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The map label for this gene is pgk [H]

Identifier: 156937069

GI number: 156937069

Start: 235012

End: 236238

Strand: Reverse

Name: pgk [H]

Synonym: Igni_0274

Alternate gene names: 156937069

Gene position: 236238-235012 (Counterclockwise)

Preceding gene: 156937073

Following gene: 156937068

Centisome position: 18.21

GC content: 58.52

Gene sequence:

>1227_bases
TTGAAGTTGGAGGAGCTCCCCACCTTGGATGATATAGACGTTTCAAACAAAAAAGTATTGTTGAGGGTAGACTTCAACAG
CCCCGTAAAGGACGAGGAGCTCTTGGACGACTCGAGGATAAGGGCCCACGTGCCCACGATAAAGGAGCTCTTGGAAAAGG
GGGCAGCGGTCGTGGTGATCTCCCACCAAGGGCGCCCGGGGGAGGACGACTTCATCACCTTGGAGCCGCACGCCAAGAAG
CTCTCGGAGGTCCTCGGCCAGACCATAAACTTCGTGGACGACGTGATGGGCCCCGAGGCCCGGAGGAGGATAAAGGAGCT
GAAGCCCGGGGAAGTCCTCTTGCTCGACAACGTCAGGTTCTTAGCGGAAGAGAACATAAACGCTCCCCCGGAGGTACAAG
CGAAGACGTACCTCGTTAGGAGGCTGGCGAAGCTGTTCGACGTTTACGTAAACGACGCCTTCGCCACCGCCCACCGGTCC
CAGCCCAGCGTGGTGGGCTTCCCCTTGGTCCTCCCCTCAGCGATGGGAAGGGTGATGGAAAAGGAGGTAAAGGCGTTAAG
TAAGGTGTTCAACCCCCAAGTCTCCCCCAAGATATTCGTGCTGGGAGGGGGGAAGGTGCCGGACACCATAGTTATAATAG
AGAACTTAGTGAAGAACAAGGTTGCCGACAGGATACTGACTACCGGGCTTGTGGCGGAGGTATTCTTGGTCGCTAAAGGG
GTAGACTTGGGAGAGAAGAACATGGAAGTGCTGGAGAAGAAGGGACTCTTGGGGCTCATACCCAAGGCCAAGAAACTCTT
GCTGGCCGGGGCACCTATAGAGACTCCGTTGGACTTCAAGACCGTGGACGAGGAGGGGAACGTGAGGGTAGAGCCGGCCT
TCTCGGTGACGGGGCTCATAAGGGACGTGGGGCCGGCGACCGTCAAGACCTACGGGGAACTGATGAAAGACGCGAGGGTA
ATAGTCATGAGGGGTCCCGCCGGCGTCATGGAGGACCCGCGCTTCCGCGAGGGCACCCGGGAGCTGGTCAGGCTGGCGCT
TGAGAGCGGCGCCTTCACCATCTTCGGCGGGGGCCACTTAACTGCCGTGATCAAGGAGCTAGGTATGGAGGACAAGGTAG
GCCACATAAGCACCGGCGGGGGCGCGCTACTGAGCTTCCTAGCCGGCAAGGAGCTGCCGGCGCTCAAAGCCTTAGAGATG
TCCAAGGAGAGGTTTTGGAGCAAGTAG

Upstream 100 bases:

>100_bases
GAAGTGGGCAGCCCCTGAGCGAGTGATGTGCCTAGTCTCACTCCGACCCCCGCTTGCCCTATAACCAAGGGCCTCGCTAG
AGAGGTCGGGAAGACCCTCC

Downstream 100 bases:

>100_bases
CCTCGGCCGAGAACCGAGTCCTCACCCCTCGGCGCCCATGACAGCTCCTCACCGGCCCCCTACAGAAAACCGAGGGAGGT
ATTGAGGTTGTTAGCGATTA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MKLEELPTLDDIDVSNKKVLLRVDFNSPVKDEELLDDSRIRAHVPTIKELLEKGAAVVVISHQGRPGEDDFITLEPHAKK
LSEVLGQTINFVDDVMGPEARRRIKELKPGEVLLLDNVRFLAEENINAPPEVQAKTYLVRRLAKLFDVYVNDAFATAHRS
QPSVVGFPLVLPSAMGRVMEKEVKALSKVFNPQVSPKIFVLGGGKVPDTIVIIENLVKNKVADRILTTGLVAEVFLVAKG
VDLGEKNMEVLEKKGLLGLIPKAKKLLLAGAPIETPLDFKTVDEEGNVRVEPAFSVTGLIRDVGPATVKTYGELMKDARV
IVMRGPAGVMEDPRFREGTRELVRLALESGAFTIFGGGHLTAVIKELGMEDKVGHISTGGGALLSFLAGKELPALKALEM
SKERFWSK

Sequences:

>Translated_408_residues
MKLEELPTLDDIDVSNKKVLLRVDFNSPVKDEELLDDSRIRAHVPTIKELLEKGAAVVVISHQGRPGEDDFITLEPHAKK
LSEVLGQTINFVDDVMGPEARRRIKELKPGEVLLLDNVRFLAEENINAPPEVQAKTYLVRRLAKLFDVYVNDAFATAHRS
QPSVVGFPLVLPSAMGRVMEKEVKALSKVFNPQVSPKIFVLGGGKVPDTIVIIENLVKNKVADRILTTGLVAEVFLVAKG
VDLGEKNMEVLEKKGLLGLIPKAKKLLLAGAPIETPLDFKTVDEEGNVRVEPAFSVTGLIRDVGPATVKTYGELMKDARV
IVMRGPAGVMEDPRFREGTRELVRLALESGAFTIFGGGHLTAVIKELGMEDKVGHISTGGGALLSFLAGKELPALKALEM
SKERFWSK
>Mature_408_residues
MKLEELPTLDDIDVSNKKVLLRVDFNSPVKDEELLDDSRIRAHVPTIKELLEKGAAVVVISHQGRPGEDDFITLEPHAKK
LSEVLGQTINFVDDVMGPEARRRIKELKPGEVLLLDNVRFLAEENINAPPEVQAKTYLVRRLAKLFDVYVNDAFATAHRS
QPSVVGFPLVLPSAMGRVMEKEVKALSKVFNPQVSPKIFVLGGGKVPDTIVIIENLVKNKVADRILTTGLVAEVFLVAKG
VDLGEKNMEVLEKKGLLGLIPKAKKLLLAGAPIETPLDFKTVDEEGNVRVEPAFSVTGLIRDVGPATVKTYGELMKDARV
IVMRGPAGVMEDPRFREGTRELVRLALESGAFTIFGGGHLTAVIKELGMEDKVGHISTGGGALLSFLAGKELPALKALEM
SKERFWSK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=423, Percent_Identity=37.8250591016548, Blast_Score=232, Evalue=4e-61,
Organism=Homo sapiens, GI31543397, Length=423, Percent_Identity=37.3522458628842, Blast_Score=232, Evalue=6e-61,
Organism=Escherichia coli, GI1789294, Length=400, Percent_Identity=34.75, Blast_Score=214, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI17508823, Length=412, Percent_Identity=37.621359223301, Blast_Score=229, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6319857, Length=425, Percent_Identity=36.2352941176471, Blast_Score=211, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24581203, Length=417, Percent_Identity=35.0119904076739, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI17136394, Length=419, Percent_Identity=36.9928400954654, Blast_Score=213, Evalue=2e-55,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 44630; Mature: 44630

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLEELPTLDDIDVSNKKVLLRVDFNSPVKDEELLDDSRIRAHVPTIKELLEKGAAVVVI
CCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHCCCHHHEECHHHHHHHHCCCEEEEE
SHQGRPGEDDFITLEPHAKKLSEVLGQTINFVDDVMGPEARRRIKELKPGEVLLLDNVRF
ECCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEECCCHH
LAEENINAPPEVQAKTYLVRRLAKLFDVYVNDAFATAHRSQPSVVGFPLVLPSAMGRVME
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHH
KEVKALSKVFNPQVSPKIFVLGGGKVPDTIVIIENLVKNKVADRILTTGLVAEVFLVAKG
HHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VDLGEKNMEVLEKKGLLGLIPKAKKLLLAGAPIETPLDFKTVDEEGNVRVEPAFSVTGLI
CCCCHHHHHHHHHCCCEEECCHHHHHEEECCCCCCCCCCEEECCCCCEEEECCHHHHHHH
RDVGPATVKTYGELMKDARVIVMRGPAGVMEDPRFREGTRELVRLALESGAFTIFGGGHL
HHCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECHHH
TAVIKELGMEDKVGHISTGGGALLSFLAGKELPALKALEMSKERFWSK
HHHHHHCCCCCCCCEEECCHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKLEELPTLDDIDVSNKKVLLRVDFNSPVKDEELLDDSRIRAHVPTIKELLEKGAAVVVI
CCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHCCCHHHEECHHHHHHHHCCCEEEEE
SHQGRPGEDDFITLEPHAKKLSEVLGQTINFVDDVMGPEARRRIKELKPGEVLLLDNVRF
ECCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEECCCHH
LAEENINAPPEVQAKTYLVRRLAKLFDVYVNDAFATAHRSQPSVVGFPLVLPSAMGRVME
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHH
KEVKALSKVFNPQVSPKIFVLGGGKVPDTIVIIENLVKNKVADRILTTGLVAEVFLVAKG
HHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VDLGEKNMEVLEKKGLLGLIPKAKKLLLAGAPIETPLDFKTVDEEGNVRVEPAFSVTGLI
CCCCHHHHHHHHHCCCEEECCHHHHHEEECCCCCCCCCCEEECCCCCEEEECCHHHHHHH
RDVGPATVKTYGELMKDARVIVMRGPAGVMEDPRFREGTRELVRLALESGAFTIFGGGHL
HHCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECHHH
TAVIKELGMEDKVGHISTGGGALLSFLAGKELPALKALEMSKERFWSK
HHHHHHCCCCCCCCEEECCHHHHHHHHHCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8521845; 10701121; 11427726 [H]