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The map label for this gene is nrgA [H]

Identifier: 156980359

GI number: 156980359

Start: 2164365

End: 2165615

Strand: Reverse

Name: nrgA [H]

Synonym: SAHV_2028

Alternate gene names: 156980359

Gene position: 2165615-2164365 (Counterclockwise)

Preceding gene: 156980360

Following gene: 156980358

Centisome position: 75.19

GC content: 32.85

Gene sequence:

>1251_bases
ATGAATCTTAACGATACGATATTTATGTTTTTGTGTACATTATTAGTTTGGTTAATGACACCAGGATTAAGTTTATTTTA
TGGTGGGTTAGTTCAATCTAAAAATGCGCTTAATACTGTCATGCAAAGTATGGCAGCAATTGTGCTTGTTACATTTGTAT
GGATAACAGTTGGTTTTACAATTAGTTTTGGGAATGGGAATTTATGGTTCGGAAATTGGGAATATACTTTTCTTAATCAT
GTAGGTTTTGCGACTCAAGAAGATATTAGCCCACATATTCCTTTCGCTTTGTTTATGTTATTTCAAATGATGTTTTGTAC
GATTGCAATTTCTATTTTATCTGGTTCAATCGCTGAGAAAATGAAGTTTATTCCTTATTTATTATTCGTAGTAATATGGA
CTGCTCTTGTATACAGTCCAGTAGCACATTGGGTTTGGGGCGGCGGTTGGATTAATAAACTCGGTGTATTAGATTTCGCT
GGAGGTACGGTTGTTCATATTACATCAGGTGTTTCTGGTTTAGTATTAGCTATTATGATTGGAAAAGGAAACAAACATTC
TGAATCAACACCACATAATCTTATCATTACGTTGATTGGCGGTATATTCGTGTGGATTGGTTGGTATGGATTTAATGTAG
GTAGTGCTTTTACATTTGATAATATTGCGATGCTTGCATTTACAAATACTGTCATTTCAGCCAGTGCAGGTGCTATAGGT
TGGTTAATTTTAGAATATATTTTTAAAAAGACGACAAGTTTACTTGGACTTTTACTCGGTGCATTAGCAGGATTAGTTGT
TATTACTCCTGCAGCAGGATATGTAACATATCTTAGTGCAACAATAATGGCTTTAATAGGAGGTATCTGTTGTTATATTG
TCATTAATTACATCAAGGTAAAACTAAAATATCATGATGCATTAGATGCATTTGGTATTCATGGTGTTGGTGGTATTATT
GGTGCTGTTTTAACAGCAGTTTTCCAAAGTAAAAAAGCCAATCCTGACATTGAGAATGGCTTTATTTATACTGGTGACAT
ACATATTATACTTGTACAAATATTATGTGTAACAGCAGTTGTAATTTTTAGTATCGTCATGACGTTTATTATTGCGAAAG
TAATTAAATTAATTACACCATTATCTGTTACGGAACAAGAAACGAATATAGGATTAGACAAGATTGTTCACGGTGAACAT
GCTTACTTTGAAGGTGAGCTAAATAGATTCAATAAACATATTCGATATTAG

Upstream 100 bases:

>100_bases
ATAGTGTGGCATATTTTATTTCAAAAGATGTAATAAAACTTAACGCATTTTTGCTTTTTATAAATTGTCAGATTATTATG
AAAACAAGGGAGTGGTAAGT

Downstream 100 bases:

>100_bases
AATATATTTACATAGAATATTCATTGTCCTGACATTTAACTAAAGGTTGATGTTGGGACATTTTGTTATACAAAAGTTTT
ATTTTGAAATCTTTTTATGA

Product: probabale ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Membrane protein NrgA; Protein AmtB [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSMAAIVLVTFVWITVGFTISFGNGNLWFGNWEYTFLNH
VGFATQEDISPHIPFALFMLFQMMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFA
GGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFTFDNIAMLAFTNTVISASAGAIG
WLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVTYLSATIMALIGGICCYIVINYIKVKLKYHDALDAFGIHGVGGII
GAVLTAVFQSKKANPDIENGFIYTGDIHIILVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGLDKIVHGEH
AYFEGELNRFNKHIRY

Sequences:

>Translated_416_residues
MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSMAAIVLVTFVWITVGFTISFGNGNLWFGNWEYTFLNH
VGFATQEDISPHIPFALFMLFQMMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFA
GGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFTFDNIAMLAFTNTVISASAGAIG
WLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVTYLSATIMALIGGICCYIVINYIKVKLKYHDALDAFGIHGVGGII
GAVLTAVFQSKKANPDIENGFIYTGDIHIILVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGLDKIVHGEH
AYFEGELNRFNKHIRY
>Mature_416_residues
MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSMAAIVLVTFVWITVGFTISFGNGNLWFGNWEYTFLNH
VGFATQEDISPHIPFALFMLFQMMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFA
GGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFTFDNIAMLAFTNTVISASAGAIG
WLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVTYLSATIMALIGGICCYIVINYIKVKLKYHDALDAFGIHGVGGII
GAVLTAVFQSKKANPDIENGFIYTGDIHIILVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGLDKIVHGEH
AYFEGELNRFNKHIRY

Specific function: Functions as an ammonium and methylammonium transporter in the absence of glutamine. Required for ammonium utilization at low concentrations or at low pH values, when ammonium is the single nitrogen source. Required for binding of NrgB to the membrane [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=403, Percent_Identity=40.1985111662531, Blast_Score=278, Evalue=4e-76,
Organism=Caenorhabditis elegans, GI133931100, Length=427, Percent_Identity=25.0585480093677, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI32564042, Length=416, Percent_Identity=26.2019230769231, Blast_Score=121, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17550182, Length=438, Percent_Identity=27.3972602739726, Blast_Score=117, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17550180, Length=431, Percent_Identity=25.5220417633411, Blast_Score=115, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6324187, Length=412, Percent_Identity=33.7378640776699, Blast_Score=214, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6325396, Length=416, Percent_Identity=32.6923076923077, Blast_Score=212, Evalue=8e-56,
Organism=Saccharomyces cerevisiae, GI6321559, Length=416, Percent_Identity=32.4519230769231, Blast_Score=211, Evalue=3e-55,
Organism=Drosophila melanogaster, GI161078314, Length=414, Percent_Identity=28.5024154589372, Blast_Score=128, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 45480; Mature: 45480

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSMAAIVLVTFVWITVGFT
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHE
ISFGNGNLWFGNWEYTFLNHVGFATQEDISPHIPFALFMLFQMMFCTIAISILSGSIAEK
EEECCCEEEECCCEEHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFAGGTVVHITSGVSGLVLAIMI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCEEECCCEEEEEECCHHHHHHHEEE
GKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFTFDNIAMLAFTNTVISASAGAIG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHCCCHHHH
WLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVTYLSATIMALIGGICCYIVINYIKV
HHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLKYHDALDAFGIHGVGGIIGAVLTAVFQSKKANPDIENGFIYTGDIHIILVQILCVTAV
EEEEHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHHHHHHH
VIFSIVMTFIIAKVIKLITPLSVTEQETNIGLDKIVHGEHAYFEGELNRFNKHIRY
HHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCHHHHCCCCCCEEHHHHHHHHHHCCC
>Mature Secondary Structure
MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSMAAIVLVTFVWITVGFT
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHE
ISFGNGNLWFGNWEYTFLNHVGFATQEDISPHIPFALFMLFQMMFCTIAISILSGSIAEK
EEECCCEEEECCCEEHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFAGGTVVHITSGVSGLVLAIMI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCEEECCCEEEEEECCHHHHHHHEEE
GKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFTFDNIAMLAFTNTVISASAGAIG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHCCCHHHH
WLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVTYLSATIMALIGGICCYIVINYIKV
HHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLKYHDALDAFGIHGVGGIIGAVLTAVFQSKKANPDIENGFIYTGDIHIILVQILCVTAV
EEEEHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHHHHHHH
VIFSIVMTFIIAKVIKLITPLSVTEQETNIGLDKIVHGEHAYFEGELNRFNKHIRY
HHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCHHHHCCCCCCEEHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8282685; 9353933; 9384377 [H]