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The map label for this gene is rutA [H]

Identifier: 157160536

GI number: 157160536

Start: 1135161

End: 1136342

Strand: Reverse

Name: rutA [H]

Synonym: EcHS_A1127

Alternate gene names: 157160536

Gene position: 1136342-1135161 (Counterclockwise)

Preceding gene: 157160538

Following gene: 157160535

Centisome position: 24.47

GC content: 54.82

Gene sequence:

>1182_bases
TTGCAAACAAGCCATTACGCGGCTGAAAAGGATATGCAGGACGCAGTGCCCCGTCTGACTTTCACTTTACGAGACGAAGA
GAGGTTGATGATGAAAATTGGCGTATTCGTACCTATTGGCAACAACGGCTGGCTCATTTCGACCCACGCGCCGCAGTACA
TGCCGACCTTTGAACTGAATAAAGCCATCGTGCAAAAAGCGGAGCACTACCATTTCGATTTCGCCCTGTCGATGATCAAA
CTGCGTGGCTTTGGCGGCAAAACTGAGTTCTGGGATCACAACCTTGAGTCGTTCACCTTGATGGCGGGGCTGGCGGCCGT
GACCTCGCGCATTCAGATTTACGCCACCGCCGCCACCTTAACGTTACCTCCAGCAATCGTCGCCCGTATGGCCGCAACCA
TCGACTCAATCTCTGGCGGGCGTTTTGGCGTCAACCTCGTGACTGGCTGGCAAAAGCCCGAGTATGAGCAGATGGGTATC
TGGCCTGGCGATGACTATTTCTCCCGTCGTTACGACTATCTCACCGAGTATGTTCAGGTGCTGCGCGACCTGTGGGGCAC
GGGAAAAAGCGATTTTAAAGGCGATTTTTTCACCATGAATGATTGTCGCGTCAGTCCGCAACCGAGTGTCCCTATGAAAG
TGATCTGCGCCGGGCAAAGCGACGCTGGCATGGCGTTCTCCGCCCAGTATGCCGATTTCAACTTCTGTTTCGGCAAAGGC
GTAAATACACCCACGGCTTTCGCCCCGACCGCTGCGCGGATGAAACAGGCCGCAGAGCAAACCGGGCGCGACGTTGGCTC
TTATGTATTGTTTATGGTGATTGCCGATGAAACCGACGATGCCGCTCGCGCCAAATGGGAACACTACAAAGCGGGCGCGG
ATGAAGAGGCGTTAAGCTGGCTAACCGAACAAAGTCAGAAAGATACCCGCTCAGGTACTGACACCAACGTCCGTCAGATG
GCCGATCCCACTTCGGCGGTAAACATCAATATGGGGACGTTAGTCGGTTCTTACGCCAGTGTCGCGCGCATGTTAGATGA
AGTCGCAAGCGTGCCTGGTGCCGAAGGCGTGCTGTTAACCTTCGACGATTTTCTGTCGGGAATCGAAACCTTCGGCGAGC
GCATTCAACCACTGATGCAGTGCCGCGCCCATCTCCCTGTGCTGACTCAGGAGGTGGCATGA

Upstream 100 bases:

>100_bases
CAACTGTTTTGACCGTTTAGTCCGCTTTTTACCAGATATTTTAGATAACTTAACATTAACACATTAATAACATTCACTTT
TTTAAAAACTGGCATCCACT

Downstream 100 bases:

>100_bases
TGACGACATTAACCGCTCGACCGGAAGCCATTACCTTCGATCCGCAGCAAAGTGCGCTGATCGTGGTGGATATGCAAAAC
GCCTATGCCACGCCAGGCGG

Product: putative monooxygenase rutA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MQTSHYAAEKDMQDAVPRLTFTLRDEERLMMKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDFALSMIK
LRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAATIDSISGGRFGVNLVTGWQKPEYEQMGI
WPGDDYFSRRYDYLTEYVQVLRDLWGTGKSDFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKG
VNTPTAFAPTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQKDTRSGTDTNVRQM
ADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPVLTQEVA

Sequences:

>Translated_393_residues
MQTSHYAAEKDMQDAVPRLTFTLRDEERLMMKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDFALSMIK
LRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAATIDSISGGRFGVNLVTGWQKPEYEQMGI
WPGDDYFSRRYDYLTEYVQVLRDLWGTGKSDFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKG
VNTPTAFAPTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQKDTRSGTDTNVRQM
ADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPVLTQEVA
>Mature_393_residues
MQTSHYAAEKDMQDAVPRLTFTLRDEERLMMKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDFALSMIK
LRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAATIDSISGGRFGVNLVTGWQKPEYEQMGI
WPGDDYFSRRYDYLTEYVQVLRDLWGTGKSDFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKG
VNTPTAFAPTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQKDTRSGTDTNVRQM
ADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPVLTQEVA

Specific function: Catalyzes the pyrimidine ring opening between N-3 and C- 4 by an unusual flavin hydroperoxide-catalyzed mechanism to yield ureidoacrylate peracid. It cleaves pyrmidine rings directly by adding oxygen atoms, making a toxic ureidoacrylate peracid product wh

COG id: COG2141

COG function: function code C; Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ntaA/snaA/soxA(dszA) monooxygenase family. RutA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787247, Length=382, Percent_Identity=99.2146596858639, Blast_Score=794, Evalue=0.0,
Organism=Escherichia coli, GI1787166, Length=305, Percent_Identity=24.5901639344262, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011251
- InterPro:   IPR019914
- InterPro:   IPR016048 [H]

Pfam domain/function: PF00296 Bac_luciferase [H]

EC number: NA

Molecular weight: Translated: 43510; Mature: 43510

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTSHYAAEKDMQDAVPRLTFTLRDEERLMMKIGVFVPIGNNGWLISTHAPQYMPTFELN
CCCCCHHHHHHHHHCCCCEEEEECCHHHEEEEEEEEEEECCCCEEEECCCCCCCCCHHHH
KAIVQKAEHYHFDFALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEEEEEE
TLPPAIVARMAATIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQV
ECCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHH
LRDLWGTGKSDFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKG
HHHHHCCCCCCCCCCCEEECCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCCEEEECCC
VNTPTAFAPTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSW
CCCCCCCCCHHHHHHHHHHHHCCHHHCEEEEEEEECCCCCHHHHHHHHHCCCCCHHHHHH
LTEQSQKDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLT
HHHHHHHHCCCCCCCCHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
FDDFLSGIETFGERIQPLMQCRAHLPVLTQEVA
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCC
>Mature Secondary Structure
MQTSHYAAEKDMQDAVPRLTFTLRDEERLMMKIGVFVPIGNNGWLISTHAPQYMPTFELN
CCCCCHHHHHHHHHCCCCEEEEECCHHHEEEEEEEEEEECCCCEEEECCCCCCCCCHHHH
KAIVQKAEHYHFDFALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEEEEEE
TLPPAIVARMAATIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQV
ECCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHH
LRDLWGTGKSDFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKG
HHHHHCCCCCCCCCCCEEECCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCCEEEECCC
VNTPTAFAPTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSW
CCCCCCCCCHHHHHHHHHHHHCCHHHCEEEEEEEECCCCCHHHHHHHHHCCCCCHHHHHH
LTEQSQKDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLT
HHHHHHHHCCCCCCCCHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
FDDFLSGIETFGERIQPLMQCRAHLPVLTQEVA
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA