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The map label for this gene is lipA [H]

Identifier: 154247820

GI number: 154247820

Start: 4315444

End: 4316397

Strand: Direct

Name: lipA [H]

Synonym: Xaut_3897

Alternate gene names: 154247820

Gene position: 4315444-4316397 (Clockwise)

Preceding gene: 154247819

Following gene: 154247821

Centisome position: 81.29

GC content: 67.3

Gene sequence:

>954_bases
ATGGTCACGGTCATCGACACCCTCGCCCGCCCGCGCCACCCGGAAAAGGCCAACCGTCCGGAAACCGAGGTGCTGCGCAA
GCCCGACTGGATCCGCGTGAAGGCGCCGGGCTCGGCCGGCTGGTCGCAGACGGCGGAGATCGTGCGGGCCAACGGCCTGC
ACACCGTGTGCGAAGAGGCGGGCTGCCCCAATATCGGCGAGTGCTGGGAGAAGAAGCACGCCACCTTCATGATCATGGGC
GACACCTGCACGCGGGCCTGCGCCTTCTGCAACGTGCGCACCGGCATGCCGGAAGCGCTCGACCAGGGCGAACCGCAGAA
GGTGGGCGACGCCGTGGCCAAGCTCGGCCTCTCCCATGTGGTCATCACCTCCGTGGACCGGGACGACCTCGCCGACGGCG
GCGCCGAGCACTTCGCCCGTACCATCGCCGCCATCCGCAAGGCGAGCCCCGGCACCACCATCGAGATCCTCACCCCGGAC
TTCCTGCGCAAGGACGGGGCGCTTGAGGTGGTGGTGGCGGCCAGGCCCGACGTGTTCAACCACAATCTGGAAACGGTGCC
GGCCAAATATCTCTCGGTGCGGCCAGGCGCGCGCTATTTCCACTCCCTGCGCCTGCTGCAGAAGGTGAAGGAGCTGGACG
GCTCCATCTTCACCAAGTCCGGCATCATGGTGGGCCTCGGCGAGGAGCGGCCCGAAGTGCTCCAGCTGATGGACGACCTG
CGCTCGGCGGAGGTGGATTTCATCACCATCGGCCAGTATCTCCAGCCCACGCGCAAGCACCACAAGGTGGAGCGCTTCGT
GACGCCGGACGAGTTCAAGGCCTATGAGACGGTCGCCTATGCCAAGGGCTTCCTGATGGTTTCGGCGAGCCCGCTCACGC
GCTCGTCGCACCATGCGGGCGATGATTTCGAGAAATTGCGCGCGGCCCGGGTGGCGCGTCTCGGCCGGTCCTGA

Upstream 100 bases:

>100_bases
CCATGCCCGTCCGGCGCCTTGTCCGGGGCGGGCTTCGCCCGGCGCGGGCATTGCCCTGAAGGGCGTGCATCGTCCAGCCG
GGGGTTGAGAGACGAAACGC

Downstream 100 bases:

>100_bases
TCGTGCCATCCTTCTCCAACGCCCGGCAGGTGAGGCACGGGGCGCGGGACATGTTCGACCTCGTGGCCGACGTGGAGCGC
TATCCCGAGTTCGTGCCCCT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MVTVIDTLARPRHPEKANRPETEVLRKPDWIRVKAPGSAGWSQTAEIVRANGLHTVCEEAGCPNIGECWEKKHATFMIMG
DTCTRACAFCNVRTGMPEALDQGEPQKVGDAVAKLGLSHVVITSVDRDDLADGGAEHFARTIAAIRKASPGTTIEILTPD
FLRKDGALEVVVAARPDVFNHNLETVPAKYLSVRPGARYFHSLRLLQKVKELDGSIFTKSGIMVGLGEERPEVLQLMDDL
RSAEVDFITIGQYLQPTRKHHKVERFVTPDEFKAYETVAYAKGFLMVSASPLTRSSHHAGDDFEKLRAARVARLGRS

Sequences:

>Translated_317_residues
MVTVIDTLARPRHPEKANRPETEVLRKPDWIRVKAPGSAGWSQTAEIVRANGLHTVCEEAGCPNIGECWEKKHATFMIMG
DTCTRACAFCNVRTGMPEALDQGEPQKVGDAVAKLGLSHVVITSVDRDDLADGGAEHFARTIAAIRKASPGTTIEILTPD
FLRKDGALEVVVAARPDVFNHNLETVPAKYLSVRPGARYFHSLRLLQKVKELDGSIFTKSGIMVGLGEERPEVLQLMDDL
RSAEVDFITIGQYLQPTRKHHKVERFVTPDEFKAYETVAYAKGFLMVSASPLTRSSHHAGDDFEKLRAARVARLGRS
>Mature_317_residues
MVTVIDTLARPRHPEKANRPETEVLRKPDWIRVKAPGSAGWSQTAEIVRANGLHTVCEEAGCPNIGECWEKKHATFMIMG
DTCTRACAFCNVRTGMPEALDQGEPQKVGDAVAKLGLSHVVITSVDRDDLADGGAEHFARTIAAIRKASPGTTIEILTPD
FLRKDGALEVVVAARPDVFNHNLETVPAKYLSVRPGARYFHSLRLLQKVKELDGSIFTKSGIMVGLGEERPEVLQLMDDL
RSAEVDFITIGQYLQPTRKHHKVERFVTPDEFKAYETVAYAKGFLMVSASPLTRSSHHAGDDFEKLRAARVARLGRS

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=299, Percent_Identity=45.4849498327759, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI37577164, Length=253, Percent_Identity=46.2450592885375, Blast_Score=232, Evalue=3e-61,
Organism=Escherichia coli, GI1786846, Length=278, Percent_Identity=52.8776978417266, Blast_Score=297, Evalue=6e-82,
Organism=Caenorhabditis elegans, GI32564533, Length=258, Percent_Identity=44.9612403100775, Blast_Score=231, Evalue=5e-61,
Organism=Saccharomyces cerevisiae, GI6324770, Length=296, Percent_Identity=41.8918918918919, Blast_Score=246, Evalue=5e-66,
Organism=Drosophila melanogaster, GI221513272, Length=290, Percent_Identity=43.448275862069, Blast_Score=243, Evalue=1e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 34982; Mature: 34982

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTVIDTLARPRHPEKANRPETEVLRKPDWIRVKAPGSAGWSQTAEIVRANGLHTVCEEA
CEEEEHHHCCCCCCCCCCCCHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCHHHHHHHC
GCPNIGECWEKKHATFMIMGDTCTRACAFCNVRTGMPEALDQGEPQKVGDAVAKLGLSHV
CCCCHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHCCCEE
VITSVDRDDLADGGAEHFARTIAAIRKASPGTTIEILTPDFLRKDGALEVVVAARPDVFN
EEEECCHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEECHHHHCCCCCEEEEEECCCCHHC
HNLETVPAKYLSVRPGARYFHSLRLLQKVKELDGSIFTKSGIMVGLGEERPEVLQLMDDL
CCCHHCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEECCCEEEECCCCCHHHHHHHHHH
RSAEVDFITIGQYLQPTRKHHKVERFVTPDEFKAYETVAYAKGFLMVSASPLTRSSHHAG
HHCCCCEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCC
DDFEKLRAARVARLGRS
CHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVTVIDTLARPRHPEKANRPETEVLRKPDWIRVKAPGSAGWSQTAEIVRANGLHTVCEEA
CEEEEHHHCCCCCCCCCCCCHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCHHHHHHHC
GCPNIGECWEKKHATFMIMGDTCTRACAFCNVRTGMPEALDQGEPQKVGDAVAKLGLSHV
CCCCHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHCCCEE
VITSVDRDDLADGGAEHFARTIAAIRKASPGTTIEILTPDFLRKDGALEVVVAARPDVFN
EEEECCHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEECHHHHCCCCCEEEEEECCCCHHC
HNLETVPAKYLSVRPGARYFHSLRLLQKVKELDGSIFTKSGIMVGLGEERPEVLQLMDDL
CCCHHCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEECCCEEEECCCCCHHHHHHHHHH
RSAEVDFITIGQYLQPTRKHHKVERFVTPDEFKAYETVAYAKGFLMVSASPLTRSSHHAG
HHCCCCEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCC
DDFEKLRAARVARLGRS
CHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA