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The map label for this gene is yahI [H]

Identifier: 157159836

GI number: 157159836

Start: 401049

End: 401999

Strand: Direct

Name: yahI [H]

Synonym: EcHS_A0383

Alternate gene names: 157159836

Gene position: 401049-401999 (Clockwise)

Preceding gene: 157159835

Following gene: 157159837

Centisome position: 8.64

GC content: 56.05

Gene sequence:

>951_bases
ATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGC
GGAGGCGGTGAAAGCCGTGGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGTAACG
GGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACGAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCGAAC
TGTGTGGCAGATACGCAGGGCGGCATCGGCTATCTGATCCAACAGGCGCTGAATAACCGGCTGGCGCGTCACGGCGAGAA
AAAAGCCGTCACCGTGATAACTCAGGTGGAAGTGGATAAAAACGATCCAGGTTTTGCCCATCCCACCAAGCCCATTGGTG
CATTCTTCAGTGAAAGCCAGCGTGACAAACTACAAAAGGCAAACCCTGACTGGTGTTTTGTTGAAGATGCCGGACGGGGC
TATCGCCGCGTGGTCGCCTCGCCGGAACCGAAACGTATTGTCGAAGCACCCGCCATTAAGGCGCTGATCCAAAAAGGTTT
TGTCGTAATTGGCGCGGGCGGCGGTGGAATTCCGGTAGTGCGTACTGAAGCGGGGGATTACCAAAGCGTGGACGCGGTTA
TTGACAAAGATCTCTCTACCGCGCTGCTGGCCCGTGAAATTCACGCCGACATTCTGGTGATCACCACCGGCGTCGAAAAA
GTCTGTATCCACTTTGGTAAACCGCAGCAGCAGGCGCTGGATCGGGTGGATATTGCCACCATGACTCGCTATATGCAGGA
AGGGCATTTTCCGCCCGGCAGCATGTTGCCAAAAATCATCGCCAGCCTGACGTTCCTGGAGCAGGGTGGCAAAGAAGTGA
TTATCACCACGCCGGAATGCCTGCCTGCGGCGCTGCGCGGTGAAACGGGCACCCATATTATTAAAACGTAA

Upstream 100 bases:

>100_bases
CCTACGGATGGCCCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCTAATCACCC
TCTGTAGCGGACATAACAAC

Downstream 100 bases:

>100_bases
GGACGTAGGATGAAAGAAAGCAATAGCCGCCGTGAATTTCTGAGCCAGAGCGGTAAGATGGTCACCGCCGCCGCGCTGTT
TGGTACCTCTGTGCCGCTCG

Product: putative carbamate kinase

Products: ADP; carbamoyl phosphate

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASDYDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLAN
CVADTQGGIGYLIQQALNNRLARHGEKKAVTVITQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
YRRVVASPEPKRIVEAPAIKALIQKGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDLSTALLAREIHADILVITTGVEK
VCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSMLPKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT

Sequences:

>Translated_316_residues
MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASDYDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLAN
CVADTQGGIGYLIQQALNNRLARHGEKKAVTVITQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
YRRVVASPEPKRIVEAPAIKALIQKGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDLSTALLAREIHADILVITTGVEK
VCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSMLPKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT
>Mature_316_residues
MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASDYDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLAN
CVADTQGGIGYLIQQALNNRLARHGEKKAVTVITQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
YRRVVASPEPKRIVEAPAIKALIQKGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDLSTALLAREIHADILVITTGVEK
VCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSMLPKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT

Specific function: Unknown

COG id: COG0549

COG function: function code E; Carbamate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbamate kinase family [H]

Homologues:

Organism=Escherichia coli, GI1786516, Length=316, Percent_Identity=98.1012658227848, Blast_Score=637, Evalue=0.0,
Organism=Escherichia coli, GI1789238, Length=316, Percent_Identity=45.8860759493671, Blast_Score=259, Evalue=1e-70,
Organism=Escherichia coli, GI1786732, Length=313, Percent_Identity=38.3386581469649, Blast_Score=175, Evalue=4e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR003964 [H]

Pfam domain/function: PF00696 AA_kinase [H]

EC number: 2.7.2.2

Molecular weight: Translated: 33973; Mature: 33973

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASDYDIVLTHGNGPQVGLD
CCCEEEEEECCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHH
LRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLARHGEKKAVTVITQVEVDK
HHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECC
NDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRGYRRVVASPEPKRIVEAPAIK
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEECCCCCCHHHHHHHHHH
ALIQKGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDLSTALLAREIHADILVITTGVEK
HHHHCCCEEEECCCCCCCEEEECCCCCHHHHHHHHCCHHHHHHHHHHCCCEEEEECCHHH
VCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSMLPKIIASLTFLEQGGKEVIITTPEC
HHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCHH
LPAALRGETGTHIIKT
HHHHHCCCCCCEEECC
>Mature Secondary Structure
MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASDYDIVLTHGNGPQVGLD
CCCEEEEEECCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHH
LRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLARHGEKKAVTVITQVEVDK
HHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECC
NDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRGYRRVVASPEPKRIVEAPAIK
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEECCCCCCHHHHHHHHHH
ALIQKGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDLSTALLAREIHADILVITTGVEK
HHHHCCCEEEECCCCCCCEEEECCCCCHHHHHHHHCCHHHHHHHHHHCCCEEEEECCHHH
VCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSMLPKIIASLTFLEQGGKEVIITTPEC
HHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCHH
LPAALRGETGTHIIKT
HHHHHCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; NH3; CO2

Specific reaction: ATP + NH3 + CO2 = ADP + carbamoyl phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]