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The map label for this gene is htrB

Identifier: 157160581

GI number: 157160581

Start: 1179338

End: 1180258

Strand: Reverse

Name: htrB

Synonym: EcHS_A1177

Alternate gene names: 157160581

Gene position: 1180258-1179338 (Counterclockwise)

Preceding gene: 157160583

Following gene: 157160579

Centisome position: 25.42

GC content: 53.2

Gene sequence:

>921_bases
ATGACGAATCTACCCAAGTTCTCCACCGCACTGCTTCATCCGCGTTATTGGTTAACCTGGTTGGGTATTGGCGTACTTTG
GTTAGTCGTGCAATTGCCCTACCCGGTTATCTACCGCCTCGGTTGTGGATTAGGAAAACTGGCGTTACGTTTTATGAAAC
GACGCGCAAAAATTGTGCATCGCAACCTGGAACTGTGCTTCCCGGAAATGAGCGAACAAGAACGCCGTAAAATGGTGGTG
AAGAATTTCGAATCCGTTGGCATGGGCCTGATGGAAACCGGCATGGCGTGGTTCTGGCCGGACCGCCGAATCGCCCGCTG
GACGGAAGTGATCGGCATGGAACACATTCGTGACGTGCAGGCGCAAAAACGCGGCATCCTGTTAGTTGGCATCCATTTTC
TGACACTGGAGCTGGGTGCGCGGCAGTTTGGTATGCAGGAACCGGGGATTGGCGTTTACCGCCCGAACGATAATCCACTG
ATTGACTGGCTACAAACCTGGGGCCGTTTGCGCTCAAATAAATCGATGCTCGACCGCAAAGATTTAAAAGGCATGATTAA
AGCCCTGAAAAAAGGCGAAGTGGTCTGGTACGCACCGGATCATGATTACGGCCCGCGCTCAAGCGTTTTCGTCCCGTTGT
TTGCCGTTGAGCAGGCTGCGACCACGACCGGAACCTGGATGCTGGCACGGATGTCCGGCGCATGTCTGGTGCCCTTCGTT
CCACGCCGTAAGCCAGATGGCAAAGGGTATCAATTGATTATGCTGCCGCCAGAGTGTTCTCCGCCACTGGATGATGCCGA
AACTACCGCCGCGTGGATGAACAAAGTGGTCGAAAAATGCATCATGATGGCACCAGAGCAGTATATGTGGTTACACCGTC
GCTTTAAAACACGCCCGGAAGGCGTTCCTTCACGCTATTAA

Upstream 100 bases:

>100_bases
GCGGCAATTTCGCCCAGTCTTCAGCCACAAATTTTGGTTGCGGGCGAAAAAATGCGACAATACATACAATTGCCCGAATA
GGTTGAAAAACAGGATTGAT

Downstream 100 bases:

>100_bases
ATCTCCCATGCCGGATGCTTCAGAATGGCATCCGGCATTACCACAGCAAATCCCCCTGATTTAGCGATAAAAGCTCTCTG
GATTGCGCCCCCTGGAAGTC

Product: lipid A biosynthesis lauroyl acyltransferase

Products: NA

Alternate protein names: Heat shock protein B

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGLGKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVV
KNFESVGMGLMETGMAWFWPDRRIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQAATTTGTWMLARMSGACLVPFV
PRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKVVEKCIMMAPEQYMWLHRRFKTRPEGVPSRY

Sequences:

>Translated_306_residues
MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGLGKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVV
KNFESVGMGLMETGMAWFWPDRRIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQAATTTGTWMLARMSGACLVPFV
PRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKVVEKCIMMAPEQYMWLHRRFKTRPEGVPSRY
>Mature_305_residues
TNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGLGKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVK
NFESVGMGLMETGMAWFWPDRRIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPLI
DWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQAATTTGTWMLARMSGACLVPFVP
RRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKVVEKCIMMAPEQYMWLHRRFKTRPEGVPSRY

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family

Homologues:

Organism=Escherichia coli, GI1787292, Length=306, Percent_Identity=100, Blast_Score=632, Evalue=0.0,
Organism=Escherichia coli, GI87082094, Length=301, Percent_Identity=54.8172757475083, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1788161, Length=310, Percent_Identity=30.6451612903226, Blast_Score=121, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HTRB_ECO57 (P0ACV1)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D85667
- PIR:   H90807
- RefSeq:   NP_287188.1
- RefSeq:   NP_309459.1
- ProteinModelPortal:   P0ACV1
- EnsemblBacteria:   EBESCT00000025123
- EnsemblBacteria:   EBESCT00000059909
- GeneID:   912457
- GeneID:   959388
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z1690
- KEGG:   ecs:ECs1432
- GeneTree:   EBGT00050000010320
- HOGENOM:   HBG730280
- OMA:   TIWYAPD
- ProtClustDB:   PRK06860
- BioCyc:   ECOL83334:ECS1432-MONOMER
- InterPro:   IPR004960
- InterPro:   IPR011920
- TIGRFAMs:   TIGR02207

Pfam domain/function: PF03279 Lip_A_acyltrans

EC number: 2.3.1.-

Molecular weight: Translated: 35407; Mature: 35276

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1401da50)-;

Cys/Met content:

1.6 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGLGKLALRFMKRRAKIVH
CCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHH
RNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDRRIARWTEVIGMEHIRDVQ
HHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHH
AQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPLIDWLQTWGRLRSNKSMLDRK
HHHCCEEEEEHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHCCHHHHHHH
DLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQAATTTGTWMLARMSGACLVPFV
HHHHHHHHHHCCCEEEECCCCCCCCCCCEEEEHHHHHHHHCCHHHHHHHHHCCCEEECCC
PRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKVVEKCIMMAPEQYMWLHRRFKTRPE
CCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
GVPSRY
CCCCCH
>Mature Secondary Structure 
TNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGLGKLALRFMKRRAKIVH
CCCCCHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHH
RNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDRRIARWTEVIGMEHIRDVQ
HHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHH
AQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPLIDWLQTWGRLRSNKSMLDRK
HHHCCEEEEEHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHCCHHHHHHH
DLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQAATTTGTWMLARMSGACLVPFV
HHHHHHHHHHCCCEEEECCCCCCCCCCCEEEEHHHHHHHHCCHHHHHHHHHCCCEEECCC
PRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKVVEKCIMMAPEQYMWLHRRFKTRPE
CCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
GVPSRY
CCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796