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The map label for this gene is pdhC [H]

Identifier: 154247814

GI number: 154247814

Start: 4310722

End: 4312068

Strand: Direct

Name: pdhC [H]

Synonym: Xaut_3891

Alternate gene names: 154247814

Gene position: 4310722-4312068 (Clockwise)

Preceding gene: 154247813

Following gene: 154247815

Centisome position: 81.2

GC content: 68.6

Gene sequence:

>1347_bases
ATGCCCATCGAGATCCTGATGCCGGCCCTCTCCCCCACCATGGAGAAGGGCAACCTCGCCAAGTGGCTCAAGAAGGAAGG
CGACACCGTCAAGTCCGGTGACGTTCTTGCCGAGATCGAGACCGACAAGGCGACCATGGAAGTCGAGTCCATCGACGAAG
GCATCCTCGCCAAGATCCTGGTGCCGGAAGGCTCTCAGGACGTGCCGGTGAACCAGCTCATCGCCTTGCTGGCCGGTGAG
GGCGAGGACGTGGCCGCCGCTGCCGCCGGTGGCGGTGCCAAGGCCGCTGCCGCTCCTGCGGCCGCTGCCGCGCCTGCGGC
TGCTGCGCCCGCCGCTGCTGCGCCCGCCGCGGCTCCGGCGCCTGCCGCCGCTCCTGCCTCCAACGGCCAGGGCGGACGCG
TGTTCGCCTCGCCGCTCGCCCGCCGGATCGCCAAGGACAAGGGCATCGACCTTGCCGCCCTCGCCGGCTCCGGCCCGCAC
GGCCGCATCGTCGCCCGCGACCTGGAAGGCGCCAAGCCCGGCGCCAAGCCGGCCGCTGCGCCCGCCGCTGCCGCCACTCC
CGCGCCTGCCGCTGCTCCCGCCCCCAAGGCCGCGGTGGCCAGCGCGCCCGGCGCCGAGCAGGTCAAGGCCATGTTCGAGG
CCGGCAGCTACGAGGAAGTGCAGCTCGACGGCATGCGCAAGACCATTGCGCGCCGCCTGGTGGAGAGCGAGCAGGTGACC
CCCACCTTCTTCCTCACCGTGGACTGCGACCTCGACGACCTCATGGCCCTGCGCGAGCAGGTGAACGCCAATGCGTCCAA
GGACAAGGACGGCAAGCCGTCCTACCGTGTCTCGGTCAACGACTTCATCATCAAGGCCATGGCGCTGGCGCTCCAGAAGG
TGCCCGCCGCCAATGCGGTGTGGGCCGAAGACCGCATCCTGCGCATGAAGCATTCGGACGTGGGCGTGGCGGTGGCCATT
GACGGCGGTCTCTACGCTCCCATCGTGAAGAAGGCGGAGCAGAAGACCCTGTCCGCCATCTCCAACGAGATGCGCGACCT
TGCCGGCCGTGCCCGCACCAAGAAGCTGAAGCCGGATGAATATTCCGGCGGCTCCACCTCGGTGTCGAACCTCGGCATGA
TGGGAATCCGGAACTTCACCGCCATCATCAACGCGCCGCAGTCCTCCATCCTCGCGGTGGGCGCTTCGGAGCAGCGCGCC
GTGGTCCGCAATGGCGAGATCAAGGCGGTGATGCAGATGACCGTCACCATGACCTGTGACCACCGGGTGATGGATGGCGC
GCTCGGCGCCGAGCTGCTCAGCGCGTTCAAGGGCTTCATCGAGAAGCCCATGAGCATGCTGGTGTGA

Upstream 100 bases:

>100_bases
CATGCCGTACGCCGCCAACCTCGAGAAGCTGGCCCTGCCGACGGTCGCCGACGTGATCGCGGCCGTCCACGCCGTGACCT
ATCGCTGAGGAGAGAGATCC

Downstream 100 bases:

>100_bases
GCACGCCGGTGTGAGGCTCGCCCCGGTGCTGCGGCAGATCAAATCCTGCCGCAGCCGCACGGTGTAGCTGCGTGGCAACG
GTGTTTTCCGCCGTTGTACC

Product: pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MPIEILMPALSPTMEKGNLAKWLKKEGDTVKSGDVLAEIETDKATMEVESIDEGILAKILVPEGSQDVPVNQLIALLAGE
GEDVAAAAAGGGAKAAAAPAAAAAPAAAAPAAAAPAAAPAPAAAPASNGQGGRVFASPLARRIAKDKGIDLAALAGSGPH
GRIVARDLEGAKPGAKPAAAPAAAATPAPAAAPAPKAAVASAPGAEQVKAMFEAGSYEEVQLDGMRKTIARRLVESEQVT
PTFFLTVDCDLDDLMALREQVNANASKDKDGKPSYRVSVNDFIIKAMALALQKVPAANAVWAEDRILRMKHSDVGVAVAI
DGGLYAPIVKKAEQKTLSAISNEMRDLAGRARTKKLKPDEYSGGSTSVSNLGMMGIRNFTAIINAPQSSILAVGASEQRA
VVRNGEIKAVMQMTVTMTCDHRVMDGALGAELLSAFKGFIEKPMSMLV

Sequences:

>Translated_448_residues
MPIEILMPALSPTMEKGNLAKWLKKEGDTVKSGDVLAEIETDKATMEVESIDEGILAKILVPEGSQDVPVNQLIALLAGE
GEDVAAAAAGGGAKAAAAPAAAAAPAAAAPAAAAPAAAPAPAAAPASNGQGGRVFASPLARRIAKDKGIDLAALAGSGPH
GRIVARDLEGAKPGAKPAAAPAAAATPAPAAAPAPKAAVASAPGAEQVKAMFEAGSYEEVQLDGMRKTIARRLVESEQVT
PTFFLTVDCDLDDLMALREQVNANASKDKDGKPSYRVSVNDFIIKAMALALQKVPAANAVWAEDRILRMKHSDVGVAVAI
DGGLYAPIVKKAEQKTLSAISNEMRDLAGRARTKKLKPDEYSGGSTSVSNLGMMGIRNFTAIINAPQSSILAVGASEQRA
VVRNGEIKAVMQMTVTMTCDHRVMDGALGAELLSAFKGFIEKPMSMLV
>Mature_447_residues
PIEILMPALSPTMEKGNLAKWLKKEGDTVKSGDVLAEIETDKATMEVESIDEGILAKILVPEGSQDVPVNQLIALLAGEG
EDVAAAAAGGGAKAAAAPAAAAAPAAAAPAAAAPAAAPAPAAAPASNGQGGRVFASPLARRIAKDKGIDLAALAGSGPHG
RIVARDLEGAKPGAKPAAAPAAAATPAPAAAPAPKAAVASAPGAEQVKAMFEAGSYEEVQLDGMRKTIARRLVESEQVTP
TFFLTVDCDLDDLMALREQVNANASKDKDGKPSYRVSVNDFIIKAMALALQKVPAANAVWAEDRILRMKHSDVGVAVAID
GGLYAPIVKKAEQKTLSAISNEMRDLAGRARTKKLKPDEYSGGSTSVSNLGMMGIRNFTAIINAPQSSILAVGASEQRAV
VRNGEIKAVMQMTVTMTCDHRVMDGALGAELLSAFKGFIEKPMSMLV

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=458, Percent_Identity=41.2663755458515, Blast_Score=330, Evalue=1e-90,
Organism=Homo sapiens, GI203098753, Length=476, Percent_Identity=35.9243697478992, Blast_Score=266, Evalue=3e-71,
Organism=Homo sapiens, GI203098816, Length=476, Percent_Identity=35.9243697478992, Blast_Score=266, Evalue=4e-71,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=40.3726708074534, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI110671329, Length=444, Percent_Identity=24.0990990990991, Blast_Score=113, Evalue=5e-25,
Organism=Homo sapiens, GI19923748, Length=230, Percent_Identity=28.695652173913, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI1786946, Length=446, Percent_Identity=29.1479820627803, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1786305, Length=414, Percent_Identity=33.0917874396135, Blast_Score=151, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI17560088, Length=457, Percent_Identity=43.7636761487965, Blast_Score=344, Evalue=4e-95,
Organism=Caenorhabditis elegans, GI17538894, Length=312, Percent_Identity=32.6923076923077, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI25146366, Length=448, Percent_Identity=28.7946428571429, Blast_Score=148, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI17537937, Length=437, Percent_Identity=26.3157894736842, Blast_Score=137, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6324258, Length=462, Percent_Identity=42.8571428571429, Blast_Score=308, Evalue=1e-84,
Organism=Saccharomyces cerevisiae, GI6320352, Length=453, Percent_Identity=28.0353200883002, Blast_Score=147, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6321632, Length=172, Percent_Identity=33.7209302325581, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI20129315, Length=462, Percent_Identity=42.2077922077922, Blast_Score=285, Evalue=4e-77,
Organism=Drosophila melanogaster, GI24582497, Length=451, Percent_Identity=41.6851441241685, Blast_Score=270, Evalue=2e-72,
Organism=Drosophila melanogaster, GI18859875, Length=439, Percent_Identity=28.4738041002278, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24645909, Length=246, Percent_Identity=30.0813008130081, Blast_Score=102, Evalue=5e-22,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 45952; Mature: 45821

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIEILMPALSPTMEKGNLAKWLKKEGDTVKSGDVLAEIETDKATMEVESIDEGILAKIL
CCCEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCCEEEHHHCCCCCEEEEE
VPEGSQDVPVNQLIALLAGEGEDVAAAAAGGGAKAAAAPAAAAAPAAAAPAAAAPAAAPA
CCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCCHHCCCCHHCCCCCCCCCCCCCCCCCC
PAAAPASNGQGGRVFASPLARRIAKDKGIDLAALAGSGPHGRIVARDLEGAKPGAKPAAA
CCCCCCCCCCCCEEHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEECCCCCCCCCCCCCC
PAAAATPAPAAAPAPKAAVASAPGAEQVKAMFEAGSYEEVQLDGMRKTIARRLVESEQVT
CCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCC
PTFFLTVDCDLDDLMALREQVNANASKDKDGKPSYRVSVNDFIIKAMALALQKVPAANAV
EEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCE
WAEDRILRMKHSDVGVAVAIDGGLYAPIVKKAEQKTLSAISNEMRDLAGRARTKKLKPDE
ECCCCEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
YSGGSTSVSNLGMMGIRNFTAIINAPQSSILAVGASEQRAVVRNGEIKAVMQMTVTMTCD
CCCCCCCHHHCCHHHHHHHHHEEECCCCCEEEECCCCCCHHHCCCCEEEEEEEEEEEECC
HRVMDGALGAELLSAFKGFIEKPMSMLV
CHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PIEILMPALSPTMEKGNLAKWLKKEGDTVKSGDVLAEIETDKATMEVESIDEGILAKIL
CCEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCCEEEHHHCCCCCEEEEE
VPEGSQDVPVNQLIALLAGEGEDVAAAAAGGGAKAAAAPAAAAAPAAAAPAAAAPAAAPA
CCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCCHHCCCCHHCCCCCCCCCCCCCCCCCC
PAAAPASNGQGGRVFASPLARRIAKDKGIDLAALAGSGPHGRIVARDLEGAKPGAKPAAA
CCCCCCCCCCCCEEHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEECCCCCCCCCCCCCC
PAAAATPAPAAAPAPKAAVASAPGAEQVKAMFEAGSYEEVQLDGMRKTIARRLVESEQVT
CCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCC
PTFFLTVDCDLDDLMALREQVNANASKDKDGKPSYRVSVNDFIIKAMALALQKVPAANAV
EEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCE
WAEDRILRMKHSDVGVAVAIDGGLYAPIVKKAEQKTLSAISNEMRDLAGRARTKKLKPDE
ECCCCEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
YSGGSTSVSNLGMMGIRNFTAIINAPQSSILAVGASEQRAVVRNGEIKAVMQMTVTMTCD
CCCCCCCHHHCCHHHHHHHHHEEECCCCCEEEECCCCCCHHHCCCCEEEEEEEEEEEECC
HRVMDGALGAELLSAFKGFIEKPMSMLV
CHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]