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The map label for this gene is pdhB [H]

Identifier: 154247813

GI number: 154247813

Start: 4309339

End: 4310709

Strand: Direct

Name: pdhB [H]

Synonym: Xaut_3890

Alternate gene names: 154247813

Gene position: 4309339-4310709 (Clockwise)

Preceding gene: 154247812

Following gene: 154247814

Centisome position: 81.17

GC content: 67.69

Gene sequence:

>1371_bases
ATGGCCATCGAAGTCCTCATGCCGGCCCTGTCTCCCACCATGGAGAAGGGCAATCTCACCAAGTGGCTCAAGAAGGAAGG
CGACACCGTCAAATCCGGTGACGTGCTTGCCGAGATCGAGACCGACAAGGCCACCATGGAGGTGGAGTCCATCGACGAAG
GAATCCTCGGCCGCATCCTCGTACCCGAGGGCGCCCAGGACGTGGCGGTGAACACCCCCATCGCCACCATCCTCGCCGAC
GGCGAAGATGCGAACGCCGCTCCGGCTCCCGCCCCCAAGGCGGCCGAGAGCGCCCCGGCCCCGGCCACAGCGCCCGCCCC
CGCGGCGCCGGCGGTGATCGCCCCGCAGGCGGTGGCCCAGCCCGATCCGGAAGTGCCGGCCGGCACCGAATTCGTCACCC
AGACCGTCCGCGAGGCCCTGCGCGACGCCATGGCCGAGGAAATGCGCCGCGACGGCGATGTCTTCGTCATGGGCGAGGAA
GTGGCCGAGTATCAGGGCGCCTACAAGATCACCCAGGGGCTGCTCCAGGAGTTCGGCGAGCGGCGCGTGATCGATACCCC
CATCACCGAGCATGGCTTTGCCGGCGTCGGCGTGGGTGCGGCCATGGCGGGCCTGAAGCCCATCATCGAGTTCATGACCT
TCAACTTCGCCATGCAGGCCATCGACCACATCATCAACTCGGCGGCGAAGACCCTGTATATGTCGGGCGGGCAGATGCAC
TGCTCCGTCGTGTTCCGCGGCCCCAACGGCGCCGCCGCCCGCGTCGCCGCCCAGCACAGCCAGGACTACACCTCCTGGTA
CTCCAACGTGCCCGGCCTGCGGGTGATCGCGCCCTATACGGCGGCCGACGCCAAGGGCCTCCTGAAGGCCGCCATCCGCG
ACCCCAACCCGGTCATCTTCCTTGAGAACGAGATCCTCTACGGCCACTCCTTCGAGGTGCCGAAGCTGGATGATTTCGTG
CTGCCCATCGGCAAGGCGCGCATCGCCCGGAGCGGCAAGGACGTGACCCTGGTTTCCTTCTCCATCGGCATGACCTATGC
GCTGAAGGCGGCGGACGAGCTGGCCAAGCAGGGCATCGAGGCGGAGGTCATCGACCTGCGCACCATCCGCCCCATGGACG
TGGACACCATCATCGCCTCGGTGAAGAAGACCGGCCGCTGCGTGAGCGTGGAAGAGGGCTGGCCGCAGTCCGGCGTCGGC
GCCGAGATCGTCGCCCAGCTCATGGACAAGGCCTTCGACTATCTCGACGCCCCGGTGCTGCGCGTCACGGGCAAGGACGT
GCCCATGCCGTACGCCGCCAACCTCGAGAAGCTGGCCCTGCCGACGGTCGCCGACGTGATCGCGGCCGTCCACGCCGTGA
CCTATCGCTGA

Upstream 100 bases:

>100_bases
CCATCTCCGCCTACACCAATCTCACCTACAAGCCCCCCGTGGCCCCGGCCGGCTCCGCGCCGGCCGCGCCCGCGGGCGCG
CCGAGCAAGAGCTGACAGCC

Downstream 100 bases:

>100_bases
GGAGAGAGATCCATGCCCATCGAGATCCTGATGCCGGCCCTCTCCCCCACCATGGAGAAGGGCAACCTCGCCAAGTGGCT
CAAGAAGGAAGGCGACACCG

Product: pyruvate dehydrogenase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MAIEVLMPALSPTMEKGNLTKWLKKEGDTVKSGDVLAEIETDKATMEVESIDEGILGRILVPEGAQDVAVNTPIATILAD
GEDANAAPAPAPKAAESAPAPATAPAPAAPAVIAPQAVAQPDPEVPAGTEFVTQTVREALRDAMAEEMRRDGDVFVMGEE
VAEYQGAYKITQGLLQEFGERRVIDTPITEHGFAGVGVGAAMAGLKPIIEFMTFNFAMQAIDHIINSAAKTLYMSGGQMH
CSVVFRGPNGAAARVAAQHSQDYTSWYSNVPGLRVIAPYTAADAKGLLKAAIRDPNPVIFLENEILYGHSFEVPKLDDFV
LPIGKARIARSGKDVTLVSFSIGMTYALKAADELAKQGIEAEVIDLRTIRPMDVDTIIASVKKTGRCVSVEEGWPQSGVG
AEIVAQLMDKAFDYLDAPVLRVTGKDVPMPYAANLEKLALPTVADVIAAVHAVTYR

Sequences:

>Translated_456_residues
MAIEVLMPALSPTMEKGNLTKWLKKEGDTVKSGDVLAEIETDKATMEVESIDEGILGRILVPEGAQDVAVNTPIATILAD
GEDANAAPAPAPKAAESAPAPATAPAPAAPAVIAPQAVAQPDPEVPAGTEFVTQTVREALRDAMAEEMRRDGDVFVMGEE
VAEYQGAYKITQGLLQEFGERRVIDTPITEHGFAGVGVGAAMAGLKPIIEFMTFNFAMQAIDHIINSAAKTLYMSGGQMH
CSVVFRGPNGAAARVAAQHSQDYTSWYSNVPGLRVIAPYTAADAKGLLKAAIRDPNPVIFLENEILYGHSFEVPKLDDFV
LPIGKARIARSGKDVTLVSFSIGMTYALKAADELAKQGIEAEVIDLRTIRPMDVDTIIASVKKTGRCVSVEEGWPQSGVG
AEIVAQLMDKAFDYLDAPVLRVTGKDVPMPYAANLEKLALPTVADVIAAVHAVTYR
>Mature_455_residues
AIEVLMPALSPTMEKGNLTKWLKKEGDTVKSGDVLAEIETDKATMEVESIDEGILGRILVPEGAQDVAVNTPIATILADG
EDANAAPAPAPKAAESAPAPATAPAPAAPAVIAPQAVAQPDPEVPAGTEFVTQTVREALRDAMAEEMRRDGDVFVMGEEV
AEYQGAYKITQGLLQEFGERRVIDTPITEHGFAGVGVGAAMAGLKPIIEFMTFNFAMQAIDHIINSAAKTLYMSGGQMHC
SVVFRGPNGAAARVAAQHSQDYTSWYSNVPGLRVIAPYTAADAKGLLKAAIRDPNPVIFLENEILYGHSFEVPKLDDFVL
PIGKARIARSGKDVTLVSFSIGMTYALKAADELAKQGIEAEVIDLRTIRPMDVDTIIASVKKTGRCVSVEEGWPQSGVGA
EIVAQLMDKAFDYLDAPVLRVTGKDVPMPYAANLEKLALPTVADVIAAVHAVTYR

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=320, Percent_Identity=59.6875, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI291084858, Length=320, Percent_Identity=56.5625, Blast_Score=374, Evalue=1e-103,
Organism=Homo sapiens, GI4557353, Length=315, Percent_Identity=35.8730158730159, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI34101272, Length=315, Percent_Identity=35.8730158730159, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI203098753, Length=88, Percent_Identity=50, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI203098816, Length=81, Percent_Identity=53.0864197530864, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI31711992, Length=84, Percent_Identity=51.1904761904762, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI260898739, Length=86, Percent_Identity=47.6744186046512, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI225637461, Length=241, Percent_Identity=32.7800829875519, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI225637459, Length=241, Percent_Identity=32.7800829875519, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI225637463, Length=241, Percent_Identity=32.7800829875519, Blast_Score=80, Evalue=6e-15,
Organism=Homo sapiens, GI133778974, Length=237, Percent_Identity=28.6919831223629, Blast_Score=73, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17538422, Length=322, Percent_Identity=63.6645962732919, Blast_Score=421, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17506935, Length=343, Percent_Identity=39.3586005830904, Blast_Score=205, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI17560088, Length=132, Percent_Identity=43.1818181818182, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319698, Length=326, Percent_Identity=61.0429447852761, Blast_Score=424, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6324258, Length=77, Percent_Identity=58.4415584415584, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321632, Length=111, Percent_Identity=44.1441441441441, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21358145, Length=319, Percent_Identity=62.0689655172414, Blast_Score=415, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24650940, Length=319, Percent_Identity=62.0689655172414, Blast_Score=415, Evalue=1e-116,
Organism=Drosophila melanogaster, GI160714832, Length=319, Percent_Identity=36.6771159874608, Blast_Score=201, Evalue=9e-52,
Organism=Drosophila melanogaster, GI160714828, Length=319, Percent_Identity=36.6771159874608, Blast_Score=200, Evalue=2e-51,
Organism=Drosophila melanogaster, GI24650943, Length=89, Percent_Identity=65.1685393258427, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24650945, Length=89, Percent_Identity=65.1685393258427, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI20129315, Length=78, Percent_Identity=52.5641025641026, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI45551847, Length=248, Percent_Identity=27.4193548387097, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI45550715, Length=248, Percent_Identity=27.4193548387097, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24645119, Length=248, Percent_Identity=27.4193548387097, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24582497, Length=67, Percent_Identity=49.2537313432836, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 48451; Mature: 48320

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIEVLMPALSPTMEKGNLTKWLKKEGDTVKSGDVLAEIETDKATMEVESIDEGILGRIL
CCHHEEHHHHCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCCEEEHHHHCCCCCCEEE
VPEGAQDVAVNTPIATILADGEDANAAPAPAPKAAESAPAPATAPAPAAPAVIAPQAVAQ
CCCCCCCEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCHHHCC
PDPEVPAGTEFVTQTVREALRDAMAEEMRRDGDVFVMGEEVAEYQGAYKITQGLLQEFGE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHCCHHHHHHHHHHHHCC
RRVIDTPITEHGFAGVGVGAAMAGLKPIIEFMTFNFAMQAIDHIINSAAKTLYMSGGQMH
CCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEE
CSVVFRGPNGAAARVAAQHSQDYTSWYSNVPGLRVIAPYTAADAKGLLKAAIRDPNPVIF
EEEEEECCCCCHHHHHHHHCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCCEEE
LENEILYGHSFEVPKLDDFVLPIGKARIARSGKDVTLVSFSIGMTYALKAADELAKQGIE
EECEEEECCCCCCCCCCCHHCCCCHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHCCCC
AEVIDLRTIRPMDVDTIIASVKKTGRCVSVEEGWPQSGVGAEIVAQLMDKAFDYLDAPVL
EEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEE
RVTGKDVPMPYAANLEKLALPTVADVIAAVHAVTYR
EECCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AIEVLMPALSPTMEKGNLTKWLKKEGDTVKSGDVLAEIETDKATMEVESIDEGILGRIL
CHHEEHHHHCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCCEEEHHHHCCCCCCEEE
VPEGAQDVAVNTPIATILADGEDANAAPAPAPKAAESAPAPATAPAPAAPAVIAPQAVAQ
CCCCCCCEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCHHHCC
PDPEVPAGTEFVTQTVREALRDAMAEEMRRDGDVFVMGEEVAEYQGAYKITQGLLQEFGE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHCCHHHHHHHHHHHHCC
RRVIDTPITEHGFAGVGVGAAMAGLKPIIEFMTFNFAMQAIDHIINSAAKTLYMSGGQMH
CCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEE
CSVVFRGPNGAAARVAAQHSQDYTSWYSNVPGLRVIAPYTAADAKGLLKAAIRDPNPVIF
EEEEEECCCCCHHHHHHHHCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCCEEE
LENEILYGHSFEVPKLDDFVLPIGKARIARSGKDVTLVSFSIGMTYALKAADELAKQGIE
EECEEEECCCCCCCCCCCHHCCCCHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHCCCC
AEVIDLRTIRPMDVDTIIASVKKTGRCVSVEEGWPQSGVGAEIVAQLMDKAFDYLDAPVL
EEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEE
RVTGKDVPMPYAANLEKLALPTVADVIAAVHAVTYR
EECCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]