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The map label for this gene is gatA

Identifier: 154247849

GI number: 154247849

Start: 4342569

End: 4344053

Strand: Direct

Name: gatA

Synonym: Xaut_3926

Alternate gene names: 154247849

Gene position: 4342569-4344053 (Clockwise)

Preceding gene: 154247848

Following gene: 154247851

Centisome position: 81.8

GC content: 68.69

Gene sequence:

>1485_bases
ATGACCAAGCTCACACAGCTCACCCTCACCCAGGCGCGCGAAGGCCTCGCCCAGGGCGAGTTTACCTCCGTCGAGCTTAC
CGAGGCCTATCTGAAGGCCATGGAGCAGGCCCGCCCGCTCAACGCCTATGTGCTGGAGACGCCCGAGATCGCCCTGGAAA
TGGCGAAGGAGAGCGATCGGCGCATCGCGTCCGGCGAGACCGGTCCGCTGGAGGGCATTCCCCTCGGCATCAAGGACATG
TTCGCCACCGAGGGCGTGCGCACCACGGCCTGCGCCAAGATCCTCGACAATTTCATCCCCCAGTACGAATCCACCGTCAC
CTCCCATCTGTGGCGGGACGGCGCGGTGCTGCTGGGCAAGCTCAACAATGACGAGTTCGCCATGGGCTCGTCCAACGAGA
CCTCGGCGTTCGGGCCCGTCGTCTCGCCGTGGCGGCGCGATGGCGACGAGACCCCGCTGGTGCCCGGTGGCTCGTCTGGC
GGCTCGGCGGCGGCGGTGGCGGCGTTCCTGTGCGCGGGCGCGACCGGCACCGACACCGGCGGCTCCATCCGCCAGCCGGC
GGCCTTCACCGGCACGGTCGGCATCAAGCCCACCTACGGGCGCTGCTCGCGCTGGGGCATTGTGGCCTTCGCCTCGTCCC
TCGACCAGGCCGGCCCGTTCGCCCGCACGGTGAACGACGCGGCGGTGCTGCTGAAGTCCATGGCGGGCTACGACCCCAAG
GATTCCACCTGCGTCAACCGGCCGGTGCCGGACTATGAGGAGGCGGTGGGCGCCTCCATCAAGGGCAAGAAGATCGGCAT
TCCGCGCGAATACCGCATGGACGGCATGTCCGACGAGATCGACGCCCTGTGGACCGAAGGCGCGCGCTTCCTGAAGGACG
CGGGAGCGGAGATCGTCGACATCTCGCTGCCCCACACCCAGTACGCGCTTCCGGCCTATTACATCGTGGCGCCGGCGGAG
GCCTCCTCCAACCTCGCCCGCTATGACGGGGTGCGCTTTGGCGAGCGCGTGCCGGGCCGCGACGTGGTGGAGATGTATGA
GAACACCCGCGCCGCCGGCTTCGGCCCGGAGGTGCGCCGCCGCATCATGATCGGCACCTATGTGCTCTCGGCCGGCTATT
ACGACGCCTATTACCTGCGCGCGCAGAAGGTGCGCAGCCTGATCAAGCGCGACTTCGACCAGGTCTTCGCCCAGGGCGTG
GACGCGGTGCTGACGCCGGCGACCCCGTCTCCCGCCTTCGGCATCGGTGAGAAGCTGAAGGCCGACCCGGTGGAGATGTA
TCTCAACGACGTCTTCACCGTGACCGTGAACATGGCCGGCCTGCCCGGCATCGCCGTCCCCGCCGGCCTTTCGGCGGACG
GGCTGCCCCTCGGCCTCCAGCTCATCGGCCGCCCGTTCGAGGAGGAGACCCTGTTCTCCCTCGCCGAGGTGATCGAGGAA
GCGGCGGGCCGATTCCCGGTGGACAAGCAGTGGTGGCGCGGCTGA

Upstream 100 bases:

>100_bases
TGCGCGAGGATGTGGTCACCGACGGCCACTGCCCCGAAAAGGTGCTTGCCAACGCGCCCGATGCCGAGGGTGGCTTCTTT
ACCGTGCCGAAGGTGGTGGA

Downstream 100 bases:

>100_bases
GTTGAGTCAAACGAGATCGGCGCCCGCACTTCGGTGCGGGTGCTTGGTGAACATTTGGACATTCACTCAGAGGGGCGTTT
GACGCCGTCGCAGGAGTATT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MTKLTQLTLTQAREGLAQGEFTSVELTEAYLKAMEQARPLNAYVLETPEIALEMAKESDRRIASGETGPLEGIPLGIKDM
FATEGVRTTACAKILDNFIPQYESTVTSHLWRDGAVLLGKLNNDEFAMGSSNETSAFGPVVSPWRRDGDETPLVPGGSSG
GSAAAVAAFLCAGATGTDTGGSIRQPAAFTGTVGIKPTYGRCSRWGIVAFASSLDQAGPFARTVNDAAVLLKSMAGYDPK
DSTCVNRPVPDYEEAVGASIKGKKIGIPREYRMDGMSDEIDALWTEGARFLKDAGAEIVDISLPHTQYALPAYYIVAPAE
ASSNLARYDGVRFGERVPGRDVVEMYENTRAAGFGPEVRRRIMIGTYVLSAGYYDAYYLRAQKVRSLIKRDFDQVFAQGV
DAVLTPATPSPAFGIGEKLKADPVEMYLNDVFTVTVNMAGLPGIAVPAGLSADGLPLGLQLIGRPFEEETLFSLAEVIEE
AAGRFPVDKQWWRG

Sequences:

>Translated_494_residues
MTKLTQLTLTQAREGLAQGEFTSVELTEAYLKAMEQARPLNAYVLETPEIALEMAKESDRRIASGETGPLEGIPLGIKDM
FATEGVRTTACAKILDNFIPQYESTVTSHLWRDGAVLLGKLNNDEFAMGSSNETSAFGPVVSPWRRDGDETPLVPGGSSG
GSAAAVAAFLCAGATGTDTGGSIRQPAAFTGTVGIKPTYGRCSRWGIVAFASSLDQAGPFARTVNDAAVLLKSMAGYDPK
DSTCVNRPVPDYEEAVGASIKGKKIGIPREYRMDGMSDEIDALWTEGARFLKDAGAEIVDISLPHTQYALPAYYIVAPAE
ASSNLARYDGVRFGERVPGRDVVEMYENTRAAGFGPEVRRRIMIGTYVLSAGYYDAYYLRAQKVRSLIKRDFDQVFAQGV
DAVLTPATPSPAFGIGEKLKADPVEMYLNDVFTVTVNMAGLPGIAVPAGLSADGLPLGLQLIGRPFEEETLFSLAEVIEE
AAGRFPVDKQWWRG
>Mature_493_residues
TKLTQLTLTQAREGLAQGEFTSVELTEAYLKAMEQARPLNAYVLETPEIALEMAKESDRRIASGETGPLEGIPLGIKDMF
ATEGVRTTACAKILDNFIPQYESTVTSHLWRDGAVLLGKLNNDEFAMGSSNETSAFGPVVSPWRRDGDETPLVPGGSSGG
SAAAVAAFLCAGATGTDTGGSIRQPAAFTGTVGIKPTYGRCSRWGIVAFASSLDQAGPFARTVNDAAVLLKSMAGYDPKD
STCVNRPVPDYEEAVGASIKGKKIGIPREYRMDGMSDEIDALWTEGARFLKDAGAEIVDISLPHTQYALPAYYIVAPAEA
SSNLARYDGVRFGERVPGRDVVEMYENTRAAGFGPEVRRRIMIGTYVLSAGYYDAYYLRAQKVRSLIKRDFDQVFAQGVD
AVLTPATPSPAFGIGEKLKADPVEMYLNDVFTVTVNMAGLPGIAVPAGLSADGLPLGLQLIGRPFEEETLFSLAEVIEEA
AGRFPVDKQWWRG

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=499, Percent_Identity=42.0841683366733, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI195972892, Length=509, Percent_Identity=24.75442043222, Blast_Score=112, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17543272, Length=455, Percent_Identity=36.7032967032967, Blast_Score=278, Evalue=5e-75,
Organism=Caenorhabditis elegans, GI17537465, Length=507, Percent_Identity=26.4299802761341, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17538252, Length=324, Percent_Identity=26.5432098765432, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=313, Percent_Identity=29.7124600638978, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17556264, Length=232, Percent_Identity=30.6034482758621, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=431, Percent_Identity=34.5707656612529, Blast_Score=237, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6319685, Length=471, Percent_Identity=27.3885350318471, Blast_Score=113, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24648113, Length=491, Percent_Identity=38.0855397148676, Blast_Score=309, Evalue=3e-84,
Organism=Drosophila melanogaster, GI161078093, Length=513, Percent_Identity=26.3157894736842, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24644968, Length=507, Percent_Identity=25.6410256410256, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24652985, Length=495, Percent_Identity=23.8383838383838, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19922090, Length=495, Percent_Identity=23.8383838383838, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24652981, Length=495, Percent_Identity=23.8383838383838, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24652983, Length=495, Percent_Identity=23.8383838383838, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI45550774, Length=251, Percent_Identity=31.4741035856574, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24648435, Length=251, Percent_Identity=31.4741035856574, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648437, Length=251, Percent_Identity=31.4741035856574, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21356731, Length=249, Percent_Identity=28.5140562248996, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_XANP2 (A7IMA7)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001418807.1
- ProteinModelPortal:   A7IMA7
- SMR:   A7IMA7
- STRING:   A7IMA7
- GeneID:   5422610
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_3926
- eggNOG:   COG0154
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- ProtClustDB:   PRK00012
- BioCyc:   XAUT78245:XAUT_3926-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 53089; Mature: 52957

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 78-78 ACT_SITE 158-158 ACT_SITE 182-182

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLTQLTLTQAREGLAQGEFTSVELTEAYLKAMEQARPLNAYVLETPEIALEMAKESDR
CCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHCC
RIASGETGPLEGIPLGIKDMFATEGVRTTACAKILDNFIPQYESTVTSHLWRDGAVLLGK
EECCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
LNNDEFAMGSSNETSAFGPVVSPWRRDGDETPLVPGGSSGGSAAAVAAFLCAGATGTDTG
CCCCCEECCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCC
GSIRQPAAFTGTVGIKPTYGRCSRWGIVAFASSLDQAGPFARTVNDAAVLLKSMAGYDPK
CCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
DSTCVNRPVPDYEEAVGASIKGKKIGIPREYRMDGMSDEIDALWTEGARFLKDAGAEIVD
CCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEE
ISLPHTQYALPAYYIVAPAEASSNLARYDGVRFGERVPGRDVVEMYENTRAAGFGPEVRR
EECCCCCCCCCEEEEEECCCCCCCHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHH
RIMIGTYVLSAGYYDAYYLRAQKVRSLIKRDFDQVFAQGVDAVLTPATPSPAFGIGEKLK
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCC
ADPVEMYLNDVFTVTVNMAGLPGIAVPAGLSADGLPLGLQLIGRPFEEETLFSLAEVIEE
CCHHHHHHHCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHH
AAGRFPVDKQWWRG
HHCCCCCCHHHCCC
>Mature Secondary Structure 
TKLTQLTLTQAREGLAQGEFTSVELTEAYLKAMEQARPLNAYVLETPEIALEMAKESDR
CCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHCC
RIASGETGPLEGIPLGIKDMFATEGVRTTACAKILDNFIPQYESTVTSHLWRDGAVLLGK
EECCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
LNNDEFAMGSSNETSAFGPVVSPWRRDGDETPLVPGGSSGGSAAAVAAFLCAGATGTDTG
CCCCCEECCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCC
GSIRQPAAFTGTVGIKPTYGRCSRWGIVAFASSLDQAGPFARTVNDAAVLLKSMAGYDPK
CCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
DSTCVNRPVPDYEEAVGASIKGKKIGIPREYRMDGMSDEIDALWTEGARFLKDAGAEIVD
CCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEE
ISLPHTQYALPAYYIVAPAEASSNLARYDGVRFGERVPGRDVVEMYENTRAAGFGPEVRR
EECCCCCCCCCEEEEEECCCCCCCHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHH
RIMIGTYVLSAGYYDAYYLRAQKVRSLIKRDFDQVFAQGVDAVLTPATPSPAFGIGEKLK
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCC
ADPVEMYLNDVFTVTVNMAGLPGIAVPAGLSADGLPLGLQLIGRPFEEETLFSLAEVIEE
CCHHHHHHHCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHH
AAGRFPVDKQWWRG
HHCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA