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The map label for this gene is pyrG [H]

Identifier: 156937125

GI number: 156937125

Start: 291016

End: 292575

Strand: Reverse

Name: pyrG [H]

Synonym: Igni_0331

Alternate gene names: 156937125

Gene position: 292575-291016 (Counterclockwise)

Preceding gene: 156937126

Following gene: 156937124

Centisome position: 22.55

GC content: 57.5

Gene sequence:

>1560_bases
GTGCTCTCCAGCCTAGGGAAGGGTATCCTAACGGCATCCACAGCTCTGTTTTTCAAGTCCATGGGATACACGACCACCGC
GATAAAGATAGACCCGTACGTGAACGTGGACGCGAGCACGATGAACCCCTACGCCCACGGGGAGGTCTTCGTGACCGAGG
ACGGCGGAGAGACCGACTTGGACTTGGGTCACTACGAGAGGTTCTTACATCAGAACTTGACCAAGAATAACAACATAACT
ACCGGGAAAGTCTACTTGAACGTCATTGAGAAGGAGAGGAAGGGGGAGTACTTAGGGCAGACCGTCCAAATAATTCCCCA
CGTGACGGACGAAATTAAAAGGATGATAGACGAAGTGGCCAAGGACTTCGACGTAGCCGTGGTAGAGATAGGGGGCACGG
TGGGCGACATAGAGGGGCTCCCCTTCCTGGAGGCCGCCCGCCAGATGTGGGTGGAGAGGAGGGGCGACGTCGCCTTCGTC
CACGTGGCCTTGGTGCCGGTGCTAAGCACCACGGGAGAGCAGAAGACCAAGCCGCTCCAGCACTCGGTACAAGAGCTGAG
GAGGATAGGCATCCAGCCCCACGTGGTGGTCGGACGCTCCACCAAGCCCTTGGAGCCCGAGACCAAGAGGAAGATAGCCC
TCTACTCCAACTTGCCGGAGGAGGCGGTGTTCTCCGACCCGGACGTGGAGTTCGTCTACGAGGTGCCCCTAAGGCTGGCG
GAGCAGGGGTACCCCAAGTACCTCACCAAGATATTGGGATTGGAAGAGAGGAAGATAGAACTCGAATATTGGGTAGATTT
CGTTAACAAGTTGAAGTCCTTACCGGAGGGGCCCACGGTAGCTATGGTCGGTAAGTACACGAAATTGAAGGACAGCTACT
TGAGCATCATAGAGTCCTTAAAACACTCGGCCGTAGAGGCGGGGTTCATGCCCAAGCTGAAGTGGGTGGAGGTGACGGAC
GTGGAGAGGGGCAAGCTGAGCCCGGAGGAGGCCGCGGAGGCGGACGGGGCGATAATCTTGCCCGGCTTCGGGAAGAGGGG
GGCGGAGGGGAAGATAGCAGTCATAAAGGAGCTAAGGGAGAGGGGTGTGCCCACCTTGGGAATATGCTTCGGGATGCAGA
TGATGGTGGTTGAGGTAGCTAGGCACTTGGCCGGCCTGGAGGGCGCGAACAGCGAGGAGGTAGACCCCAACACCCCCCAC
CCCGTAGTGGCCTTGCTGGACGAACAGAGGAAGCTGAAGTACTTGGGCGGCACCATGAGGTTGGGAGCGAAGCCAGTAAT
AATAATGAAAGGGACCAAGCTCTGGGAGGCCTACAAGAAGACGTTGGTTAAAGAAAGGCATAGACATAGGTACGACGTTA
ACCCAAAGTACTTGGACAAGTTAGAGGAGGCGGGACTAAGGGTATCGGCGTGGAGCGAGGGAGTGCCGGAGGGGGTGGAG
CTGGAGGGCCAAGAGTTCTGGGGGGTCCAGTACCACCCCGAGTTCAAGAGCAGGCCGTTGCAGCCCTCGCCGGTCTACAC
GCGCTTCTTGAAGACCCTCAAGTTCTCTGCTTCCTCTTGA

Upstream 100 bases:

>100_bases
GACCCCTTGGAGGACTCCACCGAAGCGGTGCCGTAAAAAAGGGCTCAGTCGAAGGGGCGGCGGGCTGAAGCTGACAAAGT
TCGTTTTCATAACCGGCGGG

Downstream 100 bases:

>100_bases
CCCCGTTCCTCCCGTTGCTCGAATGGCCCTCCGTGAAGGCTTTCTATCCTCTTTTGAGACTCTACTCTTCATAAAAGACC
CTGAAACAAAGAGTGTCACA

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MLSSLGKGILTASTALFFKSMGYTTTAIKIDPYVNVDASTMNPYAHGEVFVTEDGGETDLDLGHYERFLHQNLTKNNNIT
TGKVYLNVIEKERKGEYLGQTVQIIPHVTDEIKRMIDEVAKDFDVAVVEIGGTVGDIEGLPFLEAARQMWVERRGDVAFV
HVALVPVLSTTGEQKTKPLQHSVQELRRIGIQPHVVVGRSTKPLEPETKRKIALYSNLPEEAVFSDPDVEFVYEVPLRLA
EQGYPKYLTKILGLEERKIELEYWVDFVNKLKSLPEGPTVAMVGKYTKLKDSYLSIIESLKHSAVEAGFMPKLKWVEVTD
VERGKLSPEEAAEADGAIILPGFGKRGAEGKIAVIKELRERGVPTLGICFGMQMMVVEVARHLAGLEGANSEEVDPNTPH
PVVALLDEQRKLKYLGGTMRLGAKPVIIMKGTKLWEAYKKTLVKERHRHRYDVNPKYLDKLEEAGLRVSAWSEGVPEGVE
LEGQEFWGVQYHPEFKSRPLQPSPVYTRFLKTLKFSASS

Sequences:

>Translated_519_residues
MLSSLGKGILTASTALFFKSMGYTTTAIKIDPYVNVDASTMNPYAHGEVFVTEDGGETDLDLGHYERFLHQNLTKNNNIT
TGKVYLNVIEKERKGEYLGQTVQIIPHVTDEIKRMIDEVAKDFDVAVVEIGGTVGDIEGLPFLEAARQMWVERRGDVAFV
HVALVPVLSTTGEQKTKPLQHSVQELRRIGIQPHVVVGRSTKPLEPETKRKIALYSNLPEEAVFSDPDVEFVYEVPLRLA
EQGYPKYLTKILGLEERKIELEYWVDFVNKLKSLPEGPTVAMVGKYTKLKDSYLSIIESLKHSAVEAGFMPKLKWVEVTD
VERGKLSPEEAAEADGAIILPGFGKRGAEGKIAVIKELRERGVPTLGICFGMQMMVVEVARHLAGLEGANSEEVDPNTPH
PVVALLDEQRKLKYLGGTMRLGAKPVIIMKGTKLWEAYKKTLVKERHRHRYDVNPKYLDKLEEAGLRVSAWSEGVPEGVE
LEGQEFWGVQYHPEFKSRPLQPSPVYTRFLKTLKFSASS
>Mature_519_residues
MLSSLGKGILTASTALFFKSMGYTTTAIKIDPYVNVDASTMNPYAHGEVFVTEDGGETDLDLGHYERFLHQNLTKNNNIT
TGKVYLNVIEKERKGEYLGQTVQIIPHVTDEIKRMIDEVAKDFDVAVVEIGGTVGDIEGLPFLEAARQMWVERRGDVAFV
HVALVPVLSTTGEQKTKPLQHSVQELRRIGIQPHVVVGRSTKPLEPETKRKIALYSNLPEEAVFSDPDVEFVYEVPLRLA
EQGYPKYLTKILGLEERKIELEYWVDFVNKLKSLPEGPTVAMVGKYTKLKDSYLSIIESLKHSAVEAGFMPKLKWVEVTD
VERGKLSPEEAAEADGAIILPGFGKRGAEGKIAVIKELRERGVPTLGICFGMQMMVVEVARHLAGLEGANSEEVDPNTPH
PVVALLDEQRKLKYLGGTMRLGAKPVIIMKGTKLWEAYKKTLVKERHRHRYDVNPKYLDKLEEAGLRVSAWSEGVPEGVE
LEGQEFWGVQYHPEFKSRPLQPSPVYTRFLKTLKFSASS

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=537, Percent_Identity=45.8100558659218, Blast_Score=442, Evalue=1e-124,
Organism=Homo sapiens, GI28559085, Length=536, Percent_Identity=42.5373134328358, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI28559083, Length=536, Percent_Identity=42.5373134328358, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI221316689, Length=536, Percent_Identity=42.5373134328358, Blast_Score=422, Evalue=1e-118,
Organism=Escherichia coli, GI1789142, Length=528, Percent_Identity=45.2651515151515, Blast_Score=436, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI25148299, Length=589, Percent_Identity=36.6723259762309, Blast_Score=366, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6319432, Length=541, Percent_Identity=42.6987060998152, Blast_Score=398, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6322563, Length=542, Percent_Identity=41.1439114391144, Blast_Score=395, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24664469, Length=532, Percent_Identity=42.6691729323308, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI21357815, Length=485, Percent_Identity=42.0618556701031, Blast_Score=366, Evalue=1e-101,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 58104; Mature: 58104

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSSLGKGILTASTALFFKSMGYTTTAIKIDPYVNVDASTMNPYAHGEVFVTEDGGETDL
CCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCEECCCCCCCCCCCCEEEEECCCCCCCC
DLGHYERFLHQNLTKNNNITTGKVYLNVIEKERKGEYLGQTVQIIPHVTDEIKRMIDEVA
CHHHHHHHHHHHCCCCCCEEEHHEEEEEHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHH
KDFDVAVVEIGGTVGDIEGLPFLEAARQMWVERRGDVAFVHVALVPVLSTTGEQKTKPLQ
HCCCEEEEEECCCEECCCCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCHHCCHHH
HSVQELRRIGIQPHVVVGRSTKPLEPETKRKIALYSNLPEEAVFSDPDVEFVYEVPLRLA
HHHHHHHHCCCCCEEEECCCCCCCCCHHHCEEEEECCCCHHHHCCCCCCEEEEHHHHHHH
EQGYPKYLTKILGLEERKIELEYWVDFVNKLKSLPEGPTVAMVGKYTKLKDSYLSIIESL
HCCCHHHHHHHHCCCHHEEHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHH
KHSAVEAGFMPKLKWVEVTDVERGKLSPEEAAEADGAIILPGFGKRGAEGKIAVIKELRE
HHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHH
RGVPTLGICFGMQMMVVEVARHLAGLEGANSEEVDPNTPHPVVALLDEQRKLKYLGGTMR
CCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCHHHHHHCCCEEE
LGAKPVIIMKGTKLWEAYKKTLVKERHRHRYDVNPKYLDKLEEAGLRVSAWSEGVPEGVE
CCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCE
LEGQEFWGVQYHPEFKSRPLQPSPVYTRFLKTLKFSASS
ECCCEEECEEECCHHHCCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLSSLGKGILTASTALFFKSMGYTTTAIKIDPYVNVDASTMNPYAHGEVFVTEDGGETDL
CCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCEECCCCCCCCCCCCEEEEECCCCCCCC
DLGHYERFLHQNLTKNNNITTGKVYLNVIEKERKGEYLGQTVQIIPHVTDEIKRMIDEVA
CHHHHHHHHHHHCCCCCCEEEHHEEEEEHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHH
KDFDVAVVEIGGTVGDIEGLPFLEAARQMWVERRGDVAFVHVALVPVLSTTGEQKTKPLQ
HCCCEEEEEECCCEECCCCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCHHCCHHH
HSVQELRRIGIQPHVVVGRSTKPLEPETKRKIALYSNLPEEAVFSDPDVEFVYEVPLRLA
HHHHHHHHCCCCCEEEECCCCCCCCCHHHCEEEEECCCCHHHHCCCCCCEEEEHHHHHHH
EQGYPKYLTKILGLEERKIELEYWVDFVNKLKSLPEGPTVAMVGKYTKLKDSYLSIIESL
HCCCHHHHHHHHCCCHHEEHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHH
KHSAVEAGFMPKLKWVEVTDVERGKLSPEEAAEADGAIILPGFGKRGAEGKIAVIKELRE
HHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHH
RGVPTLGICFGMQMMVVEVARHLAGLEGANSEEVDPNTPHPVVALLDEQRKLKYLGGTMR
CCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCHHHHHHCCCEEE
LGAKPVIIMKGTKLWEAYKKTLVKERHRHRYDVNPKYLDKLEEAGLRVSAWSEGVPEGVE
CCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCE
LEGQEFWGVQYHPEFKSRPLQPSPVYTRFLKTLKFSASS
ECCCEEECEEECCHHHCCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]