Click here to switch to the map view.

The map label for this gene is ycgF

Identifier: 157160663

GI number: 157160663

Start: 1261482

End: 1262693

Strand: Reverse

Name: ycgF

Synonym: EcHS_A1259

Alternate gene names: 157160663

Gene position: 1262693-1261482 (Counterclockwise)

Preceding gene: 157160664

Following gene: 157160662

Centisome position: 27.19

GC content: 43.56

Gene sequence:

>1212_bases
ATGCTTACCACCCTTATTTATCGTAGCCATATACGTGACGACGAACCTGTCAAAAAAATCGAAGAAATGGTTTCGATAGC
AAATCGCAGGAACATGCAGTCTGACGTAACAGGGATCTTACTGTTTAATGGTTCTCATTTTTTCCAGCTTCTGGAAGGTC
CGGAAGAACAGGTTAAAATGATATATCGGGCTATATGCCAGGATCCACGGCACTATAATATTGTTGAGCTGCTGTGCGAT
TACGCGCCTGCTCGCCGTTTTGGCAAAGCGGGAATGGAATTATTTGATTTGCGCCTGCACGAGCGAGATGACGTTTTACA
GGCCGTATTCGACAAAGGCACATCAAAATTTCAGCTAACTTATGATGACAGAGCGCTACAATTTTTTCGTACTTTTGTCC
TTGCAACCGAACAATCAACCTATTTCGAGATCCCTGCCGAAGACTCCTGGCTTTTTATCGCTGACGGATCTGATAAAGAA
CTTGATTCCTGTGCCCTTTCACCAACTATAAACGACCACTTTGCCTTTCATCCTATTGTCGATCCCTTATCGCGGCGGAT
AATCGCTTTTGAAGCCATTGTGCAAAAAAATGAAGATAGCCCATCAGCCATAGCGGTTGGGCAGCGTAAAGACGGGGAAA
TCTACACAGCGGATCTCAAAAGTAAGGCGCTTGCATTCACGATGGCACACGCACTTGAGCTCGGTGATAAAATGATTTCA
ATCAATCTATTACCTATGACCCTGGTTAACGAACCTGACGCAGTCTCTTTTTTACTTAATGAAATAAAGGCCAATGCTCT
GGTGCCTGAACAAATCATCGTTGAATTTACTGAAAGTGAAGTCATATCTCGGTTTGATGAGTTTGCCGAAGCGATTAAAT
CGCTAAAGGCTGCTGGTATCAGTGTAGCAATTGATCATTTTGGCGCAGGTTTTGCTGGTTTGTTACTCCTGTCACGCTTC
CAGCCTGACAGAATTAAAATCAGTCAGGAATTGATTACCAATGTTCATAAAAGTGGGCCACGGCAGGCAATTATTCAGGC
GATCATAAAATGCTGTACATCACTTGAAATTCAAGTCAGTGCTATGGGCGTGGCAACACCAGAAGAGTGGATGTGGCTTG
AATCCGCAGGAATTGAGATGTTTCAGGGAGATCTGTTTGCTAAAGCTAAATTGAATGGTATCCCTTCCATAGCGTGGCCA
GAGAAAAAATAA

Upstream 100 bases:

>100_bases
AATGTAAAGAAACGGTTATGTTTTGGTATTAAAAGAAAGACACAAGAAGATATGTCTGTTACCGTCTTACTCTCGCCTCA
CCCATTACCCTGGATTGTTA

Downstream 100 bases:

>100_bases
TTTTCAGCACATTCTTTCACATGATTTCAGTAAATCATCGTGAAAATGAATACGTTACGTTTAAATTTAATCCACATTAA
AAATAATGTTTCATCCAATT

Product: BLUF/cyclic diguanylate phosphodiesterase domain-containing protein

Products: NA

Alternate protein names: Blrp

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNGSHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCD
YAPARRFGKAGMELFDLRLHERDDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
LDSCALSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTADLKSKALAFTMAHALELGDKMIS
INLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEFTESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRF
QPDRIKISQELITNVHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLNGIPSIAWP
EKK

Sequences:

>Translated_403_residues
MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNGSHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCD
YAPARRFGKAGMELFDLRLHERDDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
LDSCALSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTADLKSKALAFTMAHALELGDKMIS
INLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEFTESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRF
QPDRIKISQELITNVHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLNGIPSIAWP
EKK
>Mature_403_residues
MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNGSHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCD
YAPARRFGKAGMELFDLRLHERDDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
LDSCALSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTADLKSKALAFTMAHALELGDKMIS
INLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEFTESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRF
QPDRIKISQELITNVHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLNGIPSIAWP
EKK

Specific function: Binds to and releases the YcgE repressor from its bound DNA target in a blue-light-dependent (470 nm) fashion. A shift to low temperature also triggers a YcgF-medisted relief of repression by YcgE, suggesting YcgF may serve as a thermometer. Blue light ma

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain

Homologues:

Organism=Escherichia coli, GI1787410, Length=403, Percent_Identity=100, Blast_Score=831, Evalue=0.0,
Organism=Escherichia coli, GI1787541, Length=129, Percent_Identity=37.2093023255814, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790496, Length=225, Percent_Identity=28, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI226510982, Length=153, Percent_Identity=32.6797385620915, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1788502, Length=226, Percent_Identity=25.2212389380531, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1789650, Length=121, Percent_Identity=26.4462809917355, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082096, Length=167, Percent_Identity=25.748502994012, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YCGF_ECOLI (P75990)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- PIR:   H64861
- RefSeq:   AP_001788.1
- RefSeq:   NP_415681.1
- ProteinModelPortal:   P75990
- SMR:   P75990
- DIP:   DIP-11553N
- STRING:   P75990
- EnsemblBacteria:   EBESCT00000001868
- EnsemblBacteria:   EBESCT00000014459
- GeneID:   947592
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1150
- KEGG:   eco:b1163
- EchoBASE:   EB3646
- EcoGene:   EG13887
- eggNOG:   COG2200
- GeneTree:   EBGT00050000008779
- HOGENOM:   HBG467761
- OMA:   AEEWMWL
- ProtClustDB:   CLSK891732
- BioCyc:   EcoCyc:G6603-MONOMER
- Genevestigator:   P75990
- InterPro:   IPR001792
- InterPro:   IPR007024
- InterPro:   IPR001633
- SMART:   SM00052

Pfam domain/function: PF04940 BLUF; PF00563 EAL; SSF54975 Acylphosphatase

EC number: NA

Molecular weight: Translated: 45296; Mature: 45296

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS50925 BLUF; PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNGSHFFQLLEGPEEQVKM
CCHHHEEHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHCCCHHHHHH
IYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHERDDVLQAVFDKGTSKFQLT
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHCCCCEEEEE
YDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKELDSCALSPTINDHFAFHPIV
ECHHHHHHHHHHHHEECCCCEEECCCCCCEEEEECCCCCCHHHEECCCCCCCCCEECCHH
DPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTADLKSKALAFTMAHALELGDKMIS
HHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCEEEECHHHHHHHHHHHHHHHHCCEEEE
INLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEFTESEVISRFDEFAEAIKSLKAAGI
EEEEEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SVAIDHFGAGFAGLLLLSRFQPDRIKISQELITNVHKSGPRQAIIQAIIKCCTSLEIQVS
EEEEHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEE
AMGVATPEEWMWLESAGIEMFQGDLFAKAKLNGIPSIAWPEKK
EEECCCCHHHEEEHHCCCHHHCCCEEEEHHCCCCCCCCCCCCC
>Mature Secondary Structure
MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNGSHFFQLLEGPEEQVKM
CCHHHEEHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHCCCHHHHHH
IYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHERDDVLQAVFDKGTSKFQLT
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHCCCCEEEEE
YDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKELDSCALSPTINDHFAFHPIV
ECHHHHHHHHHHHHEECCCCEEECCCCCCEEEEECCCCCCHHHEECCCCCCCCCEECCHH
DPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTADLKSKALAFTMAHALELGDKMIS
HHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCEEEECHHHHHHHHHHHHHHHHCCEEEE
INLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEFTESEVISRFDEFAEAIKSLKAAGI
EEEEEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SVAIDHFGAGFAGLLLLSRFQPDRIKISQELITNVHKSGPRQAIIQAIIKCCTSLEIQVS
EEEEHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEE
AMGVATPEEWMWLESAGIEMFQGDLFAKAKLNGIPSIAWPEKK
EEECCCCHHHEEEHHCCCHHHCCCEEEEHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503