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The map label for this gene is pgaC [H]

Identifier: 157160546

GI number: 157160546

Start: 1148820

End: 1150145

Strand: Reverse

Name: pgaC [H]

Synonym: EcHS_A1137

Alternate gene names: 157160546

Gene position: 1150145-1148820 (Counterclockwise)

Preceding gene: 157160547

Following gene: 157160545

Centisome position: 24.77

GC content: 46.68

Gene sequence:

>1326_bases
ATGATTAATCGCATCGTATCGTTTTTTATATTATGTCTGGTGTTATGCATACCCCTATGCGTAGCGTACTTTCACTCTGG
TGAACTGATGATGAGGTTCGTTTTCTTCTGGCCGTTTTTTATGTCCATTATGTGGATTGTTGGCGGCGTCTATTTCTGGG
TCTATCGTGAACGCCACTGGCCGTGGGGAGAAAACGCACCAGCTCCCCAGTTGAAAGATAATCCGTCTATCTCCATTATC
ATTCCCTGTTTTAATGAGGAGAAAAACGTTGAGGAAACCATACACGCCGCTTTAGCACAGCGTTATGAGAACATTGAAGT
TATTGCCGTAAATGACGGTTCAACAGATAAAACCCGTGCCATCCTGGATCGCATGGCTGCACAAATTCCCCATTTGCGGG
TCATTCATCTGGCGCAAAACCAGGGGAAAGCCATTGCGCTTAAAACCGGAGCTGCCGCGGCGAAAAGTGAATATCTGGTG
TGCATTGATGGCGATGCGTTATTAGACCGCGATGCGGCGGCATATATTGTGGAACCGATGTTGTACAACCCGCGTGTGGG
TGCCGTAACCGGTAATCCTCGTATTCGAACACGTTCTACCCTGGTGGGTAAAATTCAGGTTGGCGAGTATTCCTCAATTA
TTGGTTTGATCAAGCGAACCCAGCGTATCTATGGAAACGTATTTACCGTTTCCGGTGTTATTGCCGCATTTCGTCGCAGC
GCCCTGGCAGAAGTGGGTTACTGGAGTGACGATATGATCACCGAAGATATTGATATTAGCTGGAAGCTGCAGTTGAATCA
GTGGACGATTTTTTACGAGCCACGGGCACTGTGCTGGATATTAATGCCTGAAACGTTAAAAGGGCTGTGGAAACAGCGCC
TGCGCTGGGCTCAGGGCGGTGCAGAAGTATTCCTCAAAAATATGACAAGGTTGTGGCGCAAAGAAAACTTTCGAATGTGG
CCGCTGTTTTTTGAATACTGCCTGACGACAATATGGGCCTTCACCTGCCTGGTCGGTTTCATTATTTACGCAGTCCAACT
TGCCGGTGTACCGTTAAATATTGAATTGACACATATCGCTGCGACACATACTGCCGGAATATTATTGTGTACGTTATGTT
TACTGCAATTTATTGTCAGCCTGATGATCGAGAATCGCTATGAGCATAATCTGACTTCATCGCTTTTCTGGATTATTTGG
TTCCCGGTTATTTTCTGGATGCTGAGCCTGGCAACGACATTGGTATCATTTACACGAGTCATGTTGATGCCTAAAAAGCA
ACGCGCCCGTTGGGTAAGTCCCGATCGCGGGATTCTGAGAGGTTAA

Upstream 100 bases:

>100_bases
GGAGTGAAAAACTATGGTTATTATCCCGACAATTTTCTGCATAACCAACCTGAAATAGACCTTATTCGTCCTGAGTTTTC
AACAGCCTGGTATCCGAAAA

Downstream 100 bases:

>100_bases
TATGAACAATTTAATTATTACGACCCGACAATCACCAGTACGTTTACTGGTTGATTATGTTGCCACAACCATCTTGTGGA
CATTATTTGCGTTGTTCATA

Product: N-glycosyltransferase PgaC

Products: NA

Alternate protein names: PGA synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm PGA synthesis protein PgaC; N-acetylglucosaminyltransferase PgaC [H]

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIMWIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISII
IPCFNEEKNVEETIHAALAQRYENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIKRTQRIYGNVFTVSGVIAAFRRS
ALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRALCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMW
PLFFEYCLTTIWAFTCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLTSSLFWIIW
FPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG

Sequences:

>Translated_441_residues
MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIMWIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISII
IPCFNEEKNVEETIHAALAQRYENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIKRTQRIYGNVFTVSGVIAAFRRS
ALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRALCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMW
PLFFEYCLTTIWAFTCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLTSSLFWIIW
FPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG
>Mature_441_residues
MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIMWIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISII
IPCFNEEKNVEETIHAALAQRYENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIKRTQRIYGNVFTVSGVIAAFRRS
ALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRALCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMW
PLFFEYCLTTIWAFTCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLTSSLFWIIW
FPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG

Specific function: Probable N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N- acetylglucosamine to produce the linear homopolymer poly-beta-1,6- N-acetyl-D-glucosamine (PGA), a biofilm adhesin polysaccharide [H]

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787259, Length=441, Percent_Identity=100, Blast_Score=910, Evalue=0.0,
Organism=Escherichia coli, GI1786560, Length=219, Percent_Identity=29.2237442922374, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 50766; Mature: 50766

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIMWIVGGVYFWVYRERHW
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCC
PWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRYENIEVIAVNDGSTDKTRA
CCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHH
ILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLVCIDGDALLDRDAAAYIVEPM
HHHHHHHHCCCEEEEEEECCCCCEEEEECCCCCCCCCEEEEECCCHHHCCCCHHHHCCCC
LYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIKRTQRIYGNVFTVSGVIAAFRRS
EECCCEEEEECCCCEEECCEEEEEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
ALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRALCWILMPETLKGLWKQRLRWAQGG
HHHHCCCCCCCCEECCCCEEEEEEEEEEEEEECCCEEEEEECHHHHHHHHHHHHHHHCCH
AEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAFTCLVGFIIYAVQLAGVPLNIELTHIA
HHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEE
ATHTAGILLCTLCLLQFIVSLMIENRYEHNLTSSLFWIIWFPVIFWMLSLATTLVSFTRV
HHHHHHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLMPKKQRARWVSPDRGILRG
HHCCCHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIMWIVGGVYFWVYRERHW
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCC
PWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRYENIEVIAVNDGSTDKTRA
CCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHH
ILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLVCIDGDALLDRDAAAYIVEPM
HHHHHHHHCCCEEEEEEECCCCCEEEEECCCCCCCCCEEEEECCCHHHCCCCHHHHCCCC
LYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIKRTQRIYGNVFTVSGVIAAFRRS
EECCCEEEEECCCCEEECCEEEEEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
ALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRALCWILMPETLKGLWKQRLRWAQGG
HHHHCCCCCCCCEECCCCEEEEEEEEEEEEEECCCEEEEEECHHHHHHHHHHHHHHHCCH
AEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAFTCLVGFIIYAVQLAGVPLNIELTHIA
HHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEE
ATHTAGILLCTLCLLQFIVSLMIENRYEHNLTSSLFWIIWFPVIFWMLSLATTLVSFTRV
HHHHHHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLMPKKQRARWVSPDRGILRG
HHCCCHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]