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The map label for this gene is mlrA

Identifier: 157160662

GI number: 157160662

Start: 1260546

End: 1261277

Strand: Reverse

Name: mlrA

Synonym: EcHS_A1258

Alternate gene names: 157160662

Gene position: 1261277-1260546 (Counterclockwise)

Preceding gene: 157160663

Following gene: 157160661

Centisome position: 27.16

GC content: 48.77

Gene sequence:

>732_bases
GTGGCTTATTACAGCATTGGTGATGTTGCTGAACGTTGCGGGATTAATCCTGTCACTCTCCGGGCCTGGCAACGCCGCTA
CGGTTTGTTAAAACCACAGCGCAGTGAAGGCGGACACCGACTCTTTGATGAAGAAGACATACAACGCATCGAAGAGATCA
AGCGTTGGATAAGTAATGGCGTCCCTGTAGGCAAAGTTAAAGCATTACTGGAAACCACCAGCCAGGATACGGAAGATGAC
TGGAGCCGCCTGCAAGAAGAGATGATGTCAATTCTTCGCATGGCTAATCCTGCCAAACTACGCGCGAGAATTATTTCACT
GGGTCGAGAGTACCCAGTAGATCAATTGATTAATCATGTTTACCTTCCTGTTCGCCAGCGTCTCGTGCTTGATCACAACA
CCTCCCGCATTATGAGCAGTATGTTTGACGGCGCATTAATTGAATACGCAGCAACCTCGCTTTTCGAAATGCGCCGTAAG
CCCGGTAAAGAAGCCATTCTGATGGCGTGGAATGTTGAAGAGAGAGCACGATTGTGGCTGGAAGCATGGCGTTTATCATT
GTCAGGATGGCACATTTCTGTTCTTGCTGATCCCATTGAATCGCCGCGCCCGGAACTGTTCCCGACGCAAACATTGATTG
TCTGGACAGGCATGGCACCAACGAGAAGGCAAAACGAACTTTTACAACACTGGGGTGAGCAAGGTTATAAAGTCATCTTT
CATGCCCCCTAA

Upstream 100 bases:

>100_bases
TGAAATCTTATTTATACTTTGTACAATTTTAGCGTATATTGTACAAAGTATTATTGGGTCGTGTACAGGCGACGGAGATT
TGTGACCGCAAGGAGGAATT

Downstream 100 bases:

>100_bases
TTTCACCTTTATCCCGCCCCTTAGACAGAATGTTATAATCTGAACCCACAACAGCGTTTGTACCGGAGCTGGTCCGTTGC
AAAATGCTCACCGGGAGAAT

Product: transcriptional regulator mlrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDEEDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDD
WSRLQEEMMSILRMANPAKLRARIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGMAPTRRQNELLQHWGEQGYKVIF
HAP

Sequences:

>Translated_243_residues
MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDEEDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDD
WSRLQEEMMSILRMANPAKLRARIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGMAPTRRQNELLQHWGEQGYKVIF
HAP
>Mature_242_residues
AYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDEEDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDW
SRLQEEMMSILRMANPAKLRARIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRKP
GKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGMAPTRRQNELLQHWGEQGYKVIFH
AP

Specific function: Controls the expression of 6 small proteins that may play a role in biofilm maturation. Binds to and represses the operator of the ycgZ-ymgA-ariR-ymgC operon, it also regulates yliL and ynaK. Binding is antagonized by YcgF upon blue-light (470 nm) irradia

COG id: COG0789

COG function: function code K; Predicted transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH merR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1787409, Length=243, Percent_Identity=100, Blast_Score=499, Evalue=1e-143,
Organism=Escherichia coli, GI1788448, Length=239, Percent_Identity=48.5355648535565, Blast_Score=242, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YCGE_ECOLI (P75989)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- PIR:   G64861
- RefSeq:   AP_001787.1
- RefSeq:   NP_415680.1
- ProteinModelPortal:   P75989
- SMR:   P75989
- DIP:   DIP-11552N
- IntAct:   P75989
- MINT:   MINT-1244002
- STRING:   P75989
- EnsemblBacteria:   EBESCT00000000225
- EnsemblBacteria:   EBESCT00000017210
- GeneID:   945735
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1149
- KEGG:   eco:b1162
- EchoBASE:   EB3645
- EcoGene:   EG13886
- eggNOG:   COG0789
- GeneTree:   EBGT00050000010907
- HOGENOM:   HBG417247
- OMA:   DRTRLWL
- ProtClustDB:   CLSK2301693
- BioCyc:   EcoCyc:G6602-MONOMER
- Genevestigator:   P75989
- InterPro:   IPR009061
- InterPro:   IPR000551
- SMART:   SM00422

Pfam domain/function: PF00376 MerR; SSF46955 Putativ_DNA_bind

EC number: NA

Molecular weight: Translated: 28265; Mature: 28134

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS00552 HTH_MERR_1; PS50937 HTH_MERR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDEEDIQRIEEIKRWISNG
CCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
VPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRARIISLGREYPVDQLINHV
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHH
YLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRKPGKEAILMAWNVEERARLWL
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHH
EAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGMAPTRRQNELLQHWGEQGYKVIF
HHHHHHHCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEEE
HAP
ECC
>Mature Secondary Structure 
AYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDEEDIQRIEEIKRWISNG
CCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
VPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRARIISLGREYPVDQLINHV
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHH
YLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRKPGKEAILMAWNVEERARLWL
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHH
EAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGMAPTRRQNELLQHWGEQGYKVIF
HHHHHHHCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEEE
HAP
ECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503