Click here to switch to the map view.

The map label for this gene is ybeF

Identifier: 157160125

GI number: 157160125

Start: 696508

End: 697461

Strand: Reverse

Name: ybeF

Synonym: EcHS_A0681

Alternate gene names: 157160125

Gene position: 697461-696508 (Counterclockwise)

Preceding gene: 157160126

Following gene: 157160124

Centisome position: 15.02

GC content: 44.97

Gene sequence:

>954_bases
GTGGATAGTAATAATCAAATTGAACCCTGTTTAAGCAGAAAGTCGTCAGAAGGTAAACCACAAATATTTACAACCTTACG
AAATATTGATCTTAACCTTCTGACTATTTTTGAAGCTGTATATGTACATAAAGGGATCGTTAATGCAGCGAAAGTGCTTA
ATCTGACCCCCTCGGCAATCAGTCAGTCTATTCAGAAACTGCGCGTTATATTCCCTGACCCATTGTTTATTCGCAAAGGC
CAGGGTGTCACTCCTACCGCATTTGCGATGCATCTACATGAGTATATCAGTCAGGGCCTTGAGTCCATTCTTGGCGCGCT
GGATATCGAAGGAAGCTATGATAAGCAACGAACGATAACTATTGCGACCACTCCCTCGGTCGGAGCCCTGGTCCTTCCTG
TCATCTATCGGGCGATTAAAACTCACTATCCGCAGCTTTTACTGCGCAACCCGCCCATCAGCGACGCGGAAAACCAACTC
AGTCAGTTTCAAACCGATCTCATCATCGATAACATGTTTTGCACCAATCGTACGGTGCAACATCATGTTCTGTTCACCGA
CAATATGGTGTTAATTTGCCGCGAGGGAAATCCACTACTCTCTTTAGAAGATGACAGAGAGACTATCGACAACGCTGCGC
ATGTACTTCTGTTACCGGAAGAGCAAAATTTCAGCGGTCTGCGGCAGAGAGTTCAAGAAATGTTTCCGGACCGGCAAATC
AATTTCACCAGCTACAACATTTTGACAATCGCTGCACTGGTTGCCAACAGTGACATGTTAGCGATTATTCCCAGCCGTTT
TTATAACCTGTTTAGCCGCTGCTGGCCGCTGGAAAAACTGCCTTTTCCGTCCTTAAATGAGGAGCAAATAGATTTCTCTA
TCCACTACAACAAATTCAGCCTGCGTGATCCCATCCTGCACGGAGTAATAGATGTTATCCGTAATGCATTTTGA

Upstream 100 bases:

>100_bases
AGTTGATTATGGTGAGCAAAAGCGTTCCGTTCCAATTAGACATAAGATTATTGTAATTTTTTAAATATTATTTTTCCATG
AATAAAATTGGAGTAAGTGC

Downstream 100 bases:

>100_bases
AAAAGTATTGTTACAGCCAGATCACAGCGCAGAACGATAAAATGGCACAATCCAACAACAATTTTACATTTTCGCGACCG
CTTTGGCTGCTTTCAGGTCC

Product: putative DNA-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHKGIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKG
QGVTPTAFAMHLHEYISQGLESILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLLPEEQNFSGLRQRVQEMFPDRQI
NFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF

Sequences:

>Translated_317_residues
MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHKGIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKG
QGVTPTAFAMHLHEYISQGLESILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLLPEEQNFSGLRQRVQEMFPDRQI
NFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF
>Mature_317_residues
MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHKGIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKG
QGVTPTAFAMHLHEYISQGLESILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLLPEEQNFSGLRQRVQEMFPDRQI
NFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI87081766, Length=317, Percent_Identity=100, Blast_Score=651, Evalue=0.0,
Organism=Escherichia coli, GI1786820, Length=299, Percent_Identity=30.1003344481605, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI87081684, Length=272, Percent_Identity=26.4705882352941, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790147, Length=296, Percent_Identity=23.3108108108108, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): YBEF_ECOLI (P30979)

Other databases:

- EMBL:   L07636
- EMBL:   U82598
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   C64797
- RefSeq:   AP_001278.1
- RefSeq:   NP_415162.4
- ProteinModelPortal:   P30979
- SMR:   P30979
- STRING:   P30979
- EnsemblBacteria:   EBESCT00000002496
- EnsemblBacteria:   EBESCT00000018040
- GeneID:   945219
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW0624
- KEGG:   eco:b0629
- EchoBASE:   EB1551
- EcoGene:   EG11594
- eggNOG:   COG0583
- GeneTree:   EBGT00050000008343
- HOGENOM:   HBG416490
- OMA:   PTAYATH
- ProtClustDB:   PRK11482
- BioCyc:   EcoCyc:EG11594-MONOMER
- Genevestigator:   P30979
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 35961; Mature: 35961

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHKGIVNAAKVLNLTPSAI
CCCCCCCCHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
SQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLESILGALDIEGSYDKQRTIT
HHHHHHHEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEE
IATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQLSQFQTDLIIDNMFCTNRTVQ
EEECCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHCHHHCCCCEE
HHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLLPEEQNFSGLRQRVQEMFPDRQI
EEEEEECCEEEEEECCCCEEEECCHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCEE
NFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKLPFPSLNEEQIDFSIHYNKFS
CEEEHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECEEC
LRDPILHGVIDVIRNAF
CCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHKGIVNAAKVLNLTPSAI
CCCCCCCCHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
SQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLESILGALDIEGSYDKQRTIT
HHHHHHHEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEE
IATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQLSQFQTDLIIDNMFCTNRTVQ
EEECCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHCHHHCCCCEE
HHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLLPEEQNFSGLRQRVQEMFPDRQI
EEEEEECCEEEEEECCCCEEEECCHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCEE
NFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKLPFPSLNEEQIDFSIHYNKFS
CEEEHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECEEC
LRDPILHGVIDVIRNAF
CCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8444795; 8905232; 9278503