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The map label for this gene is hemN [H]

Identifier: 156937134

GI number: 156937134

Start: 297469

End: 298623

Strand: Reverse

Name: hemN [H]

Synonym: Igni_0340

Alternate gene names: 156937134

Gene position: 298623-297469 (Counterclockwise)

Preceding gene: 156937135

Following gene: 156937132

Centisome position: 23.01

GC content: 58.96

Gene sequence:

>1155_bases
ATGGTAGCGTTGTGGAGAGCCTTCAGCGCTCTCGCGGAGCTCTCGAGGAGGTGGAGCGAGGCTTGGTTGAGGAAGCTCCT
ATCCTCCCCCGGGACCCCCATGAGGCCGGAAGGGAGGGTTTCCCTATACTTACACGTGCCGTTTTGTAGGTCCCTCTGCA
AGTTCTGCCACTTCGTCCGGTTTCCCTACTCCGAGGACTTGGCTTCTAAGTACTTCAAGAAGTTGATTAAGGACGTGGAG
GAGGCTCACTCCAGCGGAGTTGAGGCGGTGGAGGTCTACGTCGGCGGGGGGTCGCCATCCTCCAAGCCGGAAGCCTTAGG
GGAGCTGGTGGATCTGGTATGGTCCCTCTGGAGGCCCCAGATCTCCGTAGAGGTCCACCCAGTGGACGTGGCCTTCTTGG
GGGCCGCAGAACACTTGGACCCGAAGAAGGTAACGAGGGTGAGCATGGGGGTTCAGAGCTTCTCCCCGGAAGGGTTGAAA
AAGCTCGGGAGGCCAGTCTCCCCCGAAACTAACGAGCTCGCAATAGAAATTTTAAAATCTAAGAAGTTCAAGACCTTTAA
CGTGGACGTGGTGTGGGGCTTGGAGGACCCCACCCCCGACGCGGTTAAGGCCTTGGAGTGCGGAGCCGACCAAGTGACCT
TCTACCCGCTCATGCCCTTCCCCAAGAGAGGGCCGGAGGGAGAGAGGCTGTGCTTCCAGAACTACCAGAGGATAGTGAGG
GAGGCCTCCAAGAGGGGGTTCGTTAGGCAGAACGCTTGGACCTTCTCTAAGACAGAGGCCATGGTCGACGAGTACGTAGC
TCGCGCGGAGGAATTCTTGGGCTTGGGAGTGAGCTCTTTCAGTTTGGTAGGAGGAGCAGCTCACTTGAACGTATTCGACT
TGGAGGAGTACTTTAAGAGGGGTTGGTCGGAGCCTTGGCACTCGGTCAAGCTCAACCCCCTGGAGGGGTTGGCGTTTAAG
GTAGCTATGGCGCTTCACTCGTCCCCTCCGCTGAGGGCCGAGGCGCTCTGGTACTCCGGGCTCGTGGCCTTGAGGGAGCT
CTACGGCGTCTTGGGGGAGTTCAGGCTTTCTGTGGTGAGCAAGCTCTGGGAGAAGTACGCGCCGAAACCCTCGAGAGGAA
GCGCCTCATTGCCCGCTCGAGGTCAGAGAGCGTGA

Upstream 100 bases:

>100_bases
CAATAGTAATGGAGCTCGCCGAGATGAGGGGTTTCTTGGGCTGAGCCCTAACTTTCTGGTCAATCACAGTTATCTAAACC
GACCGGACGGGAAAGAGTCG

Downstream 100 bases:

>100_bases
AGAAGCCCTCTATCCTCTTGACTTCCTTGTCGTCGTGATAGAAGACCACGGTGGGGACCCAGCTTACCCCCAAGCTCTTG
AAGAGCTTCGCGGTCTCCGG

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MVALWRAFSALAELSRRWSEAWLRKLLSSPGTPMRPEGRVSLYLHVPFCRSLCKFCHFVRFPYSEDLASKYFKKLIKDVE
EAHSSGVEAVEVYVGGGSPSSKPEALGELVDLVWSLWRPQISVEVHPVDVAFLGAAEHLDPKKVTRVSMGVQSFSPEGLK
KLGRPVSPETNELAIEILKSKKFKTFNVDVVWGLEDPTPDAVKALECGADQVTFYPLMPFPKRGPEGERLCFQNYQRIVR
EASKRGFVRQNAWTFSKTEAMVDEYVARAEEFLGLGVSSFSLVGGAAHLNVFDLEEYFKRGWSEPWHSVKLNPLEGLAFK
VAMALHSSPPLRAEALWYSGLVALRELYGVLGEFRLSVVSKLWEKYAPKPSRGSASLPARGQRA

Sequences:

>Translated_384_residues
MVALWRAFSALAELSRRWSEAWLRKLLSSPGTPMRPEGRVSLYLHVPFCRSLCKFCHFVRFPYSEDLASKYFKKLIKDVE
EAHSSGVEAVEVYVGGGSPSSKPEALGELVDLVWSLWRPQISVEVHPVDVAFLGAAEHLDPKKVTRVSMGVQSFSPEGLK
KLGRPVSPETNELAIEILKSKKFKTFNVDVVWGLEDPTPDAVKALECGADQVTFYPLMPFPKRGPEGERLCFQNYQRIVR
EASKRGFVRQNAWTFSKTEAMVDEYVARAEEFLGLGVSSFSLVGGAAHLNVFDLEEYFKRGWSEPWHSVKLNPLEGLAFK
VAMALHSSPPLRAEALWYSGLVALRELYGVLGEFRLSVVSKLWEKYAPKPSRGSASLPARGQRA
>Mature_384_residues
MVALWRAFSALAELSRRWSEAWLRKLLSSPGTPMRPEGRVSLYLHVPFCRSLCKFCHFVRFPYSEDLASKYFKKLIKDVE
EAHSSGVEAVEVYVGGGSPSSKPEALGELVDLVWSLWRPQISVEVHPVDVAFLGAAEHLDPKKVTRVSMGVQSFSPEGLK
KLGRPVSPETNELAIEILKSKKFKTFNVDVVWGLEDPTPDAVKALECGADQVTFYPLMPFPKRGPEGERLCFQNYQRIVR
EASKRGFVRQNAWTFSKTEAMVDEYVARAEEFLGLGVSSFSLVGGAAHLNVFDLEEYFKRGWSEPWHSVKLNPLEGLAFK
VAMALHSSPPLRAEALWYSGLVALRELYGVLGEFRLSVVSKLWEKYAPKPSRGSASLPARGQRA

Specific function: Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX [H]

COG id: COG0635

COG function: function code H; Coproporphyrinogen III oxidase and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anaerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789325, Length=199, Percent_Identity=30.6532663316583, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI87082341, Length=306, Percent_Identity=23.8562091503268, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR004558
- InterPro:   IPR010723
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06969 HemN_C; PF04055 Radical_SAM [H]

EC number: =1.3.99.22 [H]

Molecular weight: Translated: 43024; Mature: 43024

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVALWRAFSALAELSRRWSEAWLRKLLSSPGTPMRPEGRVSLYLHVPFCRSLCKFCHFVR
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHC
FPYSEDLASKYFKKLIKDVEEAHSSGVEAVEVYVGGGSPSSKPEALGELVDLVWSLWRPQ
CCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCC
ISVEVHPVDVAFLGAAEHLDPKKVTRVSMGVQSFSPEGLKKLGRPVSPETNELAIEILKS
EEEEEEEECEEEECCHHCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHC
KKFKTFNVDVVWGLEDPTPDAVKALECGADQVTFYPLMPFPKRGPEGERLCFQNYQRIVR
CCCEEEEEEEEECCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHH
EASKRGFVRQNAWTFSKTEAMVDEYVARAEEFLGLGVSSFSLVGGAAHLNVFDLEEYFKR
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHCEEECHHHHHHC
GWSEPWHSVKLNPLEGLAFKVAMALHSSPPLRAEALWYSGLVALRELYGVLGEFRLSVVS
CCCCCCCCEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLWEKYAPKPSRGSASLPARGQRA
HHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVALWRAFSALAELSRRWSEAWLRKLLSSPGTPMRPEGRVSLYLHVPFCRSLCKFCHFVR
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHC
FPYSEDLASKYFKKLIKDVEEAHSSGVEAVEVYVGGGSPSSKPEALGELVDLVWSLWRPQ
CCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCC
ISVEVHPVDVAFLGAAEHLDPKKVTRVSMGVQSFSPEGLKKLGRPVSPETNELAIEILKS
EEEEEEEECEEEECCHHCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHC
KKFKTFNVDVVWGLEDPTPDAVKALECGADQVTFYPLMPFPKRGPEGERLCFQNYQRIVR
CCCEEEEEEEEECCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHH
EASKRGFVRQNAWTFSKTEAMVDEYVARAEEFLGLGVSSFSLVGGAAHLNVFDLEEYFKR
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHCEEECHHHHHHC
GWSEPWHSVKLNPLEGLAFKVAMALHSSPPLRAEALWYSGLVALRELYGVLGEFRLSVVS
CCCCCCCCEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLWEKYAPKPSRGSASLPARGQRA
HHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8809776 [H]