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The map label for this gene is scrR [H]

Identifier: 156980358

GI number: 156980358

Start: 2163232

End: 2164182

Strand: Reverse

Name: scrR [H]

Synonym: SAHV_2027

Alternate gene names: 156980358

Gene position: 2164182-2163232 (Counterclockwise)

Preceding gene: 156980359

Following gene: 156980357

Centisome position: 75.14

GC content: 32.6

Gene sequence:

>951_bases
ATGAAAAATATATCTGATATTGCCAAATTGGCAGGCGTTTCAAAAAGCACAGTATCTAGATTTTTAAATAATGGATCTGT
CAGTAAAAAAACAAGTGAAAAATTAACAAGAATTATAGCAGAACATGACTATCAACCGAATCAATTTGCTCAAAGTTTAA
GAGCGAGACAAACACACCTTATTGGTGCCATTATACCTAGAATGAATTCATATGCAGTAGATGAAACAATCAAAGGATTG
GCAAAACAATGCCAAAAATATGAATCACAATTAATTTTAAATTACACAGGTTTAAATATCGAAGCAGAAATACAAGCGCT
TGAAACATTAGCACGCAGTAAAGTAGATGGTATTGTTTTAATGGCTACAGACATAACAGAGAGACATATTGAAGTCATTA
ATAAAATGAATGTACCAATCGTTATTGTTGGTCAACAACATGAACAACTTCATAGTATTGTGCATGATGATTATAAAGCA
GGTCAAATTATAGGCGAATGGATTGGTCAACAGGGATATCAACAAGTTGAAGTGTTTAGTGTAAGTGAAAAAGATATTGC
AGTTGGTATACATAGAAAACGTGGTTTACTTGACCAGTTAGCTAAATACCAAATTAAACCTAATATACATGAAACAAATT
TTACTTATGTGGAAGCACAAAAAGATGTTGCAAATGTTTTGGAAAATGTGGAGCAAGTAGATGCGGTTGTTGGAGCAACT
GATACGATTGCATTAGCTGCCTATAAATATTATTCTGATAAAAATGATGTTATGAAACCACATCAAATATATGGTTTTGG
TGGTGACCCAATGACACAATTAGTGTCTCCATCGATAAAAACAATTCATTATAATTATTTTGAAGCTGGCCAATGCGCGA
TGGAAGAGATACAACAGATGCTTAAAAAGCAAGATATGCCATATAGCGTCACAGTAGATGTTAATATTTAG

Upstream 100 bases:

>100_bases
TTTGAAATCTTTTTATGAAAGAAGCAGAAATATTATTTAAAGCGGTTACACATATGCTAAAATAAGGCTAAGTGTCACAA
ATAATGATAGGTGAATAAGT

Downstream 100 bases:

>100_bases
ACGCTGTATTTTTTAAAACAAATGTGGAACCGATACCATATAACTATAAATGGATAGGTTAAAAGTTAAAGAACGTAGGT
AAAATTTGCTATAATAGAAT

Product: sucrose operon repressor

Products: NA

Alternate protein names: Scr operon regulatory protein [H]

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MKNISDIAKLAGVSKSTVSRFLNNGSVSKKTSEKLTRIIAEHDYQPNQFAQSLRARQTHLIGAIIPRMNSYAVDETIKGL
AKQCQKYESQLILNYTGLNIEAEIQALETLARSKVDGIVLMATDITERHIEVINKMNVPIVIVGQQHEQLHSIVHDDYKA
GQIIGEWIGQQGYQQVEVFSVSEKDIAVGIHRKRGLLDQLAKYQIKPNIHETNFTYVEAQKDVANVLENVEQVDAVVGAT
DTIALAAYKYYSDKNDVMKPHQIYGFGGDPMTQLVSPSIKTIHYNYFEAGQCAMEEIQQMLKKQDMPYSVTVDVNI

Sequences:

>Translated_316_residues
MKNISDIAKLAGVSKSTVSRFLNNGSVSKKTSEKLTRIIAEHDYQPNQFAQSLRARQTHLIGAIIPRMNSYAVDETIKGL
AKQCQKYESQLILNYTGLNIEAEIQALETLARSKVDGIVLMATDITERHIEVINKMNVPIVIVGQQHEQLHSIVHDDYKA
GQIIGEWIGQQGYQQVEVFSVSEKDIAVGIHRKRGLLDQLAKYQIKPNIHETNFTYVEAQKDVANVLENVEQVDAVVGAT
DTIALAAYKYYSDKNDVMKPHQIYGFGGDPMTQLVSPSIKTIHYNYFEAGQCAMEEIQQMLKKQDMPYSVTVDVNI
>Mature_316_residues
MKNISDIAKLAGVSKSTVSRFLNNGSVSKKTSEKLTRIIAEHDYQPNQFAQSLRARQTHLIGAIIPRMNSYAVDETIKGL
AKQCQKYESQLILNYTGLNIEAEIQALETLARSKVDGIVLMATDITERHIEVINKMNVPIVIVGQQHEQLHSIVHDDYKA
GQIIGEWIGQQGYQQVEVFSVSEKDIAVGIHRKRGLLDQLAKYQIKPNIHETNFTYVEAQKDVANVLENVEQVDAVVGAT
DTIALAAYKYYSDKNDVMKPHQIYGFGGDPMTQLVSPSIKTIHYNYFEAGQCAMEEIQQMLKKQDMPYSVTVDVNI

Specific function: Negative regulator of scrB expression [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790689, Length=307, Percent_Identity=27.0358306188925, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI1787580, Length=326, Percent_Identity=25.7668711656442, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1789068, Length=279, Percent_Identity=27.2401433691756, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1790194, Length=303, Percent_Identity=24.4224422442244, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1787948, Length=324, Percent_Identity=24.0740740740741, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790715, Length=320, Percent_Identity=24.6875, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788474, Length=306, Percent_Identity=23.8562091503268, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786268, Length=221, Percent_Identity=26.6968325791855, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI48994940, Length=308, Percent_Identity=22.4025974025974, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1789202, Length=291, Percent_Identity=23.7113402061856, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786540, Length=315, Percent_Identity=24.4444444444444, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787906, Length=256, Percent_Identity=24.21875, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790369, Length=301, Percent_Identity=21.2624584717608, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35486; Mature: 35486

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNISDIAKLAGVSKSTVSRFLNNGSVSKKTSEKLTRIIAEHDYQPNQFAQSLRARQTHL
CCCHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
IGAIIPRMNSYAVDETIKGLAKQCQKYESQLILNYTGLNIEAEIQALETLARSKVDGIVL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHCCCCEEE
MATDITERHIEVINKMNVPIVIVGQQHEQLHSIVHDDYKAGQIIGEWIGQQGYQQVEVFS
EECCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEE
VSEKDIAVGIHRKRGLLDQLAKYQIKPNIHETNFTYVEAQKDVANVLENVEQVDAVVGAT
ECCCCEEEEEHHHCCHHHHHHHHCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCC
DTIALAAYKYYSDKNDVMKPHQIYGFGGDPMTQLVSPSIKTIHYNYFEAGQCAMEEIQQM
HHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHCCCHHEEEECCHHHHHHHHHHHHHH
LKKQDMPYSVTVDVNI
HHHCCCCEEEEEEEEC
>Mature Secondary Structure
MKNISDIAKLAGVSKSTVSRFLNNGSVSKKTSEKLTRIIAEHDYQPNQFAQSLRARQTHL
CCCHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
IGAIIPRMNSYAVDETIKGLAKQCQKYESQLILNYTGLNIEAEIQALETLARSKVDGIVL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHCCCCEEE
MATDITERHIEVINKMNVPIVIVGQQHEQLHSIVHDDYKAGQIIGEWIGQQGYQQVEVFS
EECCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEE
VSEKDIAVGIHRKRGLLDQLAKYQIKPNIHETNFTYVEAQKDVANVLENVEQVDAVVGAT
ECCCCEEEEEHHHCCHHHHHHHHCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCC
DTIALAAYKYYSDKNDVMKPHQIYGFGGDPMTQLVSPSIKTIHYNYFEAGQCAMEEIQQM
HHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHCCCHHEEEECCHHHHHHHHHHHHHH
LKKQDMPYSVTVDVNI
HHHCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550467 [H]