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The map label for this gene is hemB [H]

Identifier: 156937118

GI number: 156937118

Start: 280797

End: 281825

Strand: Reverse

Name: hemB [H]

Synonym: Igni_0324

Alternate gene names: 156937118

Gene position: 281825-280797 (Counterclockwise)

Preceding gene: 156937124

Following gene: 156937116

Centisome position: 21.72

GC content: 60.45

Gene sequence:

>1029_bases
TTGAGCTTCCCCAGGCTCCGGCCGCGCAGGCTGAGGAAGAACTTGTACCTCAGGGACTTGGTCGCCGAGACCAGGCTCTC
CCCCAGGGACTTCATATACCCCATATTCGTCAAAGAAGGCATAGACAAGCCTGAAGAGATAAGCGCGATGCCCGGGCAGA
TGAGGTGGCCGGTGGGAGAGGAGTTAGTGAAGCACGTCCAAGAGGCGTTGGACCTAGGGGTAAGGTCGGTGCTGCTCTTC
GGGGTCCCCAAGCACAAGGACGACCTCGGCTCCTCCGCTTACGACGACCAAGGGGTGGTCCAGAGGGCGGTCAGGCTGCT
CAAGAAGGAGCTGGGGGACGAGGTGGTGGTCTTCACGGACGTGTGCCTCTGCCACTACACCTCCCACGGCCACTGCGGGG
TCCTAGCCAAGAGGTGTCGCGGCGACCTCTGCGAGTACGTGGTGGACAACGACAAGACCTTGGAGCTGTTAGCTAAGGTC
GCCCTCTCCCACGCCAAGGCGGGGGCGGACGTGGTGGCCCCCTCCTCGATGATGGACGGCATGGTGCTGGCCATAAGGGA
GGCCTTGGACAAGGAGGGCTTCACGGACGTGGCTATAATGAGCTACGCCGCCAAGTACGCTTCCGCCTTCTACGGCCCCT
TCCGCGAGGCCGCCAGCAACGCCCCCCAGCACGGGGACAGGAGGACCTACCAGATGGACCCCAGGAACGGGGAGGAGGCC
TTGAAGGAGGTTATGTTGGACGTTAAGGAGGGGGCGGACATCTTGATGGTGAAGCCCGCGCTCTCCTACTTGGACGTTAT
AAGGAGGGTGAGGGAGGCCTTCCCCCACTACCCCTTAGCAGCATACAACGTCAGCGGAGAGTACAGCATGGTTAAGGCGG
CGGCCGAGAAGGGGCTGGTGGACGAGAAGAAGATAACTTTGGAAATACTGCACTCAATAAAGAGGGCCGGCGCAAACATA
ATAATAACGTACCACGCCTTGGAGGCCTCGAGGTGGCTCAAGGAAGGGTTGGACCTAAAGATGTTCTAG

Upstream 100 bases:

>100_bases
ACTGGGTACGCCTCGCCCTCTAGGGACCACCTCAAGCCCTTCGCCCCCGGGAGTTACCGGATATAGTCCTCGGAGCTTTA
CTGCGGAGCGGAAGAGGGCC

Downstream 100 bases:

>100_bases
TTTTTAACCACGCCCCAGCCCGCCAGCCTCCAGCGGCCTTGTATCCTCCTGCTGAAGGCCACCCTCGCCCCCTTCCAGGC
CACCGCGGGCCTCTGGAGCT

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MSFPRLRPRRLRKNLYLRDLVAETRLSPRDFIYPIFVKEGIDKPEEISAMPGQMRWPVGEELVKHVQEALDLGVRSVLLF
GVPKHKDDLGSSAYDDQGVVQRAVRLLKKELGDEVVVFTDVCLCHYTSHGHCGVLAKRCRGDLCEYVVDNDKTLELLAKV
ALSHAKAGADVVAPSSMMDGMVLAIREALDKEGFTDVAIMSYAAKYASAFYGPFREAASNAPQHGDRRTYQMDPRNGEEA
LKEVMLDVKEGADILMVKPALSYLDVIRRVREAFPHYPLAAYNVSGEYSMVKAAAEKGLVDEKKITLEILHSIKRAGANI
IITYHALEASRWLKEGLDLKMF

Sequences:

