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The map label for this gene is yjeA [H]

Identifier: 154246471

GI number: 154246471

Start: 2827403

End: 2828494

Strand: Reverse

Name: yjeA [H]

Synonym: Xaut_2530

Alternate gene names: 154246471

Gene position: 2828494-2827403 (Counterclockwise)

Preceding gene: 154246474

Following gene: 154246463

Centisome position: 53.28

GC content: 71.34

Gene sequence:

>1092_bases
ATGGCGGTATCGACGCCCGGCACAAGCCCCGATCCCAGCGCCAATCCCAGCCCCAATCCCAGCCCATGGTGGAGCCGCTC
CGTCCACGCCGACCGGCGGCCGTTCCTGCTGGCGCGCGGGCGCATGATGGCGGCGATCCGCGCCCGCCTTGCCGGCGAAG
GCTTCATTGAGGTGGAGACGCCGGCGCTCCAGGTCTCGCCCGGCAACGAGGCGCACCTGCACGCCTTCGCCACCGACCTC
ATCGGCCCGGACGGTGCCCGGCGCACGCTCTATCTGCGCACGTCGCCGGAATTCGCCTCCAAGAAGCTGCTGGCGGCGGG
GGAAGAGCAGATCTTCGAGTTCGCCCGTGTGTTCCGCAACCGGGAGCGCGGCATGGCCCACCATCCCGAGTTCACCCTGC
TGGAATGGTATCGGGCCGGTGCGCCCTATAACCGGCTCATGGACGACTGTGCCGGCCTTCTCGCCTGTGCCGCCGAGGCC
GGCGGCGTGTCGACCTTCGCCTGGCGGGGACACACCTGCGACCCCTTCGCCGCGCCGGAGCGGCTGACGCTGGAAGAGGC
GTTCGCGCGCCACGCCGGCATGGCTCTGGCCACGGTGCTGCCCGCCCCCGGCGAGCGGCCCGATCCGAGGCCGTTTGCCG
AGCTGGCGGCGGCGCACGGCATCCGCACCGCGCCGGACGACACCTGGGCGGACATCTTCTCGCGGGTGCTGGTGGAAAAG
ATCGAGACCCGGCTCGGCATGGGGCGGCCCACGGTGCTGTGCGACTATCCGGTGAGCGAGGCGGCGCTGGCCCGGCCCAA
GCCGGAGGATCCGCGCTTCGCCGAGCGCTTCGAGCTTTATGCCTGCGGGCTGGAGCTGGCCAATGCGTTCGGCGAACTGA
CCGACGCCGACGAGCAGCGCCGGCGCTTCGAGGAGGAGATGGACGAGAAAGAACGGCTCTATGGCGAGCGCTATCCCATC
GATGCCGATTTCCTGGCCGCTCTGGCGGCGATGCCGCCCGCGAGCGGCTGCGCCCTGGGCTTCGACCGCCTCGCCGTTCT
GGCCAGCGGCGCGCGTCGCATCGAGGACGTGCTGTGGGCACCGGTGGGCTGA

Upstream 100 bases:

>100_bases
GATCTATCGGCGCGCAACATAGCGATCTTGGCGCACAAAACCAGTCTCGTGCCCGCCTTTCTGCCTCCGGCCCCCTGCCG
CAATAAGGATTTCGCGCCCC

Downstream 100 bases:

>100_bases
TGCCAGCTCAGGCGCCGAGCGGGCTAGCTCAGGCCACGCTCAGGCGCCGCGCGGGCTCAGGTGATCTCGAACAGGGTCGA
GAAGCCGGAAATGTCGAACA

Product: lysine--tRNA ligase

Products: AMP; diphosphate; L-lysyl-tRNALys [C]

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MAVSTPGTSPDPSANPSPNPSPWWSRSVHADRRPFLLARGRMMAAIRARLAGEGFIEVETPALQVSPGNEAHLHAFATDL
IGPDGARRTLYLRTSPEFASKKLLAAGEEQIFEFARVFRNRERGMAHHPEFTLLEWYRAGAPYNRLMDDCAGLLACAAEA
GGVSTFAWRGHTCDPFAAPERLTLEEAFARHAGMALATVLPAPGERPDPRPFAELAAAHGIRTAPDDTWADIFSRVLVEK
IETRLGMGRPTVLCDYPVSEAALARPKPEDPRFAERFELYACGLELANAFGELTDADEQRRRFEEEMDEKERLYGERYPI
DADFLAALAAMPPASGCALGFDRLAVLASGARRIEDVLWAPVG

Sequences:

>Translated_363_residues
MAVSTPGTSPDPSANPSPNPSPWWSRSVHADRRPFLLARGRMMAAIRARLAGEGFIEVETPALQVSPGNEAHLHAFATDL
IGPDGARRTLYLRTSPEFASKKLLAAGEEQIFEFARVFRNRERGMAHHPEFTLLEWYRAGAPYNRLMDDCAGLLACAAEA
GGVSTFAWRGHTCDPFAAPERLTLEEAFARHAGMALATVLPAPGERPDPRPFAELAAAHGIRTAPDDTWADIFSRVLVEK
IETRLGMGRPTVLCDYPVSEAALARPKPEDPRFAERFELYACGLELANAFGELTDADEQRRRFEEEMDEKERLYGERYPI
DADFLAALAAMPPASGCALGFDRLAVLASGARRIEDVLWAPVG
>Mature_362_residues
AVSTPGTSPDPSANPSPNPSPWWSRSVHADRRPFLLARGRMMAAIRARLAGEGFIEVETPALQVSPGNEAHLHAFATDLI
GPDGARRTLYLRTSPEFASKKLLAAGEEQIFEFARVFRNRERGMAHHPEFTLLEWYRAGAPYNRLMDDCAGLLACAAEAG
GVSTFAWRGHTCDPFAAPERLTLEEAFARHAGMALATVLPAPGERPDPRPFAELAAAHGIRTAPDDTWADIFSRVLVEKI
ETRLGMGRPTVLCDYPVSEAALARPKPEDPRFAERFELYACGLELANAFGELTDADEQRRRFEEEMDEKERLYGERYPID
ADFLAALAAMPPASGCALGFDRLAVLASGARRIEDVLWAPVG

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=340, Percent_Identity=29.1176470588235, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI5031815, Length=340, Percent_Identity=29.1176470588235, Blast_Score=136, Evalue=2e-32,
Organism=Escherichia coli, GI87082379, Length=329, Percent_Identity=36.4741641337386, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1790571, Length=339, Percent_Identity=32.7433628318584, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1789256, Length=344, Percent_Identity=31.1046511627907, Blast_Score=154, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI71994340, Length=339, Percent_Identity=29.4985250737463, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17535927, Length=339, Percent_Identity=29.4985250737463, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17535925, Length=339, Percent_Identity=29.4985250737463, Blast_Score=130, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6320242, Length=353, Percent_Identity=27.7620396600567, Blast_Score=123, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24640851, Length=348, Percent_Identity=28.1609195402299, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24640849, Length=348, Percent_Identity=28.1609195402299, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6 [C]

Molecular weight: Translated: 39844; Mature: 39713

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVSTPGTSPDPSANPSPNPSPWWSRSVHADRRPFLLARGRMMAAIRARLAGEGFIEVET
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCEEEEEC
PALQVSPGNEAHLHAFATDLIGPDGARRTLYLRTSPEFASKKLLAAGEEQIFEFARVFRN
CEEEECCCCCHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHH
RERGMAHHPEFTLLEWYRAGAPYNRLMDDCAGLLACAAEAGGVSTFAWRGHTCDPFAAPE
HHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCC
RLTLEEAFARHAGMALATVLPAPGERPDPRPFAELAAAHGIRTAPDDTWADIFSRVLVEK
HHHHHHHHHHHHCHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
IETRLGMGRPTVLCDYPVSEAALARPKPEDPRFAERFELYACGLELANAFGELTDADEQR
HHHHHCCCCCCEEECCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
RRFEEEMDEKERLYGERYPIDADFLAALAAMPPASGCALGFDRLAVLASGARRIEDVLWA
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
PVG
CCC
>Mature Secondary Structure 
AVSTPGTSPDPSANPSPNPSPWWSRSVHADRRPFLLARGRMMAAIRARLAGEGFIEVET
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCEEEEEC
PALQVSPGNEAHLHAFATDLIGPDGARRTLYLRTSPEFASKKLLAAGEEQIFEFARVFRN
CEEEECCCCCHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHH
RERGMAHHPEFTLLEWYRAGAPYNRLMDDCAGLLACAAEAGGVSTFAWRGHTCDPFAAPE
HHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCC
RLTLEEAFARHAGMALATVLPAPGERPDPRPFAELAAAHGIRTAPDDTWADIFSRVLVEK
HHHHHHHHHHHHCHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
IETRLGMGRPTVLCDYPVSEAALARPKPEDPRFAERFELYACGLELANAFGELTDADEQR
HHHHHCCCCCCEEECCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
RRFEEEMDEKERLYGERYPIDADFLAALAAMPPASGCALGFDRLAVLASGARRIEDVLWA
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
PVG
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNALys [C]

Specific reaction: ATP + L-lysine + tRNALys = AMP + diphosphate + L-lysyl-tRNALys [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA