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The map label for this gene is recA

Identifier: 154248001

GI number: 154248001

Start: 4515218

End: 4516303

Strand: Direct

Name: recA

Synonym: Xaut_4080

Alternate gene names: 154248001

Gene position: 4515218-4516303 (Clockwise)

Preceding gene: 154247986

Following gene: 154248002

Centisome position: 85.05

GC content: 65.65

Gene sequence:

>1086_bases
ATGACTCAGTCGACACTCAGACTCGTCGAAGGTTCTTCCATGGATAAGACCAAGGCGCTGGATGCCGCTCTCTCCCAGAT
CGAGCGGCACTTCGGCAAGGGCTCGATCATGCGGCTCGGCAAGGGCGGCAAAGCCTTGGAAATCGAGAGCGTTCCCACCG
GCTCGCTCGGGCTGGACATCGCCATCGGCATCGGCGGCCTGCCCAAGGGCCGCGTCGTGGAGATCTTCGGGCCGGAATCC
TCCGGCAAGACCACCCTGGCGCTGCACACGGTCGCGGAAGCGCAGAAGCGGGGCGGCACCTGCGCCTTCATCGATGCCGA
GCACGCGCTCGACCCCATCTATGCGCGCAAGCTGGGCGTCAATCTCGACGACCTGCTCATCTCCCAGCCCGACGCGGGCG
AGCAGGCGCTGGAAATCGCCGACACGCTGGTGCGCTCGGGCGCCATCGACGTGCTGGTGGTCGATTCGGTGGCCGCGCTC
ACCCCGCGCGCCGAACTGGATGGCGAGATGGGCGACAGCCAGCCCGGCATGCAGGCGCGCCTCATGAGCCAGGCGCTGCG
CAAGCTCACGGCCTCCATCTCCAAGTCCAACACCATGGTCATCTTCATCAACCAGATCCGGATGAAGATCGGCGTCATGT
ACGGCTCGCCGGAGACCACCACCGGCGGCAATGCGCTCAAGTTCTACGCCTCGGTGCGGCTCGATATCCGCCGTATCGGC
ATGATCAAGGAGCGCGACGAGGTGGTGGGCAACCAGACCCGCGTGAAGGTGGTGAAGAACAAGCTGGCGCCGCCCTTCAA
GCAGGTGGAATTCGACATCATGTACGGCGAGGGGATTTCCAAGACCGGCGAACTGCTGGATCTCGGGGTCAAGGCCGGCG
TGGTGGAGAAGTCCGGCGCCTGGTTCTCCCACGACAGCCAGCGCCTCGGCCAGGGCCGCGAGAACGCCAAGACCTTCCTG
AAGCAGAATCAGGAGATTGCCGGCCGCATCGAGGCGGCGATCCGCCAGAATGCGGGCCTCATCGCCGAGAAGATCCTCGC
CGGCACGCCGGAGGATGACGCCGACGGCGACCCGGCTGACGCCTGA

Upstream 100 bases:

>100_bases
TGTCTGGACGGCCCAGATGAGAACATATTGCGAACTGAGAACAAAGCATGTACATGTGCAGTGTTCTCATTTCCCCTTGC
ACCAGCACCGGAGCGCGGCG

Downstream 100 bases:

>100_bases
TGGATCGCTGAGGCGTTTCTGGACAAGCGGCGCACCCATCGCTAAACGAACCCGGTTCCGGCGGGCGTCATCGCGCCTTC
GCCGGAACCGCGCGGCGGGC

Product: recombinase A

Products: NA

Alternate protein names: Recombinase A [H]

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MTQSTLRLVEGSSMDKTKALDAALSQIERHFGKGSIMRLGKGGKALEIESVPTGSLGLDIAIGIGGLPKGRVVEIFGPES
SGKTTLALHTVAEAQKRGGTCAFIDAEHALDPIYARKLGVNLDDLLISQPDAGEQALEIADTLVRSGAIDVLVVDSVAAL
TPRAELDGEMGDSQPGMQARLMSQALRKLTASISKSNTMVIFINQIRMKIGVMYGSPETTTGGNALKFYASVRLDIRRIG
MIKERDEVVGNQTRVKVVKNKLAPPFKQVEFDIMYGEGISKTGELLDLGVKAGVVEKSGAWFSHDSQRLGQGRENAKTFL
KQNQEIAGRIEAAIRQNAGLIAEKILAGTPEDDADGDPADA

Sequences:

>Translated_361_residues
MTQSTLRLVEGSSMDKTKALDAALSQIERHFGKGSIMRLGKGGKALEIESVPTGSLGLDIAIGIGGLPKGRVVEIFGPES
SGKTTLALHTVAEAQKRGGTCAFIDAEHALDPIYARKLGVNLDDLLISQPDAGEQALEIADTLVRSGAIDVLVVDSVAAL
TPRAELDGEMGDSQPGMQARLMSQALRKLTASISKSNTMVIFINQIRMKIGVMYGSPETTTGGNALKFYASVRLDIRRIG
MIKERDEVVGNQTRVKVVKNKLAPPFKQVEFDIMYGEGISKTGELLDLGVKAGVVEKSGAWFSHDSQRLGQGRENAKTFL
KQNQEIAGRIEAAIRQNAGLIAEKILAGTPEDDADGDPADA
>Mature_360_residues
TQSTLRLVEGSSMDKTKALDAALSQIERHFGKGSIMRLGKGGKALEIESVPTGSLGLDIAIGIGGLPKGRVVEIFGPESS
GKTTLALHTVAEAQKRGGTCAFIDAEHALDPIYARKLGVNLDDLLISQPDAGEQALEIADTLVRSGAIDVLVVDSVAALT
PRAELDGEMGDSQPGMQARLMSQALRKLTASISKSNTMVIFINQIRMKIGVMYGSPETTTGGNALKFYASVRLDIRRIGM
IKERDEVVGNQTRVKVVKNKLAPPFKQVEFDIMYGEGISKTGELLDLGVKAGVVEKSGAWFSHDSQRLGQGRENAKTFLK
QNQEIAGRIEAAIRQNAGLIAEKILAGTPEDDADGDPADA

Specific function: Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family [H]

Homologues:

Organism=Escherichia coli, GI1789051, Length=322, Percent_Identity=68.0124223602485, Blast_Score=464, Evalue=1e-132,

Paralogues:

None

Copy number: 1548 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800-1200 (L-broth) 40,000-60,000 (L-broth + Nalidixate) 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013765
- InterPro:   IPR020584
- InterPro:   IPR020588
- InterPro:   IPR020587 [H]

Pfam domain/function: PF00154 RecA [H]

EC number: NA

Molecular weight: Translated: 38484; Mature: 38352

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00321 RECA_1 ; PS50162 RECA_2 ; PS50163 RECA_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQSTLRLVEGSSMDKTKALDAALSQIERHFGKGSIMRLGKGGKALEIESVPTGSLGLDI
CCCHHEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCCCCCCCCEEE
AIGIGGLPKGRVVEIFGPESSGKTTLALHTVAEAQKRGGTCAFIDAEHALDPIYARKLGV
EEECCCCCCCCEEEEECCCCCCCEEEEEHHHHHHHHCCCEEEEEEHHHHCCHHHHHHHCC
NLDDLLISQPDAGEQALEIADTLVRSGAIDVLVVDSVAALTPRAELDGEMGDSQPGMQAR
CHHHHEECCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHCCCHHCCCCCCCCCCCHHHH
LMSQALRKLTASISKSNTMVIFINQIRMKIGVMYGSPETTTGGNALKFYASVRLDIRRIG
HHHHHHHHHHHHHCCCCEEEEEEEEEEEEEEEEECCCCCCCCCCEEEEEEEEEHHHHHHC
MIKERDEVVGNQTRVKVVKNKLAPPFKQVEFDIMYGEGISKTGELLDLGVKAGVVEKSGA
CHHHHHHHHCCCHHHHHHHHHCCCCHHHEEEEEEECCCCCCCCHHHHHCCCCCEEECCCC
WFSHDSQRLGQGRENAKTFLKQNQEIAGRIEAAIRQNAGLIAEKILAGTPEDDADGDPAD
CCCCCHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCC
A
C
>Mature Secondary Structure 
TQSTLRLVEGSSMDKTKALDAALSQIERHFGKGSIMRLGKGGKALEIESVPTGSLGLDI
CCHHEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCCCCCCCCEEE
AIGIGGLPKGRVVEIFGPESSGKTTLALHTVAEAQKRGGTCAFIDAEHALDPIYARKLGV
EEECCCCCCCCEEEEECCCCCCCEEEEEHHHHHHHHCCCEEEEEEHHHHCCHHHHHHHCC
NLDDLLISQPDAGEQALEIADTLVRSGAIDVLVVDSVAALTPRAELDGEMGDSQPGMQAR
CHHHHEECCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHCCCHHCCCCCCCCCCCHHHH
LMSQALRKLTASISKSNTMVIFINQIRMKIGVMYGSPETTTGGNALKFYASVRLDIRRIG
HHHHHHHHHHHHHCCCCEEEEEEEEEEEEEEEEECCCCCCCCCCEEEEEEEEEHHHHHHC
MIKERDEVVGNQTRVKVVKNKLAPPFKQVEFDIMYGEGISKTGELLDLGVKAGVVEKSGA
CHHHHHHHHCCCHHHHHHHHHCCCCHHHEEEEEEECCCCCCCCHHHHHCCCCCEEECCCC
WFSHDSQRLGQGRENAKTFLKQNQEIAGRIEAAIRQNAGLIAEKILAGTPEDDADGDPAD
CCCCCHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]