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The map label for this gene is fepB

Identifier: 157160088

GI number: 157160088

Start: 659635

End: 660591

Strand: Reverse

Name: fepB

Synonym: EcHS_A0643

Alternate gene names: 157160088

Gene position: 660591-659635 (Counterclockwise)

Preceding gene: 157160096

Following gene: 157160086

Centisome position: 14.23

GC content: 54.96

Gene sequence:

>957_bases
GTGAGACTCGCCCCGCTCTACCGCAACGCCCTTCTATTAACAGGACTTTTGCTTTCAGGAATAGCCGCAGTTCAGGCCGC
TGACTGGCCGCGTCAGATTACTGACAGCCGTGGCACACATACACTGGAAAGCCAGCCGCAGCGTATTGTTTCCACCAGCG
TCACCCTGACCGGCTCACTGCTGGCGATTGATGCACCAGTGATCGCCAGCGGCGCGACCACGCCAAATAACCGCGTCGCG
GATGACCAGGGCTTTTTACGCCAGTGGAGCAAGGTGGCGAAAGAACGCAAACTGCAACGGCTCTATATCGGCGAACCGAG
CGCCGAAGCCGTTGCCGCGCAAATGCCGGATCTGATTTTAATTAGCGCAACTGGCGGGGATTCGGCGCTGGCGCTGTATG
ATCAGCTTTCCACCATCGCCCCGACATTAATCATCAATTACGACGACAAAAGCTGGCAGTCGCTGTTAACGCAGCTTGGC
GAAATTACCGGGCATGAGAAACAAGCGGCAGAGCGGATTGCGCAGTTTGATAAGCAACTGGCGGCGGCGAAAGAGCAAAT
CAAATTACCGCCGCAGCCGGTCACTGCCATTGTCTATACTGCCGCTGCACACAGTGCCAATCTCTGGACGCCAGAATCAG
CACAAGGGCAGATGCTGGAACAACTCGGCTTTACGCTGGCGAAGTTACCCGCAGGGTTAAATGCCAGCCAAAGTCAGGGT
AAACGCCATGACATCATTCAGCTTGGTGGGGAAAATCTGGCTGCAGGGTTAAATGGCGAGTCACTATTCCTGTTCGCCGG
TGATCAGAAAGATGCCGATGCTATTTATGCTAATCCGCTGCTCGCGCACCTGCCTGCAGTACAAAACAAGCAGGTTTATG
CGCTGGGAACCGAGACGTTCCGTCTGGATTACTACAGCGCCATGCAAGTGCTGGATAGGCTTAAGGCGCTGTTTTAA

Upstream 100 bases:

>100_bases
TCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACGGATTGTGCGCTTTGTCGAATTTGTCATTACGCCCTTAACCTTAT
TAATAACAGGAAGCTGATTT

Downstream 100 bases:

>100_bases
GCATTAACTGTCGGACGCTGTCACCTGCGGCGGCGTCTGGCGAAAATGTCGCAACTCCACCAGCACCAGCAATAACAACA
CGCCGATAATCAACAAACCA

Product: iron-enterobactin transporter periplasmic binding protein

Products: ADP; phosphate; ferric enterobactin [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLESQPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVA
DDQGFLRQWSKVAKERKLQRLYIGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQMLEQLGFTLAKLPAGLNASQSQG
KRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIYANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLDRLKALF

Sequences:

>Translated_318_residues
MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLESQPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVA
DDQGFLRQWSKVAKERKLQRLYIGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQMLEQLGFTLAKLPAGLNASQSQG
KRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIYANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLDRLKALF
>Mature_318_residues
MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLESQPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVA
DDQGFLRQWSKVAKERKLQRLYIGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQMLEQLGFTLAKLPAGLNASQSQG
KRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIYANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLDRLKALF

Specific function: Binds ferrienterobactin; part of the binding-protein- dependent transport system for uptake of ferrienterobactin

COG id: COG4592

COG function: function code P; ABC-type Fe2+-enterobactin transport system, periplasmic component

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain

Homologues:

Organism=Escherichia coli, GI1786807, Length=318, Percent_Identity=100, Blast_Score=639, Evalue=0.0,
Organism=Escherichia coli, GI87082411, Length=294, Percent_Identity=24.4897959183673, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FEPB_ECOL6 (P0AEL7)

Other databases:

- EMBL:   AE014075
- RefSeq:   NP_752610.1
- ProteinModelPortal:   P0AEL7
- EnsemblBacteria:   EBESCT00000043315
- GeneID:   1035309
- GenomeReviews:   AE014075_GR
- KEGG:   ecc:c0679
- GeneTree:   EBGT00050000010019
- HOGENOM:   HBG649679
- OMA:   SFRIDYY
- ProtClustDB:   PRK10957
- InterPro:   IPR002491

Pfam domain/function: PF01497 Peripla_BP_2

EC number: NA

Molecular weight: Translated: 34283; Mature: 34283

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLESQPQRIVSTSVTLTGSL
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHCCCHHHHHHHHEEECCE
LAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLYIGEPSAEAVAAQMPDLIL
EEECCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEE
ISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLGEITGHEKQAAERIAQFDKQL
EEECCCCCHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
AAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQMLEQLGFTLAKLPAGLNASQSQG
HHHHHHCCCCCCCCEEEEEEHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHCC
KRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIYANPLLAHLPAVQNKQVYALGTETF
CCCCCEEECCCCEEECCCCCEEEEEECCCCCCCHHHHCHHHHHCCCCCCCEEEEECCCEE
RLDYYSAMQVLDRLKALF
EHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLESQPQRIVSTSVTLTGSL
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHCCCHHHHHHHHEEECCE
LAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLYIGEPSAEAVAAQMPDLIL
EEECCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEE
ISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLGEITGHEKQAAERIAQFDKQL
EEECCCCCHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
AAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQMLEQLGFTLAKLPAGLNASQSQG
HHHHHHCCCCCCCCEEEEEEHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHCC
KRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIYANPLLAHLPAVQNKQVYALGTETF
CCCCCEEECCCCEEECCCCCEEEEEECCCCCCCHHHHCHHHHHCCCCCCCEEEEECCCEE
RLDYYSAMQVLDRLKALF
EHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ferric enterobactin [Periplasm]; H2O [C]

Specific reaction: ATP + ferric enterobactin [Periplasm] + H2O = ADP + phosphate + ferric enterobactin [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157