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The map label for this gene is pcaB [H]

Identifier: 154246625

GI number: 154246625

Start: 2974619

End: 2975680

Strand: Reverse

Name: pcaB [H]

Synonym: Xaut_2685

Alternate gene names: 154246625

Gene position: 2975680-2974619 (Counterclockwise)

Preceding gene: 154246626

Following gene: 154246623

Centisome position: 56.05

GC content: 72.6

Gene sequence:

>1062_bases
ATGAGCGTGTCCGTGTTCGAGCACCCCTTCCTGCGGCCACTCCTTGGCGATGATGCCATCGCCGCGGCCTTCACCGCCGA
AGCCGACATCGCCGCCATGCTCCGTTTCGAAGCCGCGCTGGCGGAGGCGGAGGCGGAGGCCGGCGTCATTCCGCACGAGG
CGGCCGCTGCCATCGCGCAGGCGGCACGCAGCATCGCCGTCGCACCCGCGGATCTGGCCGCCGCAGTGGCCCGCGACGGC
GTGGTGATCCCGGAGCTGGTGCGCCGGCTGCGCGCGGCGGTGGGCGAGCCCCATGCGGGCTTCGTGCACAAGGGCTCGAC
CAGCCAGGATGTGATCGACACCAGCCTGTTCCTGCGCCTGAAGGCGGCCTCCGACATCCTGCTGGCACGCCTCGACGCGC
TGGTCGGCAGCTTCGATCGTCTCGCCTCCACCACCGGCGCTCTGGCGCTGACGGGCCATACGCGCATGCAGCGGGCGCGG
CCCATCACCCTCGCCCACAAGGTTGCCACCTGGCGCGAGCCCCTGGCGCGGCACCGGGCCCGTTTTCCGGCGGTGGCGGA
CGATCTGTTCCGGGTGCAGCTCGGGGGCGCTGTGGGCAACCGGGCCGAGCTGGGCGAAGCCGCCGGGCGCGTGGCAGACG
GCCTCGCAACCCGGCTCGGCCTAGGCGCCGCGCCGCGCGCCACCCATGCCGAGCGGGACGCTATCGCCGCCTATGGGCAT
TGGTGCGCGCTGCTGGCCGGTTCGCTTGGCAAGTTCGGCGCCGATATCGCCCTCATGGCCCAGAACGAGGTGGGCGAGGT
GACCATCGCCGGCGGCGGCGGCTCCTCCGCGATGCCGGAGAAGAACAATCCCGTGGGCGCCGAGATCCTGGTGACCCTGG
CGCGCTTCGCCGCCACCCTCCAGGGCGGCCTCGACCAGGCCCTGGTGCACGAGAACGAGCGGTCCGGCGCCGCCTGGACC
CTGGAGTGGATGATCCTGCCCCAGTTGGTGACGACCGCCGGCGCGGCCACCCGCACCGCCTTCGCGCTCATCGAGGCCCT
GCGTTTTCCTGCAGCATCCTGA

Upstream 100 bases:

>100_bases
GCCCGCGCCCTGTGAGCCGGACCACACAACAGGCTTGACGCCAGAGCGCGCCGCAGTGGAGCTTGCGGCCCGTTTCGTTC
CCGAGGAAGGCCCCAGATCC

Downstream 100 bases:

>100_bases
CGACGCGGCGGCGGCCGACGGGCGGCAACGGGCTTCAGAATGGCAGGCCCACGTAGTTCTCGGCCACCGTGGTCGCCGCC
GCCTCCGAGCCGGCGATATA

Product: 3-carboxy-cis,cis-muconate cycloisomerase

Products: NA

Alternate protein names: 3-carboxymuconate lactonizing enzyme; CMLE [H]

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MSVSVFEHPFLRPLLGDDAIAAAFTAEADIAAMLRFEAALAEAEAEAGVIPHEAAAAIAQAARSIAVAPADLAAAVARDG
VVIPELVRRLRAAVGEPHAGFVHKGSTSQDVIDTSLFLRLKAASDILLARLDALVGSFDRLASTTGALALTGHTRMQRAR
PITLAHKVATWREPLARHRARFPAVADDLFRVQLGGAVGNRAELGEAAGRVADGLATRLGLGAAPRATHAERDAIAAYGH
WCALLAGSLGKFGADIALMAQNEVGEVTIAGGGGSSAMPEKNNPVGAEILVTLARFAATLQGGLDQALVHENERSGAAWT
LEWMILPQLVTTAGAATRTAFALIEALRFPAAS

Sequences:

>Translated_353_residues
MSVSVFEHPFLRPLLGDDAIAAAFTAEADIAAMLRFEAALAEAEAEAGVIPHEAAAAIAQAARSIAVAPADLAAAVARDG
VVIPELVRRLRAAVGEPHAGFVHKGSTSQDVIDTSLFLRLKAASDILLARLDALVGSFDRLASTTGALALTGHTRMQRAR
PITLAHKVATWREPLARHRARFPAVADDLFRVQLGGAVGNRAELGEAAGRVADGLATRLGLGAAPRATHAERDAIAAYGH
WCALLAGSLGKFGADIALMAQNEVGEVTIAGGGGSSAMPEKNNPVGAEILVTLARFAATLQGGLDQALVHENERSGAAWT
LEWMILPQLVTTAGAATRTAFALIEALRFPAAS
>Mature_352_residues
SVSVFEHPFLRPLLGDDAIAAAFTAEADIAAMLRFEAALAEAEAEAGVIPHEAAAAIAQAARSIAVAPADLAAAVARDGV
VIPELVRRLRAAVGEPHAGFVHKGSTSQDVIDTSLFLRLKAASDILLARLDALVGSFDRLASTTGALALTGHTRMQRARP
ITLAHKVATWREPLARHRARFPAVADDLFRVQLGGAVGNRAELGEAAGRVADGLATRLGLGAAPRATHAERDAIAAYGHW
CALLAGSLGKFGADIALMAQNEVGEVTIAGGGGSSAMPEKNNPVGAEILVTLARFAATLQGGLDQALVHENERSGAAWTL
EWMILPQLVTTAGAATRTAFALIEALRFPAAS

Specific function: Catalyzes an anti cycloisomerization [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family [H]

Homologues:

Organism=Homo sapiens, GI68303547, Length=209, Percent_Identity=28.2296650717703, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI31541964, Length=209, Percent_Identity=28.2296650717703, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI68303542, Length=209, Percent_Identity=28.2296650717703, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1790398, Length=197, Percent_Identity=28.9340101522843, Blast_Score=62, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6321806, Length=207, Percent_Identity=27.0531400966184, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR012789 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =5.5.1.2 [H]

Molecular weight: Translated: 36566; Mature: 36435

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSVFEHPFLRPLLGDDAIAAAFTAEADIAAMLRFEAALAEAEAEAGVIPHEAAAAIAQ
CCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
AARSIAVAPADLAAAVARDGVVIPELVRRLRAAVGEPHAGFVHKGSTSQDVIDTSLFLRL
HHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHEE
KAASDILLARLDALVGSFDRLASTTGALALTGHTRMQRARPITLAHKVATWREPLARHRA
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHH
RFPAVADDLFRVQLGGAVGNRAELGEAAGRVADGLATRLGLGAAPRATHAERDAIAAYGH
CCCHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
WCALLAGSLGKFGADIALMAQNEVGEVTIAGGGGSSAMPEKNNPVGAEILVTLARFAATL
HHHHHHHHHHHCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
QGGLDQALVHENERSGAAWTLEWMILPQLVTTAGAATRTAFALIEALRFPAAS
HCCHHHHHHCCCCCCCCEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SVSVFEHPFLRPLLGDDAIAAAFTAEADIAAMLRFEAALAEAEAEAGVIPHEAAAAIAQ
CCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
AARSIAVAPADLAAAVARDGVVIPELVRRLRAAVGEPHAGFVHKGSTSQDVIDTSLFLRL
HHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHEE
KAASDILLARLDALVGSFDRLASTTGALALTGHTRMQRARPITLAHKVATWREPLARHRA
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHH
RFPAVADDLFRVQLGGAVGNRAELGEAAGRVADGLATRLGLGAAPRATHAERDAIAAYGH
CCCHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
WCALLAGSLGKFGADIALMAQNEVGEVTIAGGGGSSAMPEKNNPVGAEILVTLARFAATL
HHHHHHHHHHHCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
QGGLDQALVHENERSGAAWTLEWMILPQLVTTAGAATRTAFALIEALRFPAAS
HCCHHHHHHCCCCCCCCEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1390752 [H]