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The map label for this gene is thiD

Identifier: 156980409

GI number: 156980409

Start: 2226529

End: 2227359

Strand: Reverse

Name: thiD

Synonym: SAHV_2078

Alternate gene names: 156980409

Gene position: 2227359-2226529 (Counterclockwise)

Preceding gene: 156980410

Following gene: 156980408

Centisome position: 77.33

GC content: 35.5

Gene sequence:

>831_bases
ATGATTAAACCTAAAATAGCATTAACCATTGCAGGTACTGATCCAACAGGTGGTGCCGGCGTAATGGCTGATTTAAAATC
ATTTCATTCATGTGGTGTATATGGTATGGGCGTCGTTACAAGTATTGTTGCTCAAAATACATTGGGCGTACAACATATTC
ATAATTTAAATCATCAATGGGTAGATGAACAACTTGATAGTGTCTTCAATGATACCTTACCTCATGCTATTAAAACGGGG
ATGATTGCTACAGCAGATACTATGGAAACGATTCGTCATTATTTAATGCAACATGAATCTATTCCATATGTAATTGATCC
TGTTATGTTGGCGAAAAGCGGTGATTCATTAATGGATAATGACACAAAGCAAAACTTGCAGCATACGTTATTGCCATTAG
CTGACGTAGTAACACCGAATTTACCAGAAGCTGAAGAAATAACGGGACTAACCATTGATAGTGAAGAAAAAATTATGCAG
GCTGGCCGCATCTTTATTAATGAGATTGGTAGTAAAGGTATCATCATTAAAGGCGGTCATTCAAATGATACTGATATAGC
AAAAGATTATTTATTTACTAACGAAGGTGTTCAAACATTTGAAAATGAACGATTTAAAACAAAACATACGCATGGAACAG
GGTGTACATTTTCAGCAGTTATAACGGCAGAACTTGCAAAAGGTAGACCATTATTTGAGGCTGTACACAAGGCTAAAAAG
TTTATTTCAATGAGTATACAATATACACCTGAAATCGGCCGTGGTAGAGGACCAGTGAATCATTTTGCATATTTAAAGAA
AGAGGGATTAGACGATGAATTATCTAAATAA

Upstream 100 bases:

>100_bases
GAACAAGTAAAGCAAGTATTTTTAGAAAGTTGCATACATGAGCGTCGATTTTTCAATATGGCTATGACACTAGAACAATG
GGAATTTGGAGGAAAAGTAA

Downstream 100 bases:

>100_bases
AATACGTATTGAAAACCCATTAACAATTTGTTATACAAACGATGTAGTTAAAAATTTTACAGCGAATGGTTTATTAAGTA
TTGGTGCGAGCCCTGCCATG

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MIKPKIALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVAQNTLGVQHIHNLNHQWVDEQLDSVFNDTLPHAIKTG
MIATADTMETIRHYLMQHESIPYVIDPVMLAKSGDSLMDNDTKQNLQHTLLPLADVVTPNLPEAEEITGLTIDSEEKIMQ
AGRIFINEIGSKGIIIKGGHSNDTDIAKDYLFTNEGVQTFENERFKTKHTHGTGCTFSAVITAELAKGRPLFEAVHKAKK
FISMSIQYTPEIGRGRGPVNHFAYLKKEGLDDELSK

Sequences:

>Translated_276_residues
MIKPKIALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVAQNTLGVQHIHNLNHQWVDEQLDSVFNDTLPHAIKTG
MIATADTMETIRHYLMQHESIPYVIDPVMLAKSGDSLMDNDTKQNLQHTLLPLADVVTPNLPEAEEITGLTIDSEEKIMQ
AGRIFINEIGSKGIIIKGGHSNDTDIAKDYLFTNEGVQTFENERFKTKHTHGTGCTFSAVITAELAKGRPLFEAVHKAKK
FISMSIQYTPEIGRGRGPVNHFAYLKKEGLDDELSK
>Mature_276_residues
MIKPKIALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVAQNTLGVQHIHNLNHQWVDEQLDSVFNDTLPHAIKTG
MIATADTMETIRHYLMQHESIPYVIDPVMLAKSGDSLMDNDTKQNLQHTLLPLADVVTPNLPEAEEITGLTIDSEEKIMQ
AGRIFINEIGSKGIIIKGGHSNDTDIAKDYLFTNEGVQTFENERFKTKHTHGTGCTFSAVITAELAKGRPLFEAVHKAKK
FISMSIQYTPEIGRGRGPVNHFAYLKKEGLDDELSK

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family

Homologues:

Organism=Escherichia coli, GI1788420, Length=259, Percent_Identity=42.8571428571429, Blast_Score=208, Evalue=2e-55,
Organism=Escherichia coli, GI1788758, Length=181, Percent_Identity=24.3093922651934, Blast_Score=61, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6325378, Length=269, Percent_Identity=30.8550185873606, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6324997, Length=270, Percent_Identity=34.8148148148148, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6324517, Length=268, Percent_Identity=33.2089552238806, Blast_Score=116, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THID_STAAM (P66915)

Other databases:

- EMBL:   BA000017
- RefSeq:   NP_372617.1
- ProteinModelPortal:   P66915
- SMR:   P66915
- STRING:   P66915
- World-2DPAGE:   0002:P66915
- EnsemblBacteria:   EBSTAT00000008171
- GeneID:   1122110
- GenomeReviews:   BA000017_GR
- KEGG:   sav:SAV2093
- eggNOG:   COG0351
- GeneTree:   EBGT00050000024460
- HOGENOM:   HBG476673
- OMA:   FSAMRAY
- ProtClustDB:   PRK06427
- BioCyc:   SAUR158878:SAV2093-MONOMER
- InterPro:   IPR013749
- InterPro:   IPR004399
- TIGRFAMs:   TIGR00097

Pfam domain/function: PF08543 Phos_pyr_kin

EC number: =2.7.1.49; =2.7.4.7

Molecular weight: Translated: 30243; Mature: 30243

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: BINDING 45-45

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKPKIALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVAQNTLGVQHIHNLNHQW
CCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHCCHHH
VDEQLDSVFNDTLPHAIKTGMIATADTMETIRHYLMQHESIPYVIDPVMLAKSGDSLMDN
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEECHHHEECCCCCHHCC
DTKQNLQHTLLPLADVVTPNLPEAEEITGLTIDSEEKIMQAGRIFINEIGSKGIIIKGGH
CHHHHHHHHHHHHHHHHCCCCCCHHHHCCCEECCHHHHHHHHHHHHHHCCCCCEEEECCC
SNDTDIAKDYLFTNEGVQTFENERFKTKHTHGTGCTFSAVITAELAKGRPLFEAVHKAKK
CCCHHHHHHHHHCCCCHHHHHCCCCEECCCCCCCCEEHHHHHHHHHCCCHHHHHHHHHHH
FISMSIQYTPEIGRGRGPVNHFAYLKKEGLDDELSK
HHHEEEEECCCCCCCCCCHHHHHHHHHCCCCHHHCC
>Mature Secondary Structure
MIKPKIALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVAQNTLGVQHIHNLNHQW
CCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHCCHHH
VDEQLDSVFNDTLPHAIKTGMIATADTMETIRHYLMQHESIPYVIDPVMLAKSGDSLMDN
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEECHHHEECCCCCHHCC
DTKQNLQHTLLPLADVVTPNLPEAEEITGLTIDSEEKIMQAGRIFINEIGSKGIIIKGGH
CHHHHHHHHHHHHHHHHCCCCCCHHHHCCCEECCHHHHHHHHHHHHHHCCCCCEEEECCC
SNDTDIAKDYLFTNEGVQTFENERFKTKHTHGTGCTFSAVITAELAKGRPLFEAVHKAKK
CCCHHHHHHHHHCCCCHHHHHCCCCEECCCCCCCCEEHHHHHHHHHCCCHHHHHHHHHHH
FISMSIQYTPEIGRGRGPVNHFAYLKKEGLDDELSK
HHHEEEEECCCCCCCCCCHHHHHHHHHCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11418146