| Definition | Escherichia coli HS, complete genome. |
|---|---|
| Accession | NC_009800 |
| Length | 4,643,538 |
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The map label for this gene is pgaB [H]
Identifier: 157160547
GI number: 157160547
Start: 1150138
End: 1152156
Strand: Reverse
Name: pgaB [H]
Synonym: EcHS_A1138
Alternate gene names: 157160547
Gene position: 1152156-1150138 (Counterclockwise)
Preceding gene: 157160548
Following gene: 157160546
Centisome position: 24.81
GC content: 44.48
Gene sequence:
>2019_bases ATGTTACGTAATGGAAATAAATATCTCCTGATGCTGGTGAGTATAATTATGCTCACCGCGTGCATTAGCCAGTCAAGAAC ATCATTTATACCGCCACAGGATCGCGAATCTTTACTCGCCGAGCAACCGTGGCCGCATAATGGTTTTGTAGCGATTTCAT GGCATAACGTTGAAGACGAAGCTGCCGACCAGCGTTTTATGTCAGTGCGGACATCAGCACTGCGTGAACAATTTGCCTGG CTGCGCGAGAACGGTTATCAACCGGTCAGTATTGCTCAAATTCGTGAAGCACGTCGAGGAGGAAAACCGCTACCGGAAAA AGCTGTAGTGCTGACTTTTGATGACGGCTACCAGAGTTTTTATACCCGCGTCTTCCCAATTCTTCAGGCCTTCCAGTGGC CTGCTGTATGGGCCCCCGTCGGCAGTTGGGTCGATACGCCAGCGGATAAACAAGTAAAATTTGGCGATGAGTTGGTCGAT CGAGAATATTTTGCCACGTGGCAACAAGTGCGAGAAGTTGCGCGTTCCCGGCTCGTTGAGCTCGCTTCTCATACATGGAA TTCTCACTACGGTATTCAGGCTAATGCCACCGGCAGCTTATTGCCTGTATATGTAAATCGTGCATATTTTACTGACCACG CACGGTATGAAACCGCAGCAGAATACCGGGAAAGAATTCGTCTGGATGCTGTAAAAATGACGGAATACCTGCGTACAAAG GTTGAGGTAAATCCACACGTTTTTGTTTGGCCTTATGGCGAAGCGAATGGCATAGCGATAGAGGAATTAAAAAAACTCGG TTATGACATGTTCTTCACCCTTGAATCAGGTTTGGCAAATGCGTCGCAATTGGATTCCATTCCGCGGGTATTAATCGCCA ATAATCCCTCATTAAAAGAGTTTGCCCAGCAAATTATTACCGTACAGGAAAAATCACCACAACGGATAATGCATATCGAT CTTGATTACGTTTATGACGAAAACCTCCAGCAAATGGATCGCAATATTGATGTGCTAATTCAGCGGGTGAAAGATATGCA AATATCAACCGTGTATTTGCAGGCATTTGCTGATCCCGATGGTGATGGGCTGGTCAAAGAGGTCTGGTTTCCAAATCGTT TGCTACCAATGAAAGCAGATATTTTTAGTCGGGTTGCCTGGCAATTACGTACCCGCTCAGGTGTAAACATCTATGCGTGG ATGCCGGTATTAAGCTGGGATTTAGATCCCACATTAACGCGAGTAAAATACTTACCAACAGGGGAGAAAAAAGCACAAAT TCATCCTGAACAATATCACCGTCTCTCTCCTTTCGATGACAGAGTCAGAGCACAAGTTGGCATGTTATATGAAGATCTTG CCGGACATGCTGCTTTTGATGGCATATTGTTCCACGATGATGCTTTGCTTTCAGATTATGAAGATGCCAGTGCACCGGCT ATCACGGCTTATCAGCAAGCAGGCTTTAGCGGGAGTCTGAGCGAAATTCGACAAAACCCGGAGCAATTTAAACAGTGGGC CCGCTTTAAAAGTCGTGCGTTAACTGACTTCACTTTAGAACTTAGTGCGCGCGTAAAAGCCATTCGCGGTCCACATATTA AAACTGCACGAAATATTTTTGCACTTCCGGTAATACAACCTGAAAGTGAAGCCTGGTTTGCACAGAATTATGCTGATTTC CTAAAAAGCTATGACTGGACCGCTATTATGGCTATGCCTTATCTGGAAGGTGTCGCAGAAAAATCGGCTGACCAATGGTT AATACAATTGACCAATCAAATTAAAAACATCCCTCAGGCTAAAGACAAATCTATTTTAGAATTACAGGCACAAAACTGGC AGAAAAATGGTCAGCATCAGGCTATTTCTTCGCAACAACTCGCTCACTGGATGAGCCTATTACAACTGAATGGAGTGAAA AACTATGGTTATTATCCCGACAATTTTCTGCATAACCAACCTGAAATAGACCTTATTCGTCCTGAGTTTTCAACAGCCTG GTATCCGAAAAATGATTAA
Upstream 100 bases:
>100_bases CTGGCGCAACGCTACGCTGGGAAAAACGACCTTATGACGGTGACAGAGAACACAACTTATACGTTGAATTCGATATGACA TTCAGATTTTAAGGATAAAT
Downstream 100 bases:
>100_bases TCGCATCGTATCGTTTTTTATATTATGTCTGGTGTTATGCATACCCCTATGCGTAGCGTACTTTCACTCTGGTGAACTGA TGATGAGGTTCGTTTTCTTC
Product: biofilm PGA synthesis lipoprotein pgaB
Products: NA
Alternate protein names: PGA N-deacetylase; Poly-beta-1,6-GlcNAc N-deacetylase [H]
Number of amino acids: Translated: 672; Mature: 672
Protein sequence:
>672_residues MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPWPHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAW LRENGYQPVSIAQIREARRGGKPLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYETAAEYRERIRLDAVKMTEYLRTK VEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLESGLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHID LDYVYDENLQQMDRNIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTRSGVNIYAW MPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLYEDLAGHAAFDGILFHDDALLSDYEDASAPA ITAYQQAGFSGSLSEIRQNPEQFKQWARFKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADF LKSYDWTAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISSQQLAHWMSLLQLNGVK NYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND
Sequences:
>Translated_672_residues MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPWPHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAW LRENGYQPVSIAQIREARRGGKPLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYETAAEYRERIRLDAVKMTEYLRTK VEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLESGLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHID LDYVYDENLQQMDRNIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTRSGVNIYAW MPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLYEDLAGHAAFDGILFHDDALLSDYEDASAPA ITAYQQAGFSGSLSEIRQNPEQFKQWARFKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADF LKSYDWTAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISSQQLAHWMSLLQLNGVK NYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND >Mature_672_residues MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPWPHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAW LRENGYQPVSIAQIREARRGGKPLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYETAAEYRERIRLDAVKMTEYLRTK VEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLESGLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHID LDYVYDENLQQMDRNIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTRSGVNIYAW MPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLYEDLAGHAAFDGILFHDDALLSDYEDASAPA ITAYQQAGFSGSLSEIRQNPEQFKQWARFKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADF LKSYDWTAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISSQQLAHWMSLLQLNGVK NYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND
Specific function: Catalyzes the N-deacetylation of poly-beta-1,6-N-acetyl- D-glucosamine (PGA), a biofilm adhesin polysaccharide. N- deacetylation promotes PGA export through the PgaA porin [H]
COG id: COG0726
COG function: function code G; Predicted xylanase/chitin deacetylase
Gene ontology:
Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the polysaccharide deacetylase family [H]
Homologues:
Organism=Escherichia coli, GI1787260, Length=672, Percent_Identity=99.8511904761905, Blast_Score=1388, Evalue=0.0,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR011330 - InterPro: IPR002509 [H]
Pfam domain/function: PF01522 Polysacc_deac_1 [H]
EC number: NA
Molecular weight: Translated: 77433; Mature: 77433
Theoretical pI: Translated: 6.14; Mature: 6.14
Prosite motif: PS00013 PROKAR_LIPOPROTEIN
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.1 %Cys (Translated Protein) 2.2 %Met (Translated Protein) 2.4 %Cys+Met (Translated Protein) 0.1 %Cys (Mature Protein) 2.2 %Met (Mature Protein) 2.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPWPHNGFVAISWHNVEDE CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCEEEEEECCCCHH AADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREARRGGKPLPEKAVVLTFDDGYQSF HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCHHHH YTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVDREYFATWQQVREVARSRLVE HHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH LASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYETAAEYRERIRLDAVKMTEYLRTK HHHHCCCCCCCEEECCCCCEEEEEEEEEHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH VEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLESGLANASQLDSIPRVLIANNPSLKE EECCCEEEEEECCCCCCCCHHHHHHCCCEEEEEEHHCCCCHHHHHCCCEEEEECCCCHHH FAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDRNIDVLIQRVKDMQISTVYLQAFADPD HHHHHHHHCCCCCCEEEEEEEHHHHCCCHHHHHCCHHHHHHHHHCCEEEEEEEEEECCCC GDGLVKEVWFPNRLLPMKADIFSRVAWQLRTRSGVNIYAWMPVLSWDLDPTLTRVKYLPT CCCCHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHEEECCC GEKKAQIHPEQYHRLSPFDDRVRAQVGMLYEDLAGHAAFDGILFHDDALLSDYEDASAPA CCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHCCEEEECCHHHCCCCCCCCCE ITAYQQAGFSGSLSEIRQNPEQFKQWARFKSRALTDFTLELSARVKAIRGPHIKTARNIF EHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHCCHHEEEEHHHHHHHHCCCCCHHHCCEE ALPVIQPESEAWFAQNYADFLKSYDWTAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQA EEEEECCCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC KDKSILELQAQNWQKNGQHQAISSQQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIR CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEC PEFSTAWYPKND CCCCCCCCCCCC >Mature Secondary Structure MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPWPHNGFVAISWHNVEDE CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCEEEEEECCCCHH AADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREARRGGKPLPEKAVVLTFDDGYQSF HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCHHHH YTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVDREYFATWQQVREVARSRLVE HHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH LASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYETAAEYRERIRLDAVKMTEYLRTK HHHHCCCCCCCEEECCCCCEEEEEEEEEHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH VEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLESGLANASQLDSIPRVLIANNPSLKE EECCCEEEEEECCCCCCCCHHHHHHCCCEEEEEEHHCCCCHHHHHCCCEEEEECCCCHHH FAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDRNIDVLIQRVKDMQISTVYLQAFADPD HHHHHHHHCCCCCCEEEEEEEHHHHCCCHHHHHCCHHHHHHHHHCCEEEEEEEEEECCCC GDGLVKEVWFPNRLLPMKADIFSRVAWQLRTRSGVNIYAWMPVLSWDLDPTLTRVKYLPT CCCCHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHEEECCC GEKKAQIHPEQYHRLSPFDDRVRAQVGMLYEDLAGHAAFDGILFHDDALLSDYEDASAPA CCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHCCEEEECCHHHCCCCCCCCCE ITAYQQAGFSGSLSEIRQNPEQFKQWARFKSRALTDFTLELSARVKAIRGPHIKTARNIF EHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHCCHHEEEEHHHHHHHHCCCCCHHHCCEE ALPVIQPESEAWFAQNYADFLKSYDWTAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQA EEEEECCCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC KDKSILELQAQNWQKNGQHQAISSQQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIR CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEC PEFSTAWYPKND CCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 11206551; 11258796 [H]