>Translated_342_residues
MSFPRLRPRRLRKNLYLRDLVAETRLSPRDFIYPIFVKEGIDKPEEISAMPGQMRWPVGEELVKHVQEALDLGVRSVLLF
GVPKHKDDLGSSAYDDQGVVQRAVRLLKKELGDEVVVFTDVCLCHYTSHGHCGVLAKRCRGDLCEYVVDNDKTLELLAKV
ALSHAKAGADVVAPSSMMDGMVLAIREALDKEGFTDVAIMSYAAKYASAFYGPFREAASNAPQHGDRRTYQMDPRNGEEA
LKEVMLDVKEGADILMVKPALSYLDVIRRVREAFPHYPLAAYNVSGEYSMVKAAAEKGLVDEKKITLEILHSIKRAGANI
IITYHALEASRWLKEGLDLKMF
>Mature_341_residues
SFPRLRPRRLRKNLYLRDLVAETRLSPRDFIYPIFVKEGIDKPEEISAMPGQMRWPVGEELVKHVQEALDLGVRSVLLFG
VPKHKDDLGSSAYDDQGVVQRAVRLLKKELGDEVVVFTDVCLCHYTSHGHCGVLAKRCRGDLCEYVVDNDKTLELLAKVA
LSHAKAGADVVAPSSMMDGMVLAIREALDKEGFTDVAIMSYAAKYASAFYGPFREAASNAPQHGDRRTYQMDPRNGEEAL
KEVMLDVKEGADILMVKPALSYLDVIRRVREAFPHYPLAAYNVSGEYSMVKAAAEKGLVDEKKITLEILHSIKRAGANII
ITYHALEASRWLKEGLDLKMF

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=338, Percent_Identity=43.7869822485207, Blast_Score=266, Evalue=2e-71,
Organism=Escherichia coli, GI87081728, Length=322, Percent_Identity=52.4844720496894, Blast_Score=320, Evalue=8e-89,
Organism=Saccharomyces cerevisiae, GI6321398, Length=323, Percent_Identity=40.8668730650155, Blast_Score=233, Evalue=3e-62,
Organism=Drosophila melanogaster, GI21358291, Length=323, Percent_Identity=39.6284829721362, Blast_Score=238, Evalue=3e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 38297; Mature: 38166

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFPRLRPRRLRKNLYLRDLVAETRLSPRDFIYPIFVKEGIDKPEEISAMPGQMRWPVGE
CCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHEEHHHHHHCCCCCHHHHCCCCCCCCCHHH
ELVKHVQEALDLGVRSVLLFGVPKHKDDLGSSAYDDQGVVQRAVRLLKKELGDEVVVFTD
HHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHH
VCLCHYTSHGHCGVLAKRCRGDLCEYVVDNDKTLELLAKVALSHAKAGADVVAPSSMMDG
HHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
MVLAIREALDKEGFTDVAIMSYAAKYASAFYGPFREAASNAPQHGDRRTYQMDPRNGEEA
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCHHHH
LKEVMLDVKEGADILMVKPALSYLDVIRRVREAFPHYPLAAYNVSGEYSMVKAAAEKGLV
HHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCC
DEKKITLEILHSIKRAGANIIITYHALEASRWLKEGLDLKMF
CHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SFPRLRPRRLRKNLYLRDLVAETRLSPRDFIYPIFVKEGIDKPEEISAMPGQMRWPVGE
CCCCCCHHHHHHHHHHHHHHHHHCCCHHHHEEHHHHHHCCCCCHHHHCCCCCCCCCHHH
ELVKHVQEALDLGVRSVLLFGVPKHKDDLGSSAYDDQGVVQRAVRLLKKELGDEVVVFTD
HHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHH
VCLCHYTSHGHCGVLAKRCRGDLCEYVVDNDKTLELLAKVALSHAKAGADVVAPSSMMDG
HHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
MVLAIREALDKEGFTDVAIMSYAAKYASAFYGPFREAASNAPQHGDRRTYQMDPRNGEEA
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCHHHH
LKEVMLDVKEGADILMVKPALSYLDVIRRVREAFPHYPLAAYNVSGEYSMVKAAAEKGLV
HHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCC
DEKKITLEILHSIKRAGANIIITYHALEASRWLKEGLDLKMF
CHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1672867; 9384377 [H]