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The map label for this gene is braD [H]

Identifier: 154248136

GI number: 154248136

Start: 4669006

End: 4670898

Strand: Direct

Name: braD [H]

Synonym: Xaut_4216

Alternate gene names: 154248136

Gene position: 4669006-4670898 (Clockwise)

Preceding gene: 154248135

Following gene: 154248137

Centisome position: 87.95

GC content: 69.04

Gene sequence:

>1893_bases
GTGGAATTCCTGTTGGTCCAGGCCCTGAGCGGGCTCGCCAGCGCCGCGTCGCTGTTTCTGGTGGCGTCGGGCCTGTCGAT
CATCTTCGGCGTGACGCGCATTGTGAATTTCGCGCACGGCGCCTTCTACATGCTGGGCGCCTACGTGGCCTATGTGCTCA
CCACGCGCTTTGCCGGCCCCATCGGCTTCTGGGGCGCGATCCTCGCTGCCTCGGTCATCGTCGCGGCGCTGGGCGCGCTG
ATGGAAATGGTGCTGCTGCGCCGCATCTACCACGCTCCCGAACTGTTCCAGCTCCTCGCCACCTTCGGCGTGACGCTCAT
GGTGCAGGACCTGGTGGTGGTGATCTGGGGTCCCGAGGACCTGCTCGGCCCGCGCGCCCCCGGCCTTGCCGGCGCGGTCA
GCCTGTTCGGCCGCATGGTGCCCACCTACGATCTGTTCCTGATGGTGCTTGGCCCGGTGGTGCTCGGCGCGCTGTGGTTG
TTGTTCCACCAGACCCGCTGGGGCGTTCTGGTGCGCGCAGCGACGCAGGACCGCGAGATGGTGGCGGCCCTCGGCGTGAA
CCAGAAATGGCTCTTCACCGGTGTGTTCGCCCTCGGCGTCTTCCTCGCCGCGCTGGGCGGCGCCCTGCAGATCCCGCGCA
CCACGGTGACCCACACCATGGACCTCTCCGTCATCGTCGAGGTGTTCGTGGTGGTGGTCATCGGCGGGCTCGGCTCGGTG
CTGGGGGCGTTCGTCGCCGCCATCATCGTGGCGGAGCTGAACGCCTTCGGCATTCTCATCCTGCCGCAGATCTCGCTCAT
CATGGTGTTCCTGGTGATGGCGGTGGTGCTGGTGGTGCGGCCCTGGGGCCTGTTCGGCAAGGCCGAGAGCGCGCCGCGCC
CCGCCGCCGGCCTCACCATCCGGCCGTGGCGGCCGTTCTCCACCGCCGAGCGGGTGGCGGTGGCCGTGGCGGTGGCGGCC
GTGGCGACGCTGCCCTTCTTCCTCGGCAACTACGCCCTCACCGTCGGCGCGGAAATCCTCATCTTCGTGCTGTTCGCCGC
CAGCCTGCATTTCATCATGACCGTGGGCGGCCTCGCCTCCTTCGGCCATGCCGCCTATTTCGGCCTCGGCGCGTATGGCG
CGGCCTTCATGGTGAAGTTCCTCGGCGCGCCCATGGAGGCGGCCCTCATCGTCGGCCCGGCCCTTGGGCTGGTGGGGGCG
GTGCTGTTCGGCTGGTTCTGCGTGCGGCTCTCGGGCGTCTATTTCGCCATGCTGACGCTGGCCTTCGCGCAGATCGCCTG
GTCCGTGGCCTTCCAGTGGGTGGACGTGACCGGCGGCGATAACGGCCTGCTGGGCATCTGGCCCTCGGCGTGGGCGGCCA
AGCCCCAGGGCTTCTTTTGGCTGGCGCTGGCGGTGGCGGTGATCGGCGTCGGCCTGTTGCGGATGCTGGTGTTCTCGCCC
TTCGGCTACGGCCTGCGGGCGGTGCGCGACAGCACCCTGCGCGCAGAGGCCATCGGGCTGGATCGGGCGCGGCTACAGTG
GGCCGCCTTCGTGGTCGCCGGCACCTTCGCCGGCATCGCCGGTGCGCTGTTTGCCTTCCTGAAGGGCTCGGTGTTCCCGG
ACTTCATGGGCATCCCGACCTCGGTGGACGGCCTTGTGATGGTGTTGCTCGGCGGCATCGAGACGGTGTCCGGCGCGGTG
GTGGGGGCGGTGCTCTACAAGGCGCTCGCCATCTGGCTCATGAGCCAGACCGATCTCTCCAAGCTGGTGCTGGGGGCGGT
GATCGTGGTGGTGGTGCTGGCCTTCCCGAAGGGGGTGGTGGGCTTCATCGAGGACATGCGCCACCGCTGGTTCGCTCCAA
AGAAACCCACGGCCGAGGCGGCGGCCCTCGCCAAGGTGGAGGCCGCGCGATGA

Upstream 100 bases:

>100_bases
AGCCAGGGCGCCCGCGCCCCGGCGCAAGACCCACCTCCCTCGACGGCGGCGGGTCCAAGACCAAAATCCTTTCCAGGCCG
GTGAACGAGAGGCGAGACAA

Downstream 100 bases:

>100_bases
GCTTGCTGCTTCAGGTCGAGAACCTCGAAAAATCCTATGGCGGCGTCCATGCGGTGCGCGGCGTCACCTTCAGCCTGGAG
GCCGGCACGCTTTTGGCCCT

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 630; Mature: 630

Protein sequence:

>630_residues
MEFLLVQALSGLASAASLFLVASGLSIIFGVTRIVNFAHGAFYMLGAYVAYVLTTRFAGPIGFWGAILAASVIVAALGAL
MEMVLLRRIYHAPELFQLLATFGVTLMVQDLVVVIWGPEDLLGPRAPGLAGAVSLFGRMVPTYDLFLMVLGPVVLGALWL
LFHQTRWGVLVRAATQDREMVAALGVNQKWLFTGVFALGVFLAALGGALQIPRTTVTHTMDLSVIVEVFVVVVIGGLGSV
LGAFVAAIIVAELNAFGILILPQISLIMVFLVMAVVLVVRPWGLFGKAESAPRPAAGLTIRPWRPFSTAERVAVAVAVAA
VATLPFFLGNYALTVGAEILIFVLFAASLHFIMTVGGLASFGHAAYFGLGAYGAAFMVKFLGAPMEAALIVGPALGLVGA
VLFGWFCVRLSGVYFAMLTLAFAQIAWSVAFQWVDVTGGDNGLLGIWPSAWAAKPQGFFWLALAVAVIGVGLLRMLVFSP
FGYGLRAVRDSTLRAEAIGLDRARLQWAAFVVAGTFAGIAGALFAFLKGSVFPDFMGIPTSVDGLVMVLLGGIETVSGAV
VGAVLYKALAIWLMSQTDLSKLVLGAVIVVVVLAFPKGVVGFIEDMRHRWFAPKKPTAEAAALAKVEAAR

Sequences:

>Translated_630_residues
MEFLLVQALSGLASAASLFLVASGLSIIFGVTRIVNFAHGAFYMLGAYVAYVLTTRFAGPIGFWGAILAASVIVAALGAL
MEMVLLRRIYHAPELFQLLATFGVTLMVQDLVVVIWGPEDLLGPRAPGLAGAVSLFGRMVPTYDLFLMVLGPVVLGALWL
LFHQTRWGVLVRAATQDREMVAALGVNQKWLFTGVFALGVFLAALGGALQIPRTTVTHTMDLSVIVEVFVVVVIGGLGSV
LGAFVAAIIVAELNAFGILILPQISLIMVFLVMAVVLVVRPWGLFGKAESAPRPAAGLTIRPWRPFSTAERVAVAVAVAA
VATLPFFLGNYALTVGAEILIFVLFAASLHFIMTVGGLASFGHAAYFGLGAYGAAFMVKFLGAPMEAALIVGPALGLVGA
VLFGWFCVRLSGVYFAMLTLAFAQIAWSVAFQWVDVTGGDNGLLGIWPSAWAAKPQGFFWLALAVAVIGVGLLRMLVFSP
FGYGLRAVRDSTLRAEAIGLDRARLQWAAFVVAGTFAGIAGALFAFLKGSVFPDFMGIPTSVDGLVMVLLGGIETVSGAV
VGAVLYKALAIWLMSQTDLSKLVLGAVIVVVVLAFPKGVVGFIEDMRHRWFAPKKPTAEAAALAKVEAAR
>Mature_630_residues
MEFLLVQALSGLASAASLFLVASGLSIIFGVTRIVNFAHGAFYMLGAYVAYVLTTRFAGPIGFWGAILAASVIVAALGAL
MEMVLLRRIYHAPELFQLLATFGVTLMVQDLVVVIWGPEDLLGPRAPGLAGAVSLFGRMVPTYDLFLMVLGPVVLGALWL
LFHQTRWGVLVRAATQDREMVAALGVNQKWLFTGVFALGVFLAALGGALQIPRTTVTHTMDLSVIVEVFVVVVIGGLGSV
LGAFVAAIIVAELNAFGILILPQISLIMVFLVMAVVLVVRPWGLFGKAESAPRPAAGLTIRPWRPFSTAERVAVAVAVAA
VATLPFFLGNYALTVGAEILIFVLFAASLHFIMTVGGLASFGHAAYFGLGAYGAAFMVKFLGAPMEAALIVGPALGLVGA
VLFGWFCVRLSGVYFAMLTLAFAQIAWSVAFQWVDVTGGDNGLLGIWPSAWAAKPQGFFWLALAVAVIGVGLLRMLVFSP
FGYGLRAVRDSTLRAEAIGLDRARLQWAAFVVAGTFAGIAGALFAFLKGSVFPDFMGIPTSVDGLVMVLLGGIETVSGAV
VGAVLYKALAIWLMSQTDLSKLVLGAVIVVVVLAFPKGVVGFIEDMRHRWFAPKKPTAEAAALAKVEAAR

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=303, Percent_Identity=24.0924092409241, Blast_Score=83, Evalue=6e-17,
Organism=Escherichia coli, GI1789866, Length=302, Percent_Identity=25.8278145695364, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 66456; Mature: 66456

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFLLVQALSGLASAASLFLVASGLSIIFGVTRIVNFAHGAFYMLGAYVAYVLTTRFAGP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IGFWGAILAASVIVAALGALMEMVLLRRIYHAPELFQLLATFGVTLMVQDLVVVIWGPED
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEEECCHH
LLGPRAPGLAGAVSLFGRMVPTYDLFLMVLGPVVLGALWLLFHQTRWGVLVRAATQDREM
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCHHH
VAALGVNQKWLFTGVFALGVFLAALGGALQIPRTTVTHTMDLSVIVEVFVVVVIGGLGSV
HHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGAFVAAIIVAELNAFGILILPQISLIMVFLVMAVVLVVRPWGLFGKAESAPRPAAGLTI
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEE
RPWRPFSTAERVAVAVAVAAVATLPFFLGNYALTVGAEILIFVLFAASLHFIMTVGGLAS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGHAAYFGLGAYGAAFMVKFLGAPMEAALIVGPALGLVGAVLFGWFCVRLSGVYFAMLTL
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFAQIAWSVAFQWVDVTGGDNGLLGIWPSAWAAKPQGFFWLALAVAVIGVGLLRMLVFSP
HHHHHHHHHHHHEEEEECCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
FGYGLRAVRDSTLRAEAIGLDRARLQWAAFVVAGTFAGIAGALFAFLKGSVFPDFMGIPT
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
SVDGLVMVLLGGIETVSGAVVGAVLYKALAIWLMSQTDLSKLVLGAVIVVVVLAFPKGVV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHH
GFIEDMRHRWFAPKKPTAEAAALAKVEAAR
HHHHHHHHHCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MEFLLVQALSGLASAASLFLVASGLSIIFGVTRIVNFAHGAFYMLGAYVAYVLTTRFAGP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IGFWGAILAASVIVAALGALMEMVLLRRIYHAPELFQLLATFGVTLMVQDLVVVIWGPED
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEEECCHH
LLGPRAPGLAGAVSLFGRMVPTYDLFLMVLGPVVLGALWLLFHQTRWGVLVRAATQDREM
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCHHH
VAALGVNQKWLFTGVFALGVFLAALGGALQIPRTTVTHTMDLSVIVEVFVVVVIGGLGSV
HHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGAFVAAIIVAELNAFGILILPQISLIMVFLVMAVVLVVRPWGLFGKAESAPRPAAGLTI
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEE
RPWRPFSTAERVAVAVAVAAVATLPFFLGNYALTVGAEILIFVLFAASLHFIMTVGGLAS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGHAAYFGLGAYGAAFMVKFLGAPMEAALIVGPALGLVGAVLFGWFCVRLSGVYFAMLTL
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFAQIAWSVAFQWVDVTGGDNGLLGIWPSAWAAKPQGFFWLALAVAVIGVGLLRMLVFSP
HHHHHHHHHHHHEEEEECCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
FGYGLRAVRDSTLRAEAIGLDRARLQWAAFVVAGTFAGIAGALFAFLKGSVFPDFMGIPT
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
SVDGLVMVLLGGIETVSGAVVGAVLYKALAIWLMSQTDLSKLVLGAVIVVVVLAFPKGVV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHH
GFIEDMRHRWFAPKKPTAEAAALAKVEAAR
HHHHHHHHHCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]