Click here to switch to the map view.

The map label for this gene is mdtG

Identifier: 157160579

GI number: 157160579

Start: 1177937

End: 1179166

Strand: Reverse

Name: mdtG

Synonym: EcHS_A1175

Alternate gene names: 157160579

Gene position: 1179166-1177937 (Counterclockwise)

Preceding gene: 157160581

Following gene: 157160578

Centisome position: 25.39

GC content: 52.28

Gene sequence:

>1230_bases
ATGTCACCCTGTGAAAATGACACCCCTATAAACTGGAAACGAAACCTGATCGTCGCCTGGCTAGGCTGTTTTCTTACCGG
GGCCGCCTTCAGTCTGGTAATGCCCTTCTTACCCCTCTACGTTGAGCAGCTTGGCGTTACCGGTCACTCCGCCCTGAATA
TGTGGTCCGGTATTGTCTTCAGCATTACATTTTTATTTTCGGCCATCGCCTCACCGTTTTGGGGTGGACTCGCCGACCGT
AAAGGCCGAAAACTCATGCTATTACGCTCTGCTCTCGGCATGGGCATCGTAATGGTGTTGATGGGACTGGCACAAAATAT
CTGGCAGTTTTTGATCCTACGGGCGCTTCTTGGGTTACTTGGCGGATTTGTCCCCAACGCTAATGCTCTTATCGCCACAC
AAGTACCGCGTAATAAAAGCGGCTGGGCGCTGGGTACGCTCTCCACAGGCGGCGTTAGTGGTGCGTTGCTCGGCCCAATG
GCTGGCGGCCTGCTCGCCGATAGCTACGGCTTACGTCCGGTATTCTTTATTACCGCCAGTGTGCTCATACTCTGCTTTTT
CGTCACCCTGTTTTGCATCAGAGAAAAATTCCAGCCGGTCAGCAAAAAAGAGATGCTGCACATGCGGGAAGTGGTGACAT
CACTTAAAAACCCGAAACTGGTACTCAGCCTGTTTGTCACTACGTTAATCATCCAGGTGGCGACGGGCTCAATTGCCCCC
ATTCTGACGCTGTATGTCCGCGAACTGGCGGGTAACGTCAGTAACGTCGCCTTTATCAGTGGCATGATCGCCTCGGTGCC
AGGCGTGGCGGCTCTGCTGCTAAGTGCACCACGACTCGGCAAACTTGGCGATCGAATCGGACCCGAAAAGATCCTGATTA
CAGCGCTGATCTTTTCTGTACTGCTGTTGATCCCAATGTCTTACGTTCAGACGCCATTGCAACTTGGGATTTTACGTTTT
TTGCTCGGTGCCGCCGATGGTGCACTACTCCCCGCCGTACAGACACTGTTGGTTTACAACTCGAGCAACCAGATCGCCGG
GCGTATCTTCAGCTATAACCAATCGTTTCGTGATATTGGCAACGTTACCGGACCATTGATGGGAGCAGCGATTTCAGCGA
ACTACGGTTTCAGAGCGGTATTTCTCGTCACCGCTGGCGTAGTGTTATTCAACGCAGTCTATTCATGGAACAGTCTACGT
CGTCGTCGAATACCCCAGGTATCGAACTGA

Upstream 100 bases:

>100_bases
AGCTCTCTGGATTGCGCCCCCTGGAAGTCGGGCGCATAATTAGTGTGCTTATCTTTTCTTCTTATGTTCACCGCGCCTGG
CGCACCAACAGCGGATTGCT

Downstream 100 bases:

>100_bases
TTTTTCGCCTTTCATACTTGCAAAAGCGGAGAATCAGCTATCCTTTTCCCTGAAACCTCATCAACTCAAAGGGAGAATCG
TGATGACCATGTACGCAACG

Product: drug efflux system protein MdtG

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQLGVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADR
KGRKLMLLRSALGMGIVMVLMGLAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNPKLVLSLFVTTLIIQVATGSIAP
ILTLYVRELAGNVSNVAFISGMIASVPGVAALLLSAPRLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRF
LLGAADGALLPAVQTLLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAVYSWNSLR
RRRIPQVSN

Sequences:

>Translated_409_residues
MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQLGVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADR
KGRKLMLLRSALGMGIVMVLMGLAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNPKLVLSLFVTTLIIQVATGSIAP
ILTLYVRELAGNVSNVAFISGMIASVPGVAALLLSAPRLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRF
LLGAADGALLPAVQTLLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAVYSWNSLR
RRRIPQVSN
>Mature_408_residues
SPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQLGVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRK
GRKLMLLRSALGMGIVMVLMGLAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPMA
GGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNPKLVLSLFVTTLIIQVATGSIAPI
LTLYVRELAGNVSNVAFISGMIASVPGVAALLLSAPRLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFL
LGAADGALLPAVQTLLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAVYSWNSLRR
RRIPQVSN

Specific function: Confers resistance to fosfomycin and deoxycholate

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. MdtG (TC 2.A.1.2.20) subfamily

Homologues:

Organism=Escherichia coli, GI1787291, Length=409, Percent_Identity=99.7555012224939, Blast_Score=777, Evalue=0.0,
Organism=Escherichia coli, GI87082404, Length=303, Percent_Identity=24.7524752475248, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319871, Length=153, Percent_Identity=29.4117647058824, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MDTG_ECOHS (A7ZZ10)

Other databases:

- EMBL:   CP000802
- RefSeq:   YP_001457897.1
- ProteinModelPortal:   A7ZZ10
- STRING:   A7ZZ10
- EnsemblBacteria:   EBESCT00000053699
- GeneID:   5595064
- GenomeReviews:   CP000802_GR
- KEGG:   ecx:EcHS_A1175
- eggNOG:   COG0477
- GeneTree:   EBGT00050000008819
- HOGENOM:   HBG404070
- OMA:   FIHENFL
- ProtClustDB:   PRK09874
- HAMAP:   MF_01528
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001958
- PRINTS:   PR01035

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 43981; Mature: 43850

Theoretical pI: Translated: 10.85; Mature: 10.85

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1416bedc)-; HASH(0x130c7570)-; HASH(0x1402ca10)-; HASH(0x1356a10c)-; HASH(0x13cacb4c)-; HASH(0x13db5068)-; HASH(0x1357d788)-; HASH(0x1413a198)-; HASH(0x1442059c)-; HASH(0xfbb4a74)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQLGVTGHSALNMWSGIVF
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
SITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMGLAQNIWQFLILRALLGLL
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPMAGGLLADSYGLRPVFFITAS
CCCCCCCCEEEEEECCCCCCCCEEEECCCCCCCHHHHHHHHCCHHHCCCCCCHHHHHHHH
VLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNPKLVLSLFVTTLIIQVATGSIAP
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHH
ILTLYVRELAGNVSNVAFISGMIASVPGVAALLLSAPRLGKLGDRIGPEKILITALIFSV
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHH
LLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQTLLVYNSSNQIAGRIFSYNQSFRDIG
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHH
NVTGPLMGAAISANYGFRAVFLVTAGVVLFNAVYSWNSLRRRRIPQVSN
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
>Mature Secondary Structure 
SPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQLGVTGHSALNMWSGIVF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
SITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMGLAQNIWQFLILRALLGLL
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPMAGGLLADSYGLRPVFFITAS
CCCCCCCCEEEEEECCCCCCCCEEEECCCCCCCHHHHHHHHCCHHHCCCCCCHHHHHHHH
VLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNPKLVLSLFVTTLIIQVATGSIAP
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHH
ILTLYVRELAGNVSNVAFISGMIASVPGVAALLLSAPRLGKLGDRIGPEKILITALIFSV
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHH
LLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQTLLVYNSSNQIAGRIFSYNQSFRDIG
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHH
NVTGPLMGAAISANYGFRAVFLVTAGVVLFNAVYSWNSLRRRRIPQVSN
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA