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The map label for this gene is lipB [H]

Identifier: 157160126

GI number: 157160126

Start: 697720

End: 698361

Strand: Reverse

Name: lipB [H]

Synonym: EcHS_A0682

Alternate gene names: 157160126

Gene position: 698361-697720 (Counterclockwise)

Preceding gene: 157160127

Following gene: 157160125

Centisome position: 15.04

GC content: 47.66

Gene sequence:

>642_bases
TTGTATCAGGATAAAATTCTTGTCCGCCAGCTCGGTCTTCAGCCTTACGAGCCAATCTCCCAGGCTATGCATGAATTCAC
CGATACCCGCGATGATAGTACCCTTGATGAAATCTGGCTGGTCGAGCACTATCCGGTATTCACCCAAGGTCAGGCAGGAA
AAGCGGAGCACATTTTAATGCCGGGTGATATTCCGGTGATCCAGAGCGATCGCGGTGGGCAGGTGACTTATCACGGGCCG
GGGCAACAGGTGATGTATGTGTTGCTTAACCTGAAACGCCGTAAACTCGGTGTGCGTGAACTGGTGACCTTGCTTGAGCA
AACAGTGGTGAATACCCTGGCTGAACTGGGTATAGAAGCGCATCCTCGGGCTGACGCGCCAGGTGTCTATGTTGGGGAAA
AGAAAATTTGCTCACTGGGTTTACGTATTCGACGCGGTTGTTCATTCCACGGTCTGGCATTAAACGTCAATATGGATCTT
TCACCATTTTTACGTATTAATCCTTGTGGGTATGCCGGAATGGAAATGGCTAAAATATCACAATGGAAACCCGAAGCGAC
GACTAATAATATTGCTCCACGTTTACTGGAAAATATTTTAGCGCTACTAAACAATCCGGACTTCGAATATATTACCGCTT
AA

Upstream 100 bases:

>100_bases
CTCGCTTCTCCGTTACCCGGCTCCTGGTCGGGTAACTCCCCTCTCCTCGCTGTGATATACTTTTCCCCCACTTTTACTCA
TTCTCCACGGAGATGCCGTT

Downstream 100 bases:

>100_bases
TTCCATACATCAATGGCCCAATTTACACTGGGTCATTACTCATAATACCTGCTAATGCTTTACATCTCCTTCGCGTTTGT
ATATTTTCTTTATCACTTGC

Product: lipoyltransferase

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHYPVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGP
GQQVMYVLLNLKRRKLGVRELVTLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA

Sequences:

>Translated_213_residues
MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHYPVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGP
GQQVMYVLLNLKRRKLGVRELVTLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA
>Mature_213_residues
MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHYPVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGP
GQQVMYVLLNLKRRKLGVRELVTLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Homo sapiens, GI221554520, Length=116, Percent_Identity=40.5172413793103, Blast_Score=68, Evalue=7e-12,
Organism=Escherichia coli, GI87081767, Length=213, Percent_Identity=100, Blast_Score=443, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6323268, Length=152, Percent_Identity=35.5263157894737, Blast_Score=92, Evalue=6e-20,
Organism=Drosophila melanogaster, GI21355483, Length=147, Percent_Identity=35.3741496598639, Blast_Score=90, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 23883; Mature: 23883

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHYPVFTQGQAGKAEHILM
CCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHEEEEECCCEEECCCCCCCCEEEE
PGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELVTLLEQTVVNTLAELGIEA
CCCCCEEECCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCC
HPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDLSPFLRINPCGYAGMEMAKIS
CCCCCCCCEEECCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEECCCCCCCCHHHHHH
QWKPEATTNNIAPRLLENILALLNNPDFEYITA
CCCCCCCCCCCCHHHHHHHHHHHCCCCCEEECC
>Mature Secondary Structure
MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHYPVFTQGQAGKAEHILM
CCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHEEEEECCCEEECCCCCCCCEEEE
PGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELVTLLEQTVVNTLAELGIEA
CCCCCEEECCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCC
HPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDLSPFLRINPCGYAGMEMAKIS
CCCCCCCCEEECCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEECCCCCCCCHHHHHH
QWKPEATTNNIAPRLLENILALLNNPDFEYITA
CCCCCCCCCCCCHHHHHHHHHHHCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA