIGLSVIILATSLTGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLFYIFNGLLS
SISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPNGQAAFLFLLTSALAPLIRLSYGRMVWMALP
YTLVLTLVGLLCVEFTLAPVTEWFMQMGWIATL

Sequences:

>Translated_513_residues
MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAGWLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMT
SAEHVREEVAANLEVLLLLMFMVAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTMVGEPQNLIIAKAAGWHFGDFFL
RMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKVREVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEV
GLIGLSVIILATSLTGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLFYIFNGLLS
SISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPNGQAAFLFLLTSALAPLIRLSYGRMVWMALP
YTLVLTLVGLLCVEFTLAPVTEWFMQMGWIATL
>Mature_513_residues
MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAGWLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMT
SAEHVREEVAANLEVLLLLMFMVAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTMVGEPQNLIIAKAAGWHFGDFFL
RMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKVREVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEV
GLIGLSVIILATSLTGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLFYIFNGLLS
SISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPNGQAAFLFLLTSALAPLIRLSYGRMVWMALP
YTLVLTLVGLLCVEFTLAPVTEWFMQMGWIATL

Specific function: Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons [H]

COG id: COG3067

COG function: function code P; Na+/H+ antiporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaB Na(+)/H(+) (TC 2.A.34) antiporter family [H]

Homologues:

Organism=Escherichia coli, GI1787435, Length=513, Percent_Identity=100, Blast_Score=1030, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004671 [H]

Pfam domain/function: PF06450 NhaB [H]

EC number: NA

Molecular weight: Translated: 56729; Mature: 56729

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3 Proton [Periplasm]; Na (I) [Cytoplasm] [C]

Specific reaction: 3 Proton [Periplasm] + 2 Na (I) [Cytoplasm] = 2 Na (I) [Periplasm] + 3 Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA