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The map label for this gene is ycbF [H]

Identifier: 157160465

GI number: 157160465

Start: 1065535

End: 1066272

Strand: Direct

Name: ycbF [H]

Synonym: EcHS_A1053

Alternate gene names: 157160465

Gene position: 1065535-1066272 (Clockwise)

Preceding gene: 157160464

Following gene: 157160466

Centisome position: 22.95

GC content: 51.49

Gene sequence:

>738_bases
ATGCCAGCGCGACATTTGTACTTCATTATGACTAATACGTGGAACAGATTGGCGCTCTTGATTTTCGCCGTTTTATCGCT
GCTGGTGGCGGGTGAATTGCAGGCAGGAGTGGTGGTCGGCGGAACGCGATTTATCTTTCCGGCAGACAGAGAATCGATAT
CTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGG
GAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCG
CTTGCTGAGAACGGAAAGCGACATCTTGCCTGTGGATCGCGAAACGCTATTTGAGTTAAGCATTGCCAGCGTGCCATCCG
GCAAAGTTGAAAATCAGAGCGTAAAAGTGGCGATGCGCTCGGTATTTAAACTGTTCTGGCGACCCGAAGGTTTGCCGGGC
GACCCGCTGGAAGCTTATCAACAATTACGCTGGACACGGAATTCGCAGGGTGTACAACTCACTAACCCAACGCCTTATTA
CATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGA
CAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAG
GAGCAAAATCTGCCCTGA

Upstream 100 bases:

>100_bases
CAGGCGAGTAAAGACTACAGCCTACATAGCGGTAAGGTGCCATTAACTTTTTATGCGCAATTGCGACCTGTCAATAGCGA
TGTGCAGTCGGGTAAAGTGA

Downstream 100 bases:

>100_bases
AACAGGTTCGGAAAACGTTTGCGTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCG
GTTTGCACACCGGGAATCCA

Product: putativi pili assembly chaperone

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRFIFPADRESISILLTNTSQESWLINSKINRPTRWAGG
EASTVPAPLLAAPPLILLKPGTTGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNVGVVPPKSQRQTSWCQAIAPCHVAWRAINDYGGLSAKK
EQNLP

Sequences:

>Translated_245_residues
MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRFIFPADRESISILLTNTSQESWLINSKINRPTRWAGG
EASTVPAPLLAAPPLILLKPGTTGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNVGVVPPKSQRQTSWCQAIAPCHVAWRAINDYGGLSAKK
EQNLP
>Mature_244_residues
PARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRFIFPADRESISILLTNTSQESWLINSKINRPTRWAGGE
ASTVPAPLLAAPPLILLKPGTTGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPGD
PLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNVGVVPPKSQRQTSWCQAIAPCHVAWRAINDYGGLSAKKE
QNLP

Specific function: Could be required for the biogenesis of a putative fimbria [H]

COG id: COG3121

COG function: function code NU; P pilus assembly protein, chaperone PapD

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI87081806, Length=236, Percent_Identity=100, Blast_Score=476, Evalue=1e-136,
Organism=Escherichia coli, GI1790771, Length=223, Percent_Identity=32.2869955156951, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1787171, Length=222, Percent_Identity=33.7837837837838, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1786333, Length=242, Percent_Identity=30.5785123966942, Blast_Score=104, Evalue=4e-24,
Organism=Escherichia coli, GI1789532, Length=228, Percent_Identity=28.0701754385965, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1786743, Length=236, Percent_Identity=27.5423728813559, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI87082203, Length=216, Percent_Identity=25.9259259259259, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786936, Length=217, Percent_Identity=29.0322580645161, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1788428, Length=233, Percent_Identity=24.8927038626609, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1788677, Length=176, Percent_Identity=27.2727272727273, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 26983; Mature: 26851

Theoretical pI: Translated: 10.26; Mature: 10.26

Prosite motif: PS00635 PILI_CHAPERONE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRFIFPADRESISILLTNT
CCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEECCEEEEEECCCCEEEEEEECC
SQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGTTGTLRLLRTESDILPVDR
CCCCEEEECCCCCCCCCCCCCCCCCCCCHHHCCCEEEECCCCCCEEEEEECCCCCCCCCH
ETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPGDPLEAYQQLRWTRNSQGVQL
HHHHEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEE
TNPTPYYINLIQVSVNGKALSNVGVVPPKSQRQTSWCQAIAPCHVAWRAINDYGGLSAKK
CCCCCEEEEEEEEEECCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
EQNLP
CCCCC
>Mature Secondary Structure 
PARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRFIFPADRESISILLTNT
CCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEECCEEEEEECCCCEEEEEEECC
SQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGTTGTLRLLRTESDILPVDR
CCCCEEEECCCCCCCCCCCCCCCCCCCCHHHCCCEEEECCCCCCEEEEEECCCCCCCCCH
ETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPGDPLEAYQQLRWTRNSQGVQL
HHHHEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEE
TNPTPYYINLIQVSVNGKALSNVGVVPPKSQRQTSWCQAIAPCHVAWRAINDYGGLSAKK
CCCCCEEEEEEEEEECCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
EQNLP
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 2992959; 7984428 [H]