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The map label for this gene is braC [H]

Identifier: 154247974

GI number: 154247974

Start: 4484531

End: 4485751

Strand: Direct

Name: braC [H]

Synonym: Xaut_4053

Alternate gene names: 154247974

Gene position: 4484531-4485751 (Clockwise)

Preceding gene: 154247967

Following gene: 154247975

Centisome position: 84.47

GC content: 65.52

Gene sequence:

>1221_bases
ATGCGAGTGAGCGGAATGATTGCGGCTGTCGCCGCAAGCATCGCTATGGCTGGCGTCTCCATCGCAGGCGTCGCCGTGGC
CGCGGAACCCATCAAGATTGGCCTCAGCGGCCCCTTCACCGGCGGTTCGTCCTCCATGGGCGTGAGCATGCGCGACGGCG
TGAAGCTCGCGGTGACGGAAATCAACAAGAACGGCGGCGTGCTCGGCCGCCAGCTGGTGCTGGTCGAGCGTGACGACGAG
GCCAAGAACGAGGTGGGCGTGCAGATCGCCCAGGAGCTCATCAACAAGGAAGGGGTGGTCGCCACCCTCGGCTATATCAA
TACCGGCGTGGCCCTCGCCTCGCAGCGCTTCTACCAGGAAGCGGAAATCCCGGTGATCAACAACGTGGCCACCGGCACGC
TGATTTCCAAGCAGTTCCTGCCGCCGGACAACAAGTCCAACTACATCTTCCGCACCTCCGCCAACGACGCGATCCAGAGC
GTCATGATCGCGGATGAGGCGGTGAAGCGGCAGGGCTTCAAGAAGCCGGCCATCCTCGCCGACTCGACCAATTACGGCCA
GCTCGGCCGCGCCGACCTCGAGAAGGCGCTGGCGGTCCTGAACGTGAAGCCGGTGGTGACCGAGAAGTTCAACATCGGCG
ACACCGACATGACCGCGCAGCTGCTGCGCGCCAAGGAAGCCGGCGCCGATGTGATCCTCACCTACGCCATCGGCCCCGAG
CTGGCGCAGATCGCCAACGGCATGGCCAAGCTGGGCTGGAAGGTGCCCATGATCGGCTCCTGGACCCTGTCCATGGCCAG
CTTCATCGACACCGCCGGCCCCAACGGCGATGGCGCGCTGATGCCCCAGACCTTCATCCAGCTGCCCGATACCCCCAAGC
GCAAGGCCTTCATCGAGGCCTATCAGGCGGCCTACAAGATCGACCGCATGCCGTCCCCGGTGTCGGCCGCCCAGGGCTAT
GACTCGGTGTTCCTGCTCGCCGCAGCCATCACCCAGGCCGGCTCCACCGACGGCCGCAAGATCCGCGAAGCGCTGGAAAA
CCTGAACACCAAGGTGGAAGGCGTGGTCACCGTCTACGACCGCCCCTTCAGCGCCACTGACCATGAGGCCATCACCGGCA
ACATCCCGGTGTTCGGCCTGGTGAAGGGCGGCCGCGTGGTCGCCGCCCATCCCGAGGACATCGCCGGCGACAAGGCGGTC
CGGGTGAAGCCCAAGAACTGA

Upstream 100 bases:

>100_bases
GTCTATCGTCGCCAATTGGCTTAAACGATTCAGGCCTAACCGGCGAAGAAGCCGGATCGAACCAATAAAGCCTCAGAATC
GGGCAACCAGGGAGTGAATC

Downstream 100 bases:

>100_bases
GCGCGATCGGTCGCAACTGATCGGCCGGCAGCCTTTTCGCCGGCCGGCGACGAGAAGCCCGCCCGGTGGGCTTGCGCGTG
CCGGGTGGCGGCCTTTCCCG

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MRVSGMIAAVAASIAMAGVSIAGVAVAAEPIKIGLSGPFTGGSSSMGVSMRDGVKLAVTEINKNGGVLGRQLVLVERDDE
AKNEVGVQIAQELINKEGVVATLGYINTGVALASQRFYQEAEIPVINNVATGTLISKQFLPPDNKSNYIFRTSANDAIQS
VMIADEAVKRQGFKKPAILADSTNYGQLGRADLEKALAVLNVKPVVTEKFNIGDTDMTAQLLRAKEAGADVILTYAIGPE
LAQIANGMAKLGWKVPMIGSWTLSMASFIDTAGPNGDGALMPQTFIQLPDTPKRKAFIEAYQAAYKIDRMPSPVSAAQGY
DSVFLLAAAITQAGSTDGRKIREALENLNTKVEGVVTVYDRPFSATDHEAITGNIPVFGLVKGGRVVAAHPEDIAGDKAV
RVKPKN

Sequences:

>Translated_406_residues
MRVSGMIAAVAASIAMAGVSIAGVAVAAEPIKIGLSGPFTGGSSSMGVSMRDGVKLAVTEINKNGGVLGRQLVLVERDDE
AKNEVGVQIAQELINKEGVVATLGYINTGVALASQRFYQEAEIPVINNVATGTLISKQFLPPDNKSNYIFRTSANDAIQS
VMIADEAVKRQGFKKPAILADSTNYGQLGRADLEKALAVLNVKPVVTEKFNIGDTDMTAQLLRAKEAGADVILTYAIGPE
LAQIANGMAKLGWKVPMIGSWTLSMASFIDTAGPNGDGALMPQTFIQLPDTPKRKAFIEAYQAAYKIDRMPSPVSAAQGY
DSVFLLAAAITQAGSTDGRKIREALENLNTKVEGVVTVYDRPFSATDHEAITGNIPVFGLVKGGRVVAAHPEDIAGDKAV
RVKPKN
>Mature_406_residues
MRVSGMIAAVAASIAMAGVSIAGVAVAAEPIKIGLSGPFTGGSSSMGVSMRDGVKLAVTEINKNGGVLGRQLVLVERDDE
AKNEVGVQIAQELINKEGVVATLGYINTGVALASQRFYQEAEIPVINNVATGTLISKQFLPPDNKSNYIFRTSANDAIQS
VMIADEAVKRQGFKKPAILADSTNYGQLGRADLEKALAVLNVKPVVTEKFNIGDTDMTAQLLRAKEAGADVILTYAIGPE
LAQIANGMAKLGWKVPMIGSWTLSMASFIDTAGPNGDGALMPQTFIQLPDTPKRKAFIEAYQAAYKIDRMPSPVSAAQGY
DSVFLLAAAITQAGSTDGRKIREALENLNTKVEGVVTVYDRPFSATDHEAITGNIPVFGLVKGGRVVAAHPEDIAGDKAV
RVKPKN

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=337, Percent_Identity=24.6290801186944, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI48994941, Length=292, Percent_Identity=25.3424657534247, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 42788; Mature: 42788

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVSGMIAAVAASIAMAGVSIAGVAVAAEPIKIGLSGPFTGGSSSMGVSMRDGVKLAVTE
CCCCHHHHHHHHHHHHHCHHHHEEEEEECCEEEECCCCCCCCCCCCCEECCCCEEEEEEE
INKNGGVLGRQLVLVERDDEAKNEVGVQIAQELINKEGVVATLGYINTGVALASQRFYQE
ECCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHH
AEIPVINNVATGTLISKQFLPPDNKSNYIFRTSANDAIQSVMIADEAVKRQGFKKPAILA
CCCCEECCCCCCCCHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEE
DSTNYGQLGRADLEKALAVLNVKPVVTEKFNIGDTDMTAQLLRAKEAGADVILTYAIGPE
CCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCH
LAQIANGMAKLGWKVPMIGSWTLSMASFIDTAGPNGDGALMPQTFIQLPDTPKRKAFIEA
HHHHHHHHHHCCCCCCEECCHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCHHHHHHHH
YQAAYKIDRMPSPVSAAQGYDSVFLLAAAITQAGSTDGRKIREALENLNTKVEGVVTVYD
HHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEEEC
RPFSATDHEAITGNIPVFGLVKGGRVVAAHPEDIAGDKAVRVKPKN
CCCCCCCCCEEECCCCEEEEEECCEEEEECCCCCCCCCEEEEECCC
>Mature Secondary Structure
MRVSGMIAAVAASIAMAGVSIAGVAVAAEPIKIGLSGPFTGGSSSMGVSMRDGVKLAVTE
CCCCHHHHHHHHHHHHHCHHHHEEEEEECCEEEECCCCCCCCCCCCCEECCCCEEEEEEE
INKNGGVLGRQLVLVERDDEAKNEVGVQIAQELINKEGVVATLGYINTGVALASQRFYQE
ECCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHH
AEIPVINNVATGTLISKQFLPPDNKSNYIFRTSANDAIQSVMIADEAVKRQGFKKPAILA
CCCCEECCCCCCCCHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEE
DSTNYGQLGRADLEKALAVLNVKPVVTEKFNIGDTDMTAQLLRAKEAGADVILTYAIGPE
CCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCH
LAQIANGMAKLGWKVPMIGSWTLSMASFIDTAGPNGDGALMPQTFIQLPDTPKRKAFIEA
HHHHHHHHHHCCCCCCEECCHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCHHHHHHHH
YQAAYKIDRMPSPVSAAQGYDSVFLLAAAITQAGSTDGRKIREALENLNTKVEGVVTVYD
HHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEEEC
RPFSATDHEAITGNIPVFGLVKGGRVVAAHPEDIAGDKAVRVKPKN
CCCCCCCCCEEECCCCEEEEEECCEEEEECCCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]