Click here to switch to the map view.

The map label for this gene is cspE [H]

Identifier: 154248094

GI number: 154248094

Start: 4621513

End: 4622091

Strand: Direct

Name: cspE [H]

Synonym: Xaut_4174

Alternate gene names: 154248094

Gene position: 4621513-4622091 (Clockwise)

Preceding gene: 154248093

Following gene: 154248095

Centisome position: 87.05

GC content: 69.26

Gene sequence:

>579_bases
ATGGGGTCTGACGCACTGGGGCTGGGGCCACGGACCGCCGGCTCGTCGGAGGACGGACTTGAGGAGCCCGCCGGGCTCGT
CGAGGTTACCGGCTCCATCAAATGGTTCGATGCCTCCAAGGGCTACGGCTTCATCGTCCCGGACGACGGCGGCCCCGATG
TGCTGGTCCATGTCACCTGCCTGCGGCGCGACGGCTTCCAGACCGCCATGGAAGGCGCCCGCGTGGTCTGCGAGGCAGTC
CAGCGGGCCAAGGGGCGGCAGGCCTTCCGGGTGCTCTCCCTCGACCTCTCGACCGCGGTGCATACTGCTCCGCCGCAGCC
GCGCACCCGCACGGTGGTGGAACCCAGGAGCGGTTTCGAGCCGGCCACGGTGAAATGGTTCAACCGGCTGCGCGGCTTCG
GCTTCCTCACCCAGGGGGACGGCACGCCGGACATCTTCGTCCATATGGAGACGCTGCGCCGCCACGGCCTCACCGAACTT
CGGCCCGGCCAGACGGTGATGGTGCGCTACGGCGACGGCGATAAGGGCCTCATGGCCGCCGAAGTGAAGGCCGATACCGG
ACCCAGCGGGAACACCTGA

Upstream 100 bases:

>100_bases
GAAGAGAGGGTGCGAAGAGGGTGGGCGAAGCCCCGGCGAGTTGCTATCCTGAAACCGGCGCGGCAAAGCGTGCGCGGCCG
CGTGTAAGGTCGGGGTGGTC

Downstream 100 bases:

>100_bases
TCATGCCATTTGCCATCGTGAAGACCGGCGCTTCCCCCGCGCCGACGGGTCGCGGAATTTTGGGCCGCGCGGGTCGCGGC
AGAACCATCCCTGCTGGAAT

Product: cold-shock DNA-binding domain-containing protein

Products: NA

Alternate protein names: CSP-E [H]

Number of amino acids: Translated: 192; Mature: 191

Protein sequence:

>192_residues
MGSDALGLGPRTAGSSEDGLEEPAGLVEVTGSIKWFDASKGYGFIVPDDGGPDVLVHVTCLRRDGFQTAMEGARVVCEAV
QRAKGRQAFRVLSLDLSTAVHTAPPQPRTRTVVEPRSGFEPATVKWFNRLRGFGFLTQGDGTPDIFVHMETLRRHGLTEL
RPGQTVMVRYGDGDKGLMAAEVKADTGPSGNT

Sequences:

>Translated_192_residues
MGSDALGLGPRTAGSSEDGLEEPAGLVEVTGSIKWFDASKGYGFIVPDDGGPDVLVHVTCLRRDGFQTAMEGARVVCEAV
QRAKGRQAFRVLSLDLSTAVHTAPPQPRTRTVVEPRSGFEPATVKWFNRLRGFGFLTQGDGTPDIFVHMETLRRHGLTEL
RPGQTVMVRYGDGDKGLMAAEVKADTGPSGNT
>Mature_191_residues
GSDALGLGPRTAGSSEDGLEEPAGLVEVTGSIKWFDASKGYGFIVPDDGGPDVLVHVTCLRRDGFQTAMEGARVVCEAVQ
RAKGRQAFRVLSLDLSTAVHTAPPQPRTRTVVEPRSGFEPATVKWFNRLRGFGFLTQGDGTPDIFVHMETLRRHGLTELR
PGQTVMVRYGDGDKGLMAAEVKADTGPSGNT

Specific function: Unknown

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1786841, Length=69, Percent_Identity=44.9275362318841, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1789979, Length=67, Percent_Identity=46.2686567164179, Blast_Score=68, Evalue=4e-13,
Organism=Escherichia coli, GI2367114, Length=67, Percent_Identity=43.2835820895522, Blast_Score=64, Evalue=5e-12,
Organism=Escherichia coli, GI1787839, Length=68, Percent_Identity=42.6470588235294, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI1788126, Length=69, Percent_Identity=40.5797101449275, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1787107, Length=70, Percent_Identity=35.7142857142857, Blast_Score=60, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 20571; Mature: 20440

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSDALGLGPRTAGSSEDGLEEPAGLVEVTGSIKWFDASKGYGFIVPDDGGPDVLVHVTC
CCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCCEEEEEECCCCCCEEEEEEE
LRRDGFQTAMEGARVVCEAVQRAKGRQAFRVLSLDLSTAVHTAPPQPRTRTVVEPRSGFE
ECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEHHHHEECCCCCCCCCEEECCCCCCC
PATVKWFNRLRGFGFLTQGDGTPDIFVHMETLRRHGLTELRPGQTVMVRYGDGDKGLMAA
CHHHHHHHHHCCCEEEECCCCCCEEEEEEHHHHHCCCCCCCCCCEEEEEECCCCCCEEEE
EVKADTGPSGNT
EEECCCCCCCCC
>Mature Secondary Structure 
GSDALGLGPRTAGSSEDGLEEPAGLVEVTGSIKWFDASKGYGFIVPDDGGPDVLVHVTC
CCCCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCCEEEEEECCCCCCEEEEEEE
LRRDGFQTAMEGARVVCEAVQRAKGRQAFRVLSLDLSTAVHTAPPQPRTRTVVEPRSGFE
ECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEHHHHEECCCCCCCCCEEECCCCCCC
PATVKWFNRLRGFGFLTQGDGTPDIFVHMETLRRHGLTELRPGQTVMVRYGDGDKGLMAA
CHHHHHHHHHCCCEEEECCCCCCEEEEEEHHHHHCCCCCCCCCCEEEEEECCCCCCEEEE
EVKADTGPSGNT
EEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10993077 [H]