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The map label for this gene is thiM [H]

Identifier: 156980408

GI number: 156980408

Start: 2225754

End: 2226545

Strand: Reverse

Name: thiM [H]

Synonym: SAHV_2077

Alternate gene names: 156980408

Gene position: 2226545-2225754 (Counterclockwise)

Preceding gene: 156980409

Following gene: 156980407

Centisome position: 77.31

GC content: 35.48

Gene sequence:

>792_bases
ATGAATTATCTAAATAAAATACGTATTGAAAACCCATTAACAATTTGTTATACAAACGATGTAGTTAAAAATTTTACAGC
GAATGGTTTATTAAGTATTGGTGCGAGCCCTGCCATGAGTGAAGCTCCCGAAGAAGCTGAAGAATTTTACAAAGTTGCAC
AAGCGCTATTAATCAATATCGGTACTTTAACAGCACAAAATGAACAAGATATTATTGCGATTGCTCAAACGGCAAATGAG
GCAGGCTTACCTATTGTATTTGACCCTGTAGCTGTTGGTGCTTCTACATATCGAAAGCAATTTTGTAAATTATTATTGAA
ATCAGCGAAAGTATCAGTAATTAAAGGCAATGCATCTGAAATATTAGCGTTGATTGATGATACAGCAACTATGAAAGGTA
CAGATAGTGATGCTAATCTTGATGCGGTTGCAATAGCGAAAAAGGCTTACGCAATATATAAAACTGCAATAGTAATCACA
GGTAAAGAGGACGTTATTGTTCAAGATAATAAAGCCATCGTATTAGCTAATGGATCTCCATTATTAGCACGAGTAACTGG
AGCTGGTTGTTTATTAGGAGGCGTTATTGCTGGATTTTTATTTAGAGAAACAGAACCAGACATAGAAGCGTTAATTGAAG
CGGTAAGCGTATTTAATATTGCTGCTGAGGTAGCTGCTGAAAATGAAAATTGTGGTGGTCCTGGTACGTTTTCACCATTG
TTGCTTGATACGTTATATCATTTAAATGAAACAACCTATCAACAACGGATTCGTATTCAAGAGGTGGAATAA

Upstream 100 bases:

>100_bases
AAAAAGTTTATTTCAATGAGTATACAATATACACCTGAAATCGGCCGTGGTAGAGGACCAGTGAATCATTTTGCATATTT
AAAGAAAGAGGGATTAGACG

Downstream 100 bases:

>100_bases
TATGTTTAACCAATCGTATCTAAATGTGTATTTTATTTGTGGGACCTCCGATGTTCCGAGTCATCGAACTATTCATGAAG
TATTAGAAGCAGCATTAAAG

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MNYLNKIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPEEAEEFYKVAQALLINIGTLTAQNEQDIIAIAQTANE
AGLPIVFDPVAVGASTYRKQFCKLLLKSAKVSVIKGNASEILALIDDTATMKGTDSDANLDAVAIAKKAYAIYKTAIVIT
GKEDVIVQDNKAIVLANGSPLLARVTGAGCLLGGVIAGFLFRETEPDIEALIEAVSVFNIAAEVAAENENCGGPGTFSPL
LLDTLYHLNETTYQQRIRIQEVE

Sequences:

>Translated_263_residues
MNYLNKIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPEEAEEFYKVAQALLINIGTLTAQNEQDIIAIAQTANE
AGLPIVFDPVAVGASTYRKQFCKLLLKSAKVSVIKGNASEILALIDDTATMKGTDSDANLDAVAIAKKAYAIYKTAIVIT
GKEDVIVQDNKAIVLANGSPLLARVTGAGCLLGGVIAGFLFRETEPDIEALIEAVSVFNIAAEVAAENENCGGPGTFSPL
LLDTLYHLNETTYQQRIRIQEVE
>Mature_263_residues
MNYLNKIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPEEAEEFYKVAQALLINIGTLTAQNEQDIIAIAQTANE
AGLPIVFDPVAVGASTYRKQFCKLLLKSAKVSVIKGNASEILALIDDTATMKGTDSDANLDAVAIAKKAYAIYKTAIVIT
GKEDVIVQDNKAIVLANGSPLLARVTGAGCLLGGVIAGFLFRETEPDIEALIEAVSVFNIAAEVAAENENCGGPGTFSPL
LLDTLYHLNETTYQQRIRIQEVE

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788421, Length=250, Percent_Identity=39.2, Blast_Score=159, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6325042, Length=275, Percent_Identity=31.6363636363636, Blast_Score=112, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000417 [H]

Pfam domain/function: PF02110 HK [H]

EC number: =2.7.1.50 [H]

Molecular weight: Translated: 28067; Mature: 28067

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYLNKIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPEEAEEFYKVAQALLINI
CCCCCCEEECCCEEEEEECHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
GTLTAQNEQDIIAIAQTANEAGLPIVFDPVAVGASTYRKQFCKLLLKSAKVSVIKGNASE
CCEECCCCCCEEEEEECCCCCCCCEEECHHHHCHHHHHHHHHHHHHHHCCEEEEECCHHH
ILALIDDTATMKGTDSDANLDAVAIAKKAYAIYKTAIVITGKEDVIVQDNKAIVLANGSP
EEEEECCCCCCCCCCCCCCCHHHHHHHHHHEEEEEEEEEECCCCEEEECCCEEEEECCCC
LLARVTGAGCLLGGVIAGFLFRETEPDIEALIEAVSVFNIAAEVAAENENCGGPGTFSPL
EEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
LLDTLYHLNETTYQQRIRIQEVE
HHHHHHHCCCHHHHHHEEEEECC
>Mature Secondary Structure
MNYLNKIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPEEAEEFYKVAQALLINI
CCCCCCEEECCCEEEEEECHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
GTLTAQNEQDIIAIAQTANEAGLPIVFDPVAVGASTYRKQFCKLLLKSAKVSVIKGNASE
CCEECCCCCCEEEEEECCCCCCCCEEECHHHHCHHHHHHHHHHHHHHHCCEEEEECCHHH
ILALIDDTATMKGTDSDANLDAVAIAKKAYAIYKTAIVITGKEDVIVQDNKAIVLANGSP
EEEEECCCCCCCCCCCCCCCHHHHHHHHHHEEEEEEEEEECCCCEEEECCCEEEEECCCC
LLARVTGAGCLLGGVIAGFLFRETEPDIEALIEAVSVFNIAAEVAAENENCGGPGTFSPL
EEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
LLDTLYHLNETTYQQRIRIQEVE
HHHHHHHCCCHHHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